Presence of vascular endothelial growth factor during the first half of IVM improves the meiotic and developmental competence of porcine oocytes from small follicles
Tra M. T. Bui A , Khánh X. Nguyễn A , Asako Karata A , Pilar Ferré A , Minh T. Trần A , Takuya Wakai A and Hiroaki Funahashi A B
+ Author Affiliations
- Author Affiliations
A Department of Animal Science, Graduate School of Environmental and Life Sciences, Okayama University, Tsushima-Naka, Kita-Ku, Okayama 700-8530, Japan.
B Corresponding author. Email: hirofun@okayama-u.ac.jp
Reproduction, Fertility and Development 29(10) 1902-1909 https://doi.org/10.1071/RD16321
Submitted: 1 May 2016 Accepted: 20 October 2016 Published: 12 December 2016
Abstract
The aim of the present study was to investigate the effect of vascular endothelial growth factor (VEGF) on the meiotic and developmental competence of porcine oocytes from small follicles (SF; 0.5–3 mm diameter). When cumulus–oocyte complexes (COCs) from medium-sized follicles (MF; 3–6 mm diameter) and SF were cultured for IVM, the maturation rates were significantly higher for oocytes from MF than SF. Concentrations of VEGF in the medium were significantly higher for COCs cultured from MF than SF. When COCs from SF were exposed to 200 ng mL–1 VEGF during the first 20 h of IVM, the maturation rate improved significantly and was similar to that of oocytes derived from MF. The fertilisability of oocytes was also significantly higher than that of VEGF-free SF controls. Following parthenogenetic activation, the blastocyst formation rate improved significantly when SF COC culture was supplemented with 200 ng mL–1 VEGF, with the rate similar to that of oocytes from MF. The results of the present study indicate that VEGF markedly improves the meiotic and developmental competence of oocytes derived from SF, especially at a concentration of 200 ng mL–1 during the first 20 h of IVM.
Additional keyword: pig.
References
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