Proteomic analysis identifies potential markers in small white and small yellow follicle development in chickens
Armughan Ahmed Wadood A , Liping Pu A , Qaisar Shahzad A , Muhammad Waqas A , Lintian Yu A , Yuying Liao B , Saif ur Rehman A , Dongyang Chen A , Zhenwen Huang A and Yangqing Lu
A *
+ Author Affiliations
- Author Affiliations
A State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, College of Animal Science and Technology, Guangxi University, Nanning, Guangxi, China.
B Guangxi Veterinary Research Institute, Nanning, Guangxi, China.
Reproduction, Fertility and Development 34(6) 516-525 https://doi.org/10.1071/RD21184
Published online: 17 March 2022
© 2022 The Author(s) (or their employer(s)). Published by CSIRO Publishing
Abstract
Extensive knowledge of follicular development is imperative for improving egg production in chickens. The functional role of follicles to produce oocytes (eggs) is well recognised; however, specific markers associated with follicle development have been poorly explored. Therefore, a tandem mass tag based proteomic technique was used to identify the status of the proteome of small white follicles (1–4 mm) and small yellow follicles (6–8 mm). Analysis of differentially expressed proteins (DEP, Fold Change > 1.2, P-value < 0.05) demonstrated a total of 92 proteins (n = 92), of which 35 (n = 35) were upregulated and 57 were downregulated. DEP were further used for gene ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes pathways. The GO analysis found that DEP were mainly associated with the RNA metabolic process, cellular component organisation, peptide biosynthetic process and protein folding, thereby suggesting a key role in the follicle development process. Kyoto Encyclopedia of Genes and Genomes enrichment pathway analysis of the DEP substantiated the findings of GO analysis and described that DEP are involved in regulation of the cytoskeleton, carbon metabolism and amino acid biosynthesis. The validation of proteomic data through real-time quantitative polymerase chain reaction suggested HSPA8, HSPA2, SOD1 and FKPB3 as potential markers of small white and small yellow follicle development. This study demonstrates an understanding of proteome dynamics and represents the most comprehensive information on the entire Guangxi Ma chicken follicular proteome.
Keywords: chicken, differential gene expression, proteomics, white follicles, potential markers.
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