CSIRO Publishing blank image blank image blank image blank imageBooksblank image blank image blank image blank imageJournalsblank image blank image blank image blank imageAbout Usblank image blank image blank image blank imageShopping Cartblank image blank image blank image You are here: Journals > Reproduction, Fertility and Development   
Reproduction, Fertility and Development
Journal Banner
  Vertebrate Reproductive Science & Technology
 
blank image Search
 
blank image blank image
blank image
 
  Advanced Search
   

Journal Home
About the Journal
Editorial Board
Contacts
Content
All Issues
Special Issues
Research Fronts
Sample Issue
For Authors
General Information
Instructions to Authors
Submit Article
Open Access
For Referees
Referee Guidelines
Review an Article
Annual Referee Index
For Subscribers
Subscription Prices
Customer Service
Print Publication Dates

blue arrow e-Alerts
blank image
Subscribe to our email Early Alert or RSS feeds for the latest journal papers.

red arrow Connect with us
blank image
facebook twitter youtube

red arrow Connect with SRB
blank image
facebook TwitterIcon

Affiliated Societies

RFD is the official journal of the International Embryo Transfer Society and the Society for Reproductive Biology.


 

Article     |     Next >>   Contents Vol 22(4)

Antiperoxidative and anti-apoptotic effects of lycopene and ellagic acid on cyclophosphamide-induced testicular lipid peroxidation and apoptosis

Gaffari Türk A E, Ali Osman Çeribaşi B, Fatih Sakin C, Mustafa Sönmez A, Ahmet Ateşşahin D

A Department of Reproduction and Artificial Insemination, Faculty of Veterinary Medicine, Fırat University, 23119, Elazığ, Turkey.
B Department of Pathology, Faculty of Veterinary Medicine, Fırat University, 23119, Elazığ, Turkey.
C Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Mustafa Kemal University, 31040, Hatay, Turkey.
D Department of Pharmacology and Toxicology, Faculty of Veterinary Medicine, Fırat University, 23119, Elazığ, Turkey.
E Corresponding author. Email: gturk@firat.edu.tr
 
PDF (1.4 MB) $25
 Export Citation
 Print
  


Abstract

The present study was conducted to investigate the possible protective effects of lycopene (LC) and ellagic acid (EA) on cyclophosphamide (CP)-induced testicular and spermatozoal toxicity associated with the oxidative stress and apoptosis in male rats. Forty-eight healthy adult male Sprague-Dawley rats were divided into six groups of eight rats each. The control group was treated with placebo; the LC, EA and CP groups were given LC (10 mg kg–1), EA (2 mg kg–1) and CP (15 mg kg–1), respectively, alone; the CP+LC group was treated with a combination of CP (15 mg kg–1) and LC (10 mg kg–1); and the CP+EA group was treated with a combination of CP (15 mg kg–1) and EA (2 mg kg–1). All treatments were maintained for 8 weeks. At the end of the treatment period, bodyweight and the weight of the reproductive organs, sperm concentration and motility, testicular tissue lipid peroxidation, anti-oxidant enzyme activity and apoptosis (i.e. Bax and Bcl-2 proteins) were determined. Administration of CP resulted in significant decreases in epididymal sperm concentration and motility and significant increases in malondialdehyde levels. Although CP significantly increased the number of Bax-positive (apoptotic) cells, it had no effect on the number of Bcl-2-positive (anti-apoptotic) cells compared with the control group. However, combined treatment of rats with LC or EA in addition to CP prevented the development of CP-induced lipid peroxidation and sperm and testicular damage. In conclusion, CP-induced lipid peroxidation leads to structural and functional damage, as well as apoptosis, in spermatogenic cells of rats. Both LC and EA protect against the development of these detrimental effects.

Keywords: sperm characteristics.


   
Subscriber Login
Username:
Password:  

    
Legal & Privacy | Contact Us | Help

CSIRO

© CSIRO 1996-2014