Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
RESEARCH ARTICLE

Induction of the ovulatory LH surge in Asian elephants (Elephas maximus): a novel aid in captive breeding management of an endangered species

Chatchote Thitaram A B F , Pornsawan Pongsopawijit A , Saran Chansitthiwet C , Janine L. Brown D , Kannikar Nimtragul E , Khajhonpat Boonprasert C , Pongpon Homkong C , Sittidet Mahasawangkul C , Suvichai Rojanasthien A , Ben Colenbrander B , Gysbert C. van der Weijden B and Frank J. C. M. van Eerdenburg B
+ Author Affiliations
- Author Affiliations

A Faculty of Veterinary Medicine, Chiang Mai University, Chiang Mai, 50100, Thailand.

B Faculty of Veterinary Medicine, Utrecht University, Marburglaan 2, 3584 CN, Utrecht, The Netherlands.

C Thai Elephant Conservation Center, National Elephant Institute, Forest Industry Organisation, Lampang, 52190, Thailand.

D Conservation and Research Center, National Zoological Park, Smithsonian Institution, Front Royal,Virginia 22630, USA.

E Chiang Mai Zoo, The Zoological Park Organisation Under the Royal Patronage of H. M. The King, Chiang Mai, 50200, Thailand.

F Corresponding author. Email: thitaram@chiangmai.ac.th

Reproduction, Fertility and Development 21(5) 672-678 https://doi.org/10.1071/RD08296
Submitted: 17 December 2008  Accepted: 16 March 2009   Published: 22 May 2009

Abstract

A unique feature of the reproductive physiology of Asian elephants (Elephas maximus) is the occurrence of two LH surges before ovulation, instead of one. An anovulatory LH (anLH) surge, the function of which is unknown, occurs consistently 3 weeks before the ovulatory LH (ovLH) surge that induces ovulation. Thus, the ability to induce an ovLH surge would be useful for scheduling natural mating or artificial insemination. The present study tested the efficacy of a gonadotrophin-releasing hormone agonist (GnRH-Ag) to induce LH surges during the follicular phase of the oestrous cycle, which resulted in varied LH responses, but generally none were as high as previously documented natural surges. Thus, for the ovulation-induction trials, nine females were administered 80 μg GnRH-Ag intravenously at three time periods during the oestrous cycle, namely the anovulatory follicular phase, the ovulatory follicular phase and the luteal phase. During the late anovulatory follicular phase, nine of 10 females (90%) responded with an immediate LH surge followed 15–22 days later by an ovLH surge or a post-ovulatory increase in progestagens. In contrast, despite responding to the GnRH-Ag with an immediate increase in LH, none of the females treated during other periods of the oestrous cycle exhibited subsequent ovLH surges. One cow got pregnant from natural mating following the induced ovLH surge. In conclusion, ovLH induction is possible using a GnRH-Ag, but only during a specific time of the anovulatory follicular phase.

Additional keywords: gonadotropin-releasing hormone, ovulation induction.


Acknowledgements

The authors thank Intervet/Schering-Plough Animal Health (Thailand) Ltd, the National Research Council of Thailand, Royal Thai Government scholarship, Thailand, and the Smithsonian Women’s Committee, USA, for research funding. The authors also thank Mr Pichai Jirawattanapong, Utrecht University, for statistical assistance, Ms Ratchaneewan Khejornwong and Ms Kannikar Timhom, Chiang Mai Zoo, for endocrine laboratory assistance, Dr A. F. Parlow, NIDDK National Hormone and Pituitary Program, USA, for endocrine laboratory support, Professor Tom Stout, Utrecht University, and Dr Thomas Hildebrandt, Institute of Zoo Biology and Wildlife Research, Berlin, for scientific assistance, and Mr Worawit Rojanaphaitoon, National Elephant Institute, for allowing the study of the elephants. Finally, the authors thank all the mahouts and staff of the Thai Elephant Conservation Center.


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