Acute fasting before conception affects metabolic and endocrine status without impacting follicle and oocyte development and embryo gene expression in the rabbit
R. M. García-García A G , P. G. Rebollar B , M. Arias-Álvarez A , O. G. Sakr B F , P. Bermejo-Álvarez C , G. Brecchia D , A. Gutierrez-Adan C , M. Zerani E , C. Boiti D and P. L. Lorenzo A
+ Author Affiliations
- Author Affiliations
A Dpto. Fisiología (Fisiología Animal), Facultad de Veterinaria, Universidad Complutense de Madrid, Ciudad Universitaria, s/n, Madrid 28040, Spain.
B Dpto. Producción Animal, ETSI Agrónomos, Universidad Politécnica de Madrid, Ciudad Universitaria, s/n, Madrid 28040, Spain.
C Dpto. Produccion Animal y Conservación de Recursos Zoogenéticos, INIA, Ctra de La Coruña, Km 5.9, Madrid 28040, Spain.
D Dipartimento di Scienze Biopatologiche e Igiene delle produzioni animali e alimentari, Laboratorio di Biotecnologie fisiologiche, Sezione di Fisiologia veterinaria, Università di Perugia, Via S. Costanzo 4, I-06100, Perugia, Italy.
E Scuola di Scienze Mediche Veterinarie, Università degli Studi di Camerino, via Circonvallazione 93, I-62024 Matelica, Italy.
F Department of Animal Production, Faculty of Agriculture, Cairo University, C.P. 12613, Giza, Egypt.
G Corresponding author. Email: rosa.garcia@vet.ucm.es
Reproduction, Fertility and Development 23(6) 759-768 https://doi.org/10.1071/RD10298
Submitted: 8 November 2010 Accepted: 1 March 2011 Published: 21 June 2011
Abstract
Food deprivation affects female reproduction. The goal of the present study was to elucidate in the rabbit model the effects of acute energy restriction on ovarian function (follicle development, atresia rate and in vitro oocyte maturation) and embryonic development and gene expression of some candidate genes. Serum metabolic parameters (non-esterified fatty acids (NEFA), triglycerides, glucose, insulin and leptin concentrations) and endocrine markers (oestradiol-17β and progesterone concentrations) were also studied. A control group of nulliparous does fed ad libitum and a 72-h fasted group were used. At the end of the nutritional treatment, the ovaries of half of the animals were retrieved while the other animals were re-fed and artificially inseminated to recover embryos at 84 h after insemination, during the luteal phase. At the end of fasting, increased serum NEFA and decreased leptin concentrations were observed in the fasted group, but no differences appeared in serum steroid concentrations, follicle population and atresia rate or nuclear and cytoplasmic oocyte maturation. In the luteal phase, insulin concentrations increased notably in the fasted group. The number of recovered embryos per female and the speed of embryo development were reduced in the food-deprived group. Acute fasting altered both metabolic and endocrine markers and embryo development, but follicle and oocyte development and embryo gene expression were not affected.
Additional keywords: apoptosis, maternal–embryo interaction.
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