Apoptosis signal-regulating kinase (ASK-1) controls ovarian cell functions
Alexander V. Sirotkin A B E , Andrej Benčo A , Jan Kotwica C , Saleh H. Alwasel D and Abdel H. Harrath D
+ Author Affiliations
- Author Affiliations
A Constantine the Philosopher University, 949 74 Nitra, Slovakia.
B Research Institute of Animal Production, National Agricultural and Food Centre, 951 41 Lužianky, Slovakia.
C Institute of Animal Reproduction and Food Research, 10-718 Olsztyn-Kortowo, Poland.
D Department of Zoology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
E Corresponding author. Email: asirotkin@ukf.sk
Reproduction, Fertility and Development 31(11) 1657-1664 https://doi.org/10.1071/RD19055
Submitted: 11 February 2019 Accepted: 29 May 2019 Published: 20 June 2019
Abstract
The involvement of the apoptosis signal-regulating kinase 1 (ASK1)-related signalling pathway in the control of reproduction is unknown. This study aimed to investigate the role of ASK-1 in the control of basic ovarian functions (proliferation, apoptosis and hormone release) and its response to ovarian hormonal regulators (leptin and FSH). We compared the accumulation of ASK-1, proliferation marker proliferating cell nuclear antigen (PCNA), apoptosis marker Bax and apoptosis and proliferation regulating transcription factor p53 and the release of progesterone (P4), oxytocin (OT), insulin-like growth factor I (IGF-I) and prostaglandins F (PGF) and E (PGE) using cultured porcine ovarian granulosa cells transfected with ASK-1 cDNA and cultured with leptin or FSH. This study is the first to demonstrate that ASK-1 does not affect cell apoptosis and viability in ovarian cells, but promotes cell proliferation, suppresses p53, alters the release of ovarian hormones (P4, OT, IGF-I, PGF and PGE) and defines their response to the upstream hormonal regulators leptin and FSH. Therefore, ASK-1 can be considered a new and important regulator of multiple ovarian functions.
Additional keywords: hormones, ovarian granulosa cells, pig, proliferation, transfection.
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