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RESEARCH ARTICLE

The phytoestrogen, quercetin, in serum, uterus and ovary as a potential treatment for dehydroepiandrosterone-induced polycystic ovary syndrome in the rat

Majid Jafari Khorchani A , Fatemeh Zal https://orcid.org/0000-0002-7078-4854 A B C and Asma Neisy A
+ Author Affiliations
- Author Affiliations

A Biochemistry Department, School of Medicine, Shiraz University of Medical Sciences, Shiraz, 7134845794, Iran.

B Infertility Research Center, Shiraz University of Medical Sciences, Shiraz 7134845794, Iran.

C Corresponding author. Email: fatemehzal@yahoo.com

Reproduction, Fertility and Development 32(3) 313-321 https://doi.org/10.1071/RD19072
Submitted: 12 January 2019  Accepted: 1 July 2019   Published: 30 October 2019

Abstract

The exact mechanisms of polycystic ovary syndrome (PCOS) are unknown and there is no effective cure for the disease. The aim of this study was to evaluate the alterations in serum oestradiol and adiponectin levels and in the expression of some important genes in the uterine and ovarian tissues of PCOS rats. The therapeutic effect of quercetin on PCOS was also assessed. Rats were divided into five groups: control, ethanol, quercetin (Q), PCOS and PCOS+Q. After 30 days of oral treatments, the rats’ ovaries and uteri were removed and nesfatin-1, aromatase and adipoR1 expressions were quantified with real-time polymerase chain reaction. Serum adiponectin and oestradiol levels were evaluated using enzyme-linked immunosorbent assay technique. The results of this study showed that expression of nesfatin-1 and adipoR1 genes and adiponectin serum levels decreased in the PCOS rats, but aromatase expression and oestradiol level increased. Treatment with quercetin increased the adiponectin level and expression of adipoR1 and nesfatin-1 and decreased both the expression of aromatase and the oestradiol level. Quercetin improved PCOS by phytoestrogenic effects and mimicking oestrogen’s function. Quercetin also affects important factors in both the uterus and ovary and could improve the obesity and the diabetic and infertility symptoms of PCOS.

Additional keywords: adipoR1, aromatase, nesfatin-1, oestradiol.


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