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Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
Reproduction, Fertility and Development

Reproduction, Fertility and Development

Volume 34 Number 13 2022

RD22115Reproductive biology research down under: highlights from the Australian and New Zealand Annual Meeting of the Society for Reproductive Biology, 2021

Jessica E. M. Dunleavy 0000-0003-2009-0165, Doan Thao Dinh, Caitlin E. Filby, Ella Green 0000-0002-3009-3442, Pierre Hofstee 0000-0002-4716-4016, Taylor Pini 0000-0003-4272-9943, Nicola Rivers 0000-0003-0533-3480, David A. Skerrett-Byrne 0000-0002-1804-1826, Rukmali Wijayarathna 0000-0002-5584-1371, Yasmyn E. Winstanley, Wei Zhou and Dulama Richani
pp. 855-866
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The Society for Reproductive Biology (SRB) is an Australian and New Zealand based organisation that supports basic and applied research across all aspects of reproductive biology. This review highlights the key themes from the annual SRB meeting, which was held virtually in 2021. Topics included novel technologies in reproduction, advances in contraception, non-model species, reproductive ageing and sustaining a pregnancy.

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Amphibian biodiversity is rapidly declining and we must improve our ability to captive breed animals. However, artificial reproductive techniques are often species specific and different species require specific protocols. We tested two hormone treatments on sperm induction in an endangered frog and found that only one hormone was successful, and sperm quantity and quality decreased rapidly after treatment. This study represents a useful starting point for developing artificial reproductive techniques for non-model organisms to improve captive breeding strategies.

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With only two northern white rhinos left in the world, it is necessary to study oocyte and embryo development in a model subspecies, the southern white rhino. This study evaluated gene expression in the granulosa cells responsible for oocyte maturation, as well as oocyte glucose uptake during in vitro maturation. This information is crucial to optimise in vitro maturation and embryo development for the southern, and ultimately the northern, white rhino.

RD22130Vitrification of bovine germinal vesicle oocytes significantly decreased the methylation level of their in vitro derived MII oocytes

Peipei Zhang, Sha Yang, Hang Zhang, Haisheng Hao, Weihua Du, Jingjing Wang, Tong Hao, Huabin Zhu, Saqib Umer 0000-0001-6110-2690 and Xueming Zhao
pp. 889-903
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5-MeC and SC-WGBS were utilised to detect the DNA methylation patterns of vitrified bovine GV oocytes and their in vitro derived MII oocytes. Results showed that the methylation level of the V_GV group was similar to that of the F_GV group, while the methylation level of the V_MII group was significantly lower than that of the F_MII group. Moreover, supplementation of 2 μM Res in IVM medium significantly improved maturation and development ability of vitrified GV oocytes by restoring their DNA methylation levels.

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Reproduction, Fertility and Development is now publishing technical reports, i.e. significant technical advances. More

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