46,XY, disorders of sexual development (46,XY, DSD) is a congenital genetic disease. The existing molecular research has focused on single-centre sequencing data, instead of prediction based on big data. In this study, the relevant pathogenic genes are predicted by bioinformatics, and verified by gene sequencing technology. Our results indicate that occurrence of 46,XY, DSD is attributed to a variety of genes. Bioinformatics analysis can help us predict the hub genes and find the most core network MCODE model.

This is a way to improve the in vitro production of pig embryos, which has low efficiency. It consists of supplementing the gamete culture media with two antioxidants, metformin and ITS (insulin–transferrin–selenium), since one of the biggest problems in in vitro production is oxidative stress. This is the first study on the effects of these supplements and shows promising results that could be used even in other animal models, or even in human ART.