Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
Reproduction, Fertility and Development

Reproduction, Fertility and Development

Volume 24 Number 5 2012

RD11167Increasing expression of oxytocin and vasopressin receptors in the equine conceptus between Days 10 and 16 of pregnancy

Sven Budik, Franziska Palm, Ingrid Walter, Magdalena Helmreich and Christine Aurich
pp. 641-648

Oxytocin and vasopressin are present in the yolk sac of the equine pre-attachment embryo. They are supposable candidates for the regulation of equine embryo expansion mediated by aquaporins and ATPases abundant in the trophoblast. Expression of oxytocin, vasopressin 1A and 2 receptors could be proven by means of RT-PCR and immunohistochemistry in equine embryos between Days 10 and 16, making involvement of these two hormones very probable for embryo expansion.

RD11174Effects of anthocyanin extracted from black soybean seed coat on spermatogenesis in a rat varicocele-induced model

Hoon Jang, Su Jin Kim, Seung Mo Yuk, Dong Seok Han, U.-Syn Ha, Sung Hoo Hong, Ji Yeol Lee, Tae Kon Hwang, Seong Yeon Hwang and Sae Woong Kim
pp. 649-655

Until now the only possible treatment for varicocele, the most common cause of primary male infertility, has been surgery. In the present study, anthocyanin improved sperm motility, spermatogenic cell density and testis weight. These actions are likely due to anthocyanin-induced reductions in oxidative damage resulting from varicocele-induced venous stasis. The results of the present study may be the initial step in developing a medical approach for the treatment of varicocele.

RD11118Paradoxical effects of kisspeptin: it enhances oocyte in vitro maturation but has an adverse impact on hatched blastocysts during in vitro culture

Islam M. Saadeldin, Ok Jae Koo, Jung Taek Kang, Dae Kee Kwon, Sol Ji Park, Su Jin Kim, Joon Ho Moon, Hyun Ju Oh, Goo Jang and Byeong Chun Lee
pp. 656-668

This is the first report showing the existence and interaction of kisspeptin and its receptors in porcine cumulus–oocyte complexes and its enhancing role in oocyte maturation and subsequent embryo developmental competence in an autocrine–paracrine manner. However, Kp supplementation may have a paradoxical impact on hatched blastocyst development reflecting systemic or local regulation during this early critical period.

RD11229Evaluation of maize grain and polyunsaturated fatty acid (PUFA) as energy sources for breeding rams based on hormonal, sperm functional parameters and fertility

Sellappan Selvaraju, Priyadarshini Raju, Somu Bala Nageswara Rao, Subbarao Raghavendra, Sumantha Nandi, Dhanasekaran Dineshkumar, Allen Thayakumar, Shivashanmugam Parthipan and Janivara Parameswaraiah Ravindra
pp. 669-678

The fatty acid composition of supplemented fat in the diet has a role in improving reproductive efficiency. The study was carried out to elucidate the effect of different sources of dietary energy (maize vs polyunsaturated fatty acid (PUFA)-rich sunflower oil) on sperm quality in rams and the result suggested that dietary PUFA protected sperm membrane integrity. Developing a dietary regimen that includes PUFA might improve the fertility potential of bulls.


Oocyte maturation is critical to its developmental potential and is characterized by changes in the distribution and activity of mitochondria in the oocyte. We investigated the effect of two fat energy sources, linoleic acid (LA; omega-6) and alpha-linolenic acid (ALA; omega-3), on bovine oocyte maturation and showed negative effects of LA and positive effects of ALA on the mitochondria profile. High LA levels in diets may be detrimental for oocyte development and result in reduced fertility.

RD11068Gene expression profiling of pluripotency and differentiation-related markers in cat oocytes and preimplantation embryos

Muriel Filliers, Karen Goossens, Ann Van Soom, Barbara Merlo, Charles Earle Pope, Hilde de Rooster, Katrien Smits, Leen Vandaele and Luc J. Peelman
pp. 691-703

A basic understanding of the molecular pathways that regulate developmental events during early feline embryogenesis is still lacking. The aim of the study was to quantify mRNA profiles of pluripotency and differentiation-related markers during preimplantation development and results showed that these genes are expressed and undergo species-specific changes during early embryonic development. These findings add evidence to the hypothesis that behavior of these markers may differ among species.

RD11160The inhibitory effect of progesterone on lactogenesis during pregnancy is already evident by mid- to late gestation in rodents

Constanza M. López-Fontana, María E. Maselli, Ana M. Salicioni and Rubén W. Carón
pp. 704-714

Mammary gland development during pregnancy is a tightly regulated process necessary for lactation, and strongly dependent on hormonal control. This report aimed to investigate whether the same hormonal changes that lead to parturition in rats were responsible for triggering mammary gland maturation required for the initiation of milk production. Our study provides clear in vivo evidence of the role of progesterone as a key hormonal player for both mammary development and milk production, and demonstrates that lactogenesis may be advanced as long as the action of progesterone is abolished.

RD11246Alterations in systemic concentrations of progesterone during the early luteal phase affect RBP4 expression in the bovine uterus

Michael P. Mullen, Niamh Forde, Mervyn H. Parr, Michael G. Diskin, Dermot G. Morris, Jarlath E. Nally, Alexander C. O. Evans and Mark A. Crowe
pp. 715-722

Embryo survival is a major factor affecting reproductive success with both systemic progesterone and retinol demonstrating significant effects on embryo development. Our aim was to determine the effects of systemic progesterone on the uterine expression of retinol’s transportation protein, RBP4, in cyclic dairy cattle. RBP4 expression in the bovine uterus was altered by progesterone in a time- and concentration-dependant manner and therefore may play a role in regulating embryo development in cattle.

RD11195Levels of mRNA for bone morphogenetic proteins, their receptors and SMADs in goat ovarian follicles grown in vivo and in vitro

J. J. N. Costa, M. J. Passos, C. C. F. Leitão, G. L. Vasconcelos, M. V. A. Saraiva, J. R. Figueiredo, R. van den Hurk and J. R. V. Silva
pp. 723-732

This study brings new information on bone morphogenetic proteins (BMPs) that can contribute to the understanding of ovarian folliculogenesis. The levels of mRNA for BMP system in goat preantral follicles before and after culture for 18 days were evaluated. Increased levels of mRNA for BMP-2 and reduced mRNA for BMP-4, BMP-7, BMPR-IA and SMAD-5 were observed in cultured follicles. These data are essential for improvement of in vitro development of good-quality oocytes.

RD10301Acute fasting decreases the expression of GLUT1 and glucose utilisation involved in mouse oocyte maturation and cumulus cell expansion

Yingying Han, Jun Yan, Jinlian Zhou, Zhen Teng, Fenghua Bian, Meng Guo, Guankun Mao, Junxia Li, Jianwei Wang, Meijia Zhang and Guoliang Xia
pp. 733-742

Undernutrition as a result of dietary restriction can contribute to female infertility. In this study, we examine the effects of undernutrition on oocyte development and demonstrate that this process is dependent on GLUT1 expression and glucose uptake in cumulus-oocyte complexes. These results demonstrate the importance of proper nutrition and glucose metabolism in female fertility and implicate GLUT1 in this process.


This study assessed the effects of follicle fluid on fertilisation ability of bovine oocytes. Supplementation of culture medium with follicle fluid during in vitro maturation of oocytes promoted sperm penetration during in vitro fertilisation both by improving cumulus expansion and by enhancing ATP levels in oocytes, which maintained their ability to be fertilised even after mitochondrial stress. This finding may be useful for further development of maturation media especially when oocytes are marked out for mitochondria-damaging treatments.

RD11143Antenatal glucocorticoids reduce growth in appropriately grown and growth-restricted ovine fetuses in a sex-specific manner

Suzanne L. Miller, Amy E. Sutherland, Veena G. Supramaniam, David W. Walker, Graham Jenkin and Euan M. Wallace
pp. 753-758

Antenatal glucocorticoids are administered to pregnant women in preterm labour and enhance survival of preterm infants by maturing the lungs prior to birth. However, these synthetic glucocorticoids may reduce normal patterns of fetal growth, with this study showing that this is significantly pronounced in female fetuses. These data suggest that the fetus and/or placenta of male and female fetuses may differentially regulate the response to maternal glucocorticoids.

RD11164Generation of myometrium-specific Bmal1 knockout mice for parturition analysis

Christine K. Ratajczak, Minoru Asada, Gregg C. Allen, Douglas G. McMahon, Lisa M. Muglia, Donté Smith, Sandip Bhattacharyya and Louis J. Muglia
pp. 759-767

Previous studies indicate that the genes that control circadian rhythmicity also have a role in parturition. A transgenic mouse was generated in which the rhythmicity-associated gene Bmal1 is deleted in the myometrium; females of this transgenic line were less likely to labour at the expected time of day than control mice. This report introduces a new transgenic mouse model and suggests an important role for myometrium-specific expression of Bmal1 in parturition timing.

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