Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
Table of Contents
Reproduction, Fertility and Development

Reproduction, Fertility and Development

Volume 25 Number 8 2013

RD12275Effect of varying glucose and glucosamine concentration in vitro on mouse oocyte maturation and developmental competence

L. A. Frank, M. L. Sutton-McDowall, D. L. Russell, X. Wang, D. K. Feil, R. B. Gilchrist and J. G. Thompson
pp. 1095-1104

Glucose flux through metabolic pathways during cumulus–oocyte maturation plays a crucial role in determining oocyte developmental competence, both in vitro and in vivo. In these experiments we examined the possible roles of the various pathways during mouse oocyte in vitro maturation (IVM) with an emphasis on the hexosamine biosynthesis pathway. Interestingly, glucose or glucosamine supplementation during the first hour of IVM was essential for further development.

RD12253Effects of systemic administration or intrabursal injection of serotonin on puberty, first ovulation and follicular development in rats

M. J. Moran, M. E. Ayala, E. Gallegos, J. Romero, R. Chavira, P. Damián-Matsumura and R. Domínguez
pp. 1105-1114

The serotonin regulates gonadotrophin secretion. The aim of this study was to analyze the effects of an increase of both systemic and in-ovarian bursa of serotonin on onset of puberty and ovarian function. We proposed that serotonin participates in the modulation of the neuroendocrine mechanisms that regulate the hypothalamus–hypophisis–ovary axis and culminates with the regulation of follicular growth, ovulation, and steroidogenesis. Also that serotonin acts at the ovary, directly modulating steroidogenesis.

RD12187Interplay between autophagy and apoptosis in the development of Danio rerio follicles and the effects of a probiotic

Giorgia Gioacchini, Luisa Dalla Valle, Francesca Benato, Gian Maria Fimia, Roberta Nardacci, Fabiola Ciccosanti, Mauro Piacentini, Andrea Borini and Oliana Carnevali
pp. 1115-1125

The autophagic process was detected in preovulatory follicles isolated from Danio rerio. Modulation of apoptotic and autophagic processes was demonstrated in vitellogenic follicles from females fed a probiotic (Lactobacillus rhamnosus IMC 501). The results show a positive effect of L. rhamnosus on ovarian physiology, suggesting that probiotics may be developed as a new biotechnology to improve reproduction.

RD12103Identification of alpha-1 acid glycoprotein (AGP) as a potential marker of impaired growth in the newborn piglet

Thomas J. Caperna, Amy E. Shannon, Le Ann Blomberg, Margo Stoll and Timothy G. Ramsay
pp. 1126-1133

Piglets with low initial growth rates remain poor growth performers throughout the post-weaning period, which increases herd variability and reduces overall production efficiency. Blood was collected at birth and higher levels of alpha-1 acid glycoprotein, the predominant protein in fetal piglet plasma, were associated with runts and poor growing pigs. Availability of a biomarker that is reflective of in utero development or fetal maturation may serve as an early predictor of overall growth potential.


Anurans exhibit a greater reproductive diversity than any other vertebrate order. However, studies investigating optimal conditions for artificial fertilisation are limited to aquatic-breeding species. The present study investigated the effects of fertilisation medium osmolality, sperm concentration and short-term oocyte storage on fertilisation success in a terrestrial-breeding anuran, Günther’s toadlet. Optimal sperm concentrations were lower, and optimal fertilisation media osmolalities both higher and broader, than those previously reported for aquatic species.

RD12256Successful cloning of coyotes through interspecies somatic cell nuclear transfer using domestic dog oocytes

Insung Hwang, Yeon Woo Jeong, Joung Joo Kim, Hyo Jeong Lee, Mina Kang, Kang Bae Park, Jung Hwan Park, Yeun Wook Kim, Woo Tae Kim, Taeyoung Shin, Sang Hwan Hyun, Eui-Bae Jeung and Woo Suk Hwang
pp. 1142-1148

In this research, coyotes were successfully cloned using domestic dog oocytes and surrogates. The aim was to examine whether domestic dog oocytes and surrogates can be used to give birth to a different species using interspecies somatic cell nuclear transfer (iSCNT) technology. This success has provided the promise of utilising similar techniques to clone endangered canids in the future, subsequently leading to preservation of nature's diversity.

RD12330Effects of glial cell line-derived neurotrophic factor, fibroblast growth factor 2 and epidermal growth factor on proliferation and the expression of some genes in buffalo (Bubalus bubalis) spermatogonial cells

Prashant H. Kadam, Sushila Kala, Himanshu Agrawal, Karn P. Singh, Manoj K. Singh, Manmohan S. Chauhan, Prabhat Palta, Suresh K. Singla and Radhay S. Manik
pp. 1149-1157

Spermatogonial stem cells are at the core of male fertility, undergoing self-renewal and differentiation. The mechanisms underlying these processes are yet to be determined. The aim of the present study was to determine the effects of factors crucial in the testis microenvironment on the short-term culture of buffalo spermatogonia. Supplementation of the in vitro culture medium with glial cell line-derived neurotrophic factor, fibroblast growth factor 2 and epidermal growth factor had beneficial effects on the self-renewal and proliferation of spermatogonial cell colonies and their use appears suitable for short-term cultures of buffalo spermatogonia.


The oxygen environment of the oocyte is crucial to its quality and is largely determined by the oxygen consumption of the cells surrounding it. This work aimed to measure the oxygen consumption rate of such ovarian cells in cows and subsequently showed using mathematical modelling that early-stage oocytes may experience oxygen starvation. These results may be key to understanding oocyte development and optimising in vitro culture conditions.


Elephants have the longest reproductive lifespan of land-based mammals; therefore, it is of interest to study aspects of their ovarian biological clock. The numbers of ‘eggs’ present were counted in individuals between puberty and reproductive senescence to reflect this ovarian activity. The results provide an interesting comparison with the human ovarian clock and also give a base study against which the egg reserve of relatively infertile captive elephants may be compared.

RD12178Exposure to mono-n-butyl phthalate disrupts the development of preimplantation embryos

Da-Peng Chu, Shi Tian, Da-Guang Sun, Chan-Juan Hao, Hong-Fei Xia and Xu Ma
pp. 1174-1184

The widespread use of plastics provides us cheaper, lighter and more durable products, but what are the effects on human reproduction? The present study reveals that that mono-n-butyl phthalate, a metabolite of a solvent used in the production of plastics, impairs embryo developmental potency and cause a series of intracellular biochemical changes. The results highlight the need to pay more attention to the effects of environment pollutants on human reproductive health.

RD12181The antioxidant effects of soybean lecithin- or low-density lipoprotein-based extenders for the cryopreservation of brown-bear (Ursus arctos) spermatozoa

M. Alvarez-Rodríguez, M. Alvarez, L. Anel-López, C. Martínez-Rodríguez, F. Martínez-Pastor, S. Borragan, L. Anel and P. de Paz
pp. 1185-1193

Egg yolk protects brown-bear spermatozoa during cryopreservation but has some disadvantages and thus we propose to replace it by another component with similar activity. Low-density lipoproteins (LDL) and soybean lecithin were evaluated and we observed that the LDL-based extender exhibited a high anti-oxidant capacity and protection of the viability of frozen spermatozoa. We propose that LDL could be a useful substitute for egg yolk in these extenders.

RD12173Effects of growth differentiation factor-9 and FSH on in vitro development, viability and mRNA expression in bovine preantral follicles

G. L. Vasconcelos, M. V. A. Saraiva, J. J. N. Costa, M. J. Passos, A. W. B. Silva, R. O. D. S. Rossi, A. M. L. R. Portela, A. B. G. Duarte, D. M. Magalhães-Padilha, C. C. Campelo, J. R. Figueiredo, R. van den Hurk and J. R. V. Silva
pp. 1194-1203

The present study provides new information to increase our understanding of bovine ovarian folliculogenesis. During the in vitro growth of preantral follicles, FSH and/or growth differentiation factor (GDF)-9 promote follicular growth and antrum formation. Moreover, GDF-9 stimulates the expression of versican and perlecan mRNA, and interacts positively with FSH to increase the expression of HAS2. These data are essential for improving the in vitro development of good-quality oocytes.

RD12215Production of good-quality blastocyst embryos following IVF of ovine oocytes vitrified at the germinal vesicle stage using a cryoloop

Adel R. Moawad, Jie Zhu, Inchul Choi, Dasari Amarnath, Wenchao Chen and Keith H. S. Campbell
pp. 1204-1215

Oocyte cryopreservation is important for assisted reproductive technologies (ART) in both human and animals. This study showed that ovine oocytes vitrified at GV stages using cryoloop have the ability to be matured, fertilised and subsequently develop in vitro and produce good quality blastocysts at comparable trends like those seen in fresh control groups. The vitrification protocols used here may be a promising tool for the establishment of oocyte cryobanks.

RD12329Oocyte maturation and embryo survival in nulliparous female pigs (gilts) is improved by feeding a lupin-based high-fibre diet

A. C. Weaver, J. M. Kelly, K. L. Kind, K. L. Gatford, D. J. Kennaway, P. J. Herde and W. H. E. J. van Wettere
pp. 1216-1223

Inclusion of the high-fibre ingredient sugar-beet pulp in pig pre-mating diets has been shown to improve oocyte quality and embryo survival; however, it is not a commercially viable feed ingredient in Australia. This study evaluated the effects of two alternative fibre sources (lupin and bran) and found that lupin-fed pigs had improved oocyte maturation and embryo survival compared with control and bran-fed pigs. These findings could potentially be implemented as a strategy to improve litter sizes in commercial piggeries in Australia.

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