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The role of activin C in normal ovaries and granulosa cell tumours of mice and humans
Activins and inhibins play important roles in the development, growth and function of the ovary. Mice lacking inhibin develop granulosa cell tumours in their ovaries which secrete activin A and these tumours are modulated by increased activin C expression. The aim of this study was to identify where activin C is expressed in mouse and human ovaries and whether overexpression of activin C modulates normal follicular development in mice. Immunohistochemical staining for the activin-βC subunit was performed on sections from mouse and human ovaries and human adult granulosa cell tumours. Stereology techniques were used to quantify oocyte and follicular diameters, and the percentage of different follicular types in ovaries from wild-type mice and those under-expressing inhibin-α, and/or over-expressing activin C. Staining for activin-βC was observed in the oocytes, granulosa cells, thecal cells and surface epithelium of mouse and human ovaries and in the granulosa-like cells of adult granulosa cell tumours. Overexpression of activin C in mice did not alter follicular development when compared to wild-type mice but it did modulate the development of abnormal early stage follicles in inhibin-α null mice. These results provide further evidence of a role for activin C in the ovary.
RD17250 Accepted 08 November 2017
© CSIRO 2017