Australian Systematic Botany Australian Systematic Botany Society
Taxonomy, biogeography and evolution of plants
RESEARCH ARTICLE

Phylogeny and classification of Eucalyptus subgenus Eudesmia (Myrtaceae) based on nuclear ribosomal DNA, chloroplast DNA and morphology

Adele K. Gibbs A , Frank Udovicic B , Andrew N. Drinnan A and Pauline Y. Ladiges A C
+ Author Affiliations
- Author Affiliations

A School of Botany, The University of Melbourne, Vic. 3010, Australia.

B Royal Botanic Gardens Melbourne, Birdwood Avenue, South Yarra, Vic. 3141, Australia.

C Corresponding author. Email: p.ladiges@unimelb.edu.au

Australian Systematic Botany 22(3) 158-179 https://doi.org/10.1071/SB08043
Submitted: 11 September 2008  Accepted: 24 February 2009   Published: 10 June 2009

Abstract

Phylogenetic analysis of Eucalyptus subgenus Eudesmia is presented on the basis of the following three datasets: sequences of the internal transcribed spacer (ITS) and the external transcribed spacer (ETS) regions from nuclear rDNA, sequences of the psbA–trnH intergenic spacer region from chloroplast DNA, and morphological characters, including stamen bundling, operculum development, seeds and trichomes. Studies of floral development were essential for understanding the morphology of mature flowers and interpretation of synapomorphy and homoplasy. A summary phylogeny was constructed from a maximum parsimony analysis of those nodes coded as characters that had support in the molecular trees together with morphological characters. A revised infra-subgeneric classification is presented on the basis of the summary phylogeny, and compared with classifications of Hill and Johnson (1998) and Brooker (2000). Differences relate to relationships between clades and taxonomic rank (sections, series and subseries) and valid names of Brooker (2000) are conserved where possible. One main clade of 14 species (section Limbatae), many of mallee growth form, was found in all analyses; this clade is distributed in the South-West of Western Australia and adjacent Interzone and desert areas. A second main clade (section Complanatae) occurs in the northern and eastern tropical and subtropical regions of Australia, including Kimberley, Arnhem, Queensland and New South Wales. This section includes E. tetrodonta, previously treated as an isolated taxon in a monotypic section; however, this species is related to E. baileyana, E. similis, E. lirata and series Miniatae. The hypothesised phylogeny provides a framework for further analyses of biogeography and ecology, including functional traits.


Acknowledgements

Dean Nicolle, Marjorie King, Royal Botanic Gardens Sydney Seedbank, and Kings Park and Botanic Gardens provided plant material and seeds. Wayne Gebert and Neville Walsh provided Latin diagnoses. This work was funded by ARC Linkage Grant LP0455375, with support from Royal Botanic Gardens Melbourne, and Maud Gibson Trust. AG was supported by the Hansjörg Eichler Scientific Research Award (Australian Systematic Botany Society), a Melbourne Research Scholarship, the Ethel McLennan Award (The Botany Foundation) and an Albert Shimmin Award, Faculty of Science, University of Melbourne.


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Appendix 1.  DNA sources or published sequences used in analyses (shown here in bold), wet collections of flower buds and examples of voucher specimens examined for morphology
AB, A. Bohte; AD, Adelaide Herbarium; AG, A. Gibbs; AND, A. N. Drinnan; CANB, Canberra Herbarium; CCA, Currency Creek Arboretum, South Australia; CL, Cookson Laboratory, The University of Melbourne; CSIRO, Commonwealth Scientific and Industrial Research Organisation, Australian Tree Seed Centre; FRI, CSIRO Division of Forest Research, Yarralumla; KPBG, Kings Park and Botanic Gardens, Perth; MELU, The University of Melbourne Herbarium; MIHB, M. I. H. Brooker; NSW, New South Wales; NT, Northern Territory; PYL, P. Y. Ladiges; Qld, Queensland; RBG, Syd., Royal Botanic Gardens, Sydney; SA, South Australia; Vic., Victoria; WA, Western Australia. Subgenera are according to Brooker (2000). For the Currency Creek material, vouchers numbers (DN#) are those of D. Nicolle and are for the parent tree from which first generation offspring were grown (see Nicolle 2003)
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