Australian Systematic Botany Australian Systematic Botany Society
Taxonomy, biogeography and evolution of plants
RESEARCH ARTICLE

A molecular phylogeny and infrageneric classification for Kunzea (Myrtaceae) inferred from rDNA ITS and ETS sequences

P. J. de Lange A E , R. D. Smissen B , S. J. Wagstaff B , D. J. Keeling C , B. G. Murray C and H. R. Toelken D
+ Author Affiliations
- Author Affiliations

A Ecosystems and Species Unit, Department of Conservation, Private Bag 68908, Newton 1145, Auckland, New Zealand.

B Allan Herbarium, Landcare Research, PO Box 40, Lincoln 7640, New Zealand.

C School of Biological Sciences, University of Auckland, Private Bag 92019, Auckland Mail Centre, Auckland 1142, New Zealand.

D State Herbarium of South Australia, PO Box 2732, Kent Town, SA 5071, Australia.

E Corresponding author. Email: pdelange@doc.govt.nz

Australian Systematic Botany 23(5) 309-319 https://doi.org/10.1071/SB10019
Submitted: 20 May 2010  Accepted: 9 September 2010   Published: 3 November 2010

Abstract

Circumscription and infrageneric classification of Kunzea is evaluated after phylogenetic analyses of nrDNA ITS and ETS sequences. Kunzea is monophyletic if the K. ericoides complex and the monotypic Angasomyrtus are included. The genus can be divided into western Australian, eastern Australian, and eastern Australian plus New Zealand clades. Angasomyrtus has greater affinity with the eastern Kunzea species, despite its western Australian endemic status. A new infrageneric classification is proposed, recognising four subgenera, with a further division into sections within two of these. A new combination in Kunzea is made for Angasomyrtus.


References

Andrews JRH (1986) The Southern Ark – zoological discovery in New Zealand 1769–1900. (University of Hawai’I Press: Honolulu, Hawai’i)

Baldwin BG, Markos S (1998) Phylogenetic utility of the external transcribed spacer (ETS) of the 18S–26S rDNA: congruence of ETS and ITS trees of Calycadenia (Compositae). Molecular Phylogenetics and Evolution 10, 449–463.
Phylogenetic utility of the external transcribed spacer (ETS) of the 18S–26S rDNA: congruence of ETS and ITS trees of Calycadenia (Compositae).CrossRef | 1:CAS:528:DyaK1MXhtlKmtb4%3D&md5=6626f032802d31e874258514f3b1ab86CAS | 10051397PubMed |

Beardsell DV, O’Brien SP, Williams EG, Knox RB, Calder DM (1993) Reproductive biology of Australian Myrtaceae. Australian Journal of Botany 41, 511–526.
Reproductive biology of Australian Myrtaceae.CrossRef |

Benson D, McDougall L (1998) Ecology of Sydney plant species. Part 6. Dicotyledone family Myrtaceae. Cunninghamia 5, 808–987.

Bentham G (1867) Kunzea. In ‘Flora Australiensis. Vol. 3’. pp. 111–118. (Reeve: London).

Briggs BG, Johnson LAS (1979) Evolution of the Myrtaceae – evidence from inflorescence structure. Proceedings of the Linnean Society of New South Wales 102, 157–256.

Brown GK, Udovicic F, Ladiges PY (2001) Molecular phylogeny and biogeography of Melaleuca, Callistemon and related genera (Myrtaceae). Australian Systematic Botany 14, 565–585.
Molecular phylogeny and biogeography of Melaleuca, Callistemon and related genera (Myrtaceae).CrossRef |

Clevinger JA, Panero JL (2000) Phylogenetic analysis of Silphium and subtribe Engelmanniinae (Asteraceae: Heliantheae) based on its ITS and ETS sequence data. American Journal of Botany 87, 565–572.
Phylogenetic analysis of Silphium and subtribe Engelmanniinae (Asteraceae: Heliantheae) based on its ITS and ETS sequence data.CrossRef | 1:CAS:528:DC%2BD3cXjsVektrc%3D&md5=6a961f95420b12fdac9a3f664bc110b8CAS | 10766728PubMed |

Dawson JW, Whitaker AH, Whitaker VA, Gardner RC, Wright SD (2000) Two new species of Metrosideros (Myrtaceae) from New Caledonia: dual characterisation with morphology and nrDNA sequence variation. Blumea 45, 433–441.

de Lange PJ (2006a) Why Kunzea? Why not Leptospermum? New Zealand Botanical Society Newsletter 85, 9–11.

de Lange PJ (2006b) Kunzea. In ‘AL Eagle’s complete trees and shrubs of New Zealand. Vol. 1’. (Ed. AL Eagle) pp. 234–239. (Te Papa Press: Wellington, New Zealand)

de Lange PJ (2007a) Biosystematics of the New Zealand Kunzea ericoides (A.Rich.) Joy Thomps. complex. PhD Thesis, University of Auckland.

de Lange PJ (2007b) Biosystematics of the Kunzea ericoides (A.Rich.) Joy Thomps. (Myrtaceae) complex. Conference proceedings: abstracts of papers presented at the joint T. F. Cheeseman symposium and New Zealand Plant Conservation Network annual conference. New Zealand Journal of Botany 45, 290

de Lange PJ, Murray BG (2004) Chromosome numbers in Kunzea (Myrtaceae). Australian Journal of Botany 52, 609–617.
Chromosome numbers in Kunzea (Myrtaceae).CrossRef | 1:CAS:528:DC%2BD2cXptVejt7s%3D&md5=3877f94b6f5dd4147f54002f8003d4ceCAS |

de Lange PJ, Datson PM, Murray BG, Toelken HR (2005) Hybridism in the Kunzea ericoides complex (Myrtaceae): an analysis of artificial crosses. Australian Systematic Botany 18, 117–131.
Hybridism in the Kunzea ericoides complex (Myrtaceae): an analysis of artificial crosses.CrossRef |

Farris JS, Källersjö M, Kuge AG, Bult C (1994) Testing significance of incongruence. Cladistics 10, 315–319.
Testing significance of incongruence.CrossRef |

Farris JS, Källersjö M, Kuge AG, Bult C (1995) Constructing a significance test for incongruence. Systematic Biology 44, 570–572.

Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39, 783–791.
Confidence limits on phylogenies: an approach using the bootstrap.CrossRef |

Giegerich R, Meyer F, Schleiermacher C (1996) GeneFisher – Software support for the detection of postulated genes. In ‘Proceedings of the 4th international conference on intelligent systems for molecular biology’. (Eds DJ States, P Agarwal, T Gaasterland, L Hunter, RF Smith) pp. 68–77. (AAAI Press: St Louis, MO)

Harrington MG, Gadek PA (2004) Molecular systematics of the Acmena alliance (Myrtaceae): phylogenetic analyses and evolutionary implications with reference to Australian taxa. Australian Systematic Botany 17, 63–72.
Molecular systematics of the Acmena alliance (Myrtaceae): phylogenetic analyses and evolutionary implications with reference to Australian taxa.CrossRef | 1:CAS:528:DC%2BD2cXitFCgt74%3D&md5=5415f7b403d01685d7110b7e5c90ab74CAS |

Harris W (1987a) Kunzea. In ‘Name changes in the indigenous New Zealand flora, 1960–1986 and nomina nova IV, 1983–1986’. (Eds HE Connor, E Edgar) pp. 134. New Zealand Journal of Botany 25, 115–170.

Harris W (1987b) Knowing kanuka as Kunzea. Canterbury Botanical Society Journal 21, 11–20.

Harris W (1993) ×Kunzspermum hirakimata ‘Karo Hobson Choice’ – a new intergeneric hybrid tea tree cultivar. Horticulture in New Zealand 4, 10–12.

Harris W (1996) Genecological aspects of flowering patterns of populations of Kunzea ericoides and K. sinclairii (Myrtaceae). New Zealand Journal of Botany 34, 333–354.

Harris W, Porter NG, Dawson MI (1992) Observations on biosystematic relationships of Kunzea sinclairii and on an intergeneric hybrid Kunzea sinclairii × Leptospermum scoparium. New Zealand Journal of Botany 30, 213–230.

Jeanes JA (1996). Kunzea. In ‘Flora of Victoria. Vol. 3: Dicotyledons – Winteraceae to Myrtaceae’. (Eds NG Walsh, TJ Entwisle) pp. 1019–1022. (Inkata Press: Melbourne)

Johnson LAS, Briggs BG (1984) Myrtales and Myrtaceae – a phylogenetic analysis. Annals of the Missouri Botanical Garden 71, 700–756.
Myrtales and Myrtaceae – a phylogenetic analysis.CrossRef |

Lee J, Baldwin BG, Gottlieb LD (2002) Phylogeny of Stephanomeria and related genera (Compositae–Lactuceae) based on analysis of 18S–26S nuclear rDNA ITS and ETS sequences. American Journal of Botany 89, 160–168.
Phylogeny of Stephanomeria and related genera (Compositae–Lactuceae) based on analysis of 18S–26S nuclear rDNA ITS and ETS sequences.CrossRef | 1:CAS:528:DC%2BD3sXjvFOksQ%3D%3D&md5=51f0d46e73ac2cd7ede6e6e0b252be8cCAS |

Linder CR, Goertzen LR, Heuvel BV, Franciso-Ortega J, Jansen RK (2000) The complete external transcribed spacer of 18S–26S rDNA: amplification and phylogenetic utility at low taxonomic levels in the Asteraceae and closely allied families. Molecular Phylogenetics and Evolution 14, 285–303.
The complete external transcribed spacer of 18S–26S rDNA: amplification and phylogenetic utility at low taxonomic levels in the Asteraceae and closely allied families.CrossRef | 1:CAS:528:DC%2BD3cXhtFGjs78%3D&md5=8273dc6fcb89a1991af21141162e932fCAS | 10679161PubMed |

Markos S, Baldwin BG (2001) Higher-level relationships and major lineages of Lessingia (Compositae, Astereae) based on nuclear rDNA internal and external transcribed spacer (ITS and ETS) sequences. Systematic Botany 26, 168–183.

O’Brien MM, Quinn CJ, Wilson PG (2000) Molecular systematics of the Leptospermum suballiance (Myrtaceae). Australian Journal of Botany 48, 621–628.
Molecular systematics of the Leptospermum suballiance (Myrtaceae).CrossRef | 1:CAS:528:DC%2BD3cXosFKnt7g%3D&md5=3f7c27e86679130b8de8feaf11d53783CAS |

Perry NB, Van Klink JW, Brennan NJ, Harris W, Anderson RE, Douglas MH, Smallfield BM (1997) Essential oils from New Zealand manuka and kanuka: chemotaxonomy of Kunzea. Phytochemistry 45, 1605–1612.
Essential oils from New Zealand manuka and kanuka: chemotaxonomy of Kunzea.CrossRef | 1:CAS:528:DyaK2sXkvVWktrs%3D&md5=5998caa525eba266926a4ec69b513ba8CAS |

Petterson J (2006) In preparation for a revised flora of New Zealand Vol. 1 – four puzzles in New Zealand Botany that need attention. New Zealand Botanical Society Newsletter 84, 14–15.

Ronquist F, Huelsenbeck JP, van der Mark P (2005) ‘MrBayes 3.1. Draft Manual.’ Available at http://mrbayes.csit.fsu.edu/wiki/index.php/Manual [accessed July 2010].

Singer RJ, Burgman MA (1999) The regeneration ecology of Kunzea ericoides (A.Rich.) J.Thompson at Coranderrk Reserve, Healesville. Australian Journal of Ecology 24, 18–24.
The regeneration ecology of Kunzea ericoides (A.Rich.) J.Thompson at Coranderrk Reserve, Healesville.CrossRef |

Steane DA, Nicolle D, McKinnon GE, Vaillancourt RE, Potts BM (2002) Higher-level relationships among the eucalypts are resolved by ITS-sequence data. Australian Systematic Botany 15, 49–62.
Higher-level relationships among the eucalypts are resolved by ITS-sequence data.CrossRef |

Swofford DL (2002) ‘PAUP*. Phylogenetic analysis using parsimony (and other methods), version 4.0b10.’ (Sinauer Associates: Sunderland, MA)

Thompson J (1983) Redefinitions and nomenclatural changes within the Leptospermum suballiance of Myrtaceae. Telopea 2, 379–383.

Thompson J (1989) A revision of the genus Leptospermum (Myrtaceae). Telopea 3, 301–446.

Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research 25, 4876–4882.
The ClustalX windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools.CrossRef | 1:CAS:528:DyaK1cXntFyntQ%3D%3D&md5=8896c6413165523e7c66eaaad4496c2eCAS | 9396791PubMed |

Tierney DA, Wardle GM (2005) Differential seed ecology in the shrubs Kunzea rupestris, K. capitata and associated hybrids (Myrtaceae): the function of thin walled fruit in fire prone vegetation. Australian Journal of Botany 53, 313–321.
Differential seed ecology in the shrubs Kunzea rupestris, K. capitata and associated hybrids (Myrtaceae): the function of thin walled fruit in fire prone vegetation.CrossRef |

Toelken HR (1981a) Proposal to conserve Kunzea Reichb. over Kunzia Spreng. Taxon 30, 350
Proposal to conserve Kunzea Reichb. over Kunzia Spreng.CrossRef |

Toelken HR (1981b) Proposal to conserve Kunzea Reichenbach over Tillospermum Salisbury (Myrtaceae). Taxon 30, 828
Proposal to conserve Kunzea Reichenbach over Tillospermum Salisbury (Myrtaceae).CrossRef |

Toelken HR (1996) A revision of the genus Kunzea (Myrtaceae) I. The Western Australia section Zeanuk. Journal of the Adelaide Botanic Gardens 17, 29–106.

Toelken HR, Craig GF (2007) Kunzea acicularis, K. strigosa and K. similis subsp. mediterranea (Myrtaceae) – new taxa from near Ravensthorpe, Western Australia. Nutysia 17, 392–393.

Trudgen ME, Keighery GJ (1983) Angasomyrtus, a new genus of Myrtaceae (Leptosperminae) from Western Australia. Nuytsia 4, 435–439.

White T, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for photosynthesis. In ‘PCR protocols: a guide to methods and applications’. (Eds M. Innis, D. Gelfand, J. Sninsky, T. White) pp. 315–322. (Academic Press: San Diego, CA)

Wichman SR (2000) Molecular phylogeny of Coprosma based on rDNA ITS and ETS sequences. MSc Thesis, University of Auckland.

Wichman SR, Wright SD, Cameron EK, Gardner RC (2002) Elevated genetic heterogeneity and Pleistocene climatic instability: inferences from nrDNA in Coprosma (Rubiaceae). Journal of Biogeography 29, 943–954.
Elevated genetic heterogeneity and Pleistocene climatic instability: inferences from nrDNA in Coprosma (Rubiaceae).CrossRef |

Wilson PG (1991). Kunzea. In ‘Flora of New South Wales, Vol. 2’. (Ed. GJ Harden) pp. 150–154. (New South Wales University Press: Sydney)

Wilson PG, O’Brien MM, Heslewood MM, Quinn CJ (2005) Relationships within Myrtaceae sensu lato based on matK phylogeny. Plant Systematics and Evolution 251, 3–19.
Relationships within Myrtaceae sensu lato based on matK phylogeny.CrossRef |

Wright O (1950) ‘New Zealand 1826–1827; an English translation of the voyage de l’Astrolabe in New Zealand waters, with an introductory essay by Olive Wright.’ (Wingfield Press: Wellington, New Zealand)

Wright SD, Yong CG, Dawson JW, Whittaker DJ, Gardner RC (2000) Riding the ice age El Nino? Pacific biogeography and evolution of Metrosideros subg. Metrosideros (Myrtaceae) inferred from nuclear ribosomal DNA. Proceedings of the Natural Academy of Sciences, USA 97, 4118–4123.
Riding the ice age El Nino? Pacific biogeography and evolution of Metrosideros subg. Metrosideros (Myrtaceae) inferred from nuclear ribosomal DNA.CrossRef | 1:CAS:528:DC%2BD3cXislSgtbg%3D&md5=0dd37be26cd9d6815f4d5a5be0154fe9CAS |

Wright SD, Yong CG, Wichman SR, Dawson JW, Gardner RC (2001) Stepping stones to Hawaii: a trans-equatorial dispersal pathway for Metrosideros (Myrtaceae) inferred from nrDNA (ITS + ETS). Journal of Biogeography 28, 769–774.
Stepping stones to Hawaii: a trans-equatorial dispersal pathway for Metrosideros (Myrtaceae) inferred from nrDNA (ITS + ETS).CrossRef |

Wrigley JW, Fagg M (1993) ‘Bottlebrushes paperbarks and tea trees – and all other plants in the Leptospermum alliance.’ (Angus & Robertson: Sydney)



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