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RESEARCH ARTICLE

Carbon and nitrogen in particle-size fractions of organic matter of soils fertilised with surface and injected applications of pig slurry

Cremildo António Luís Francisco A , Arcângelo Loss https://orcid.org/0000-0002-3005-6158 B * , Gustavo Brunetto C , Rogério Gonzatto C , Sandro José Giacomini C , Celso Aita C , Marisa de Cássia Piccolo D , José Luiz Rodrigues Torres https://orcid.org/0000-0003-4211-4340 E , Carina Marchezan C , Gustavo Scopel C and Ricardo Fagan Vidal C
+ Author Affiliations
- Author Affiliations

A Agriculture Division, Polytechnic Higher Education Institute of Manica, Mozambique.

B Federal University of Santa Catarina, Florianópolis, Santa Catarina, Brazil.

C Federal University of Santa Maria, Santa Maria, Rio Grande do Sul, Brazil.

D Center of Nuclear Energy in Agriculture (CENA), University of São Paulo, Piracicaba, São Paulo, Brazil.

E Instituto Federal do Triângulo Mineiro, Uberaba, Minas Gerais, Brazil.

* Correspondence to: arcangelo.loss@ufsc.br

Handling Editor: Iris Vogeler

Soil Research 60(1) 65-72 https://doi.org/10.1071/SR21020
Submitted: 20 January 2021  Accepted: 6 July 2021   Published: 13 October 2021

© 2022 The Author(s) (or their employer(s)). Published by CSIRO Publishing

Abstract

The injection of pig slurry in the soil, associated with the no-tillage system and the reduction of carbon and nitrogen losses through volatilisation, can positively influence the carbon and nitrogen contents in the soil organic matter (SOM) fractions. The objective of the present study was to evaluate the effect of surface and injected applications of pig slurry to soils under a no-tillage system on soil organic carbon (SOC) and soil total nitrogen (STN) contents in particle-size fractions of the SOM. The no-tillage system included a crop rotation with grass species, summer maize, and winter black oat and wheat grown from 2011 to 2015. The treatments consisted of injected application of pig slurry, surface applications of pig slurry, application of chemical fertiliser (NPK), and a control with no soil fertiliser application. The 0–5, 5–10, and 10–20 cm soil layers were evaluated for SOC and STN contents, and for particle-size fractions: particulate organic carbon, particulate organic nitrogen, mineral-associated organic carbon, and mineral-associated organic nitrogen. We also evaluated the carbon and nitrogen contents and mass in the light organic matter. The injection of pig slurry increased carbon and nitrogen contents of all SOM fractions compared to surface application of pig slurry. These results suggest that the use of injected pig slurry to the soil under crop rotation in no-tillage reduces carbon and nitrogen losses by volatilisation and emissions to the atmosphere. We recommend the application of pig slurry by injecting in soil because it promotes higher increases in the SOM fractions. This form of application of pig slurry to the soil was shown as an effective and environmentally sustainable method for the conservation and improvement of soil quality.

Keywords: injected application, light fraction, mineral-associated organic carbon, mineral-associated organic nitrogen, no-tillage system, particulate organic matter, particulate organic nitrogen, swine manure.


References

Aita C, Chantigny MH, Gonzatto R, Miola ECC, Rochette P, Pujol SB, Santos DB, Giacomini DA, Giacomini SJ (2019) Winter-season gaseous nitrogen emissions in subtropical climate: impacts of pig slurry injection and nitrification inhibitor. Journal of Environmental Quality 48, 1414–1426.
Winter-season gaseous nitrogen emissions in subtropical climate: impacts of pig slurry injection and nitrification inhibitor.Crossref | GoogleScholarGoogle Scholar | 31589701PubMed |

Anderson JM, Ingram JSI (1994) Tropical soil biology and fertility: a handbook of methods. Soil Science 157, 265
Tropical soil biology and fertility: a handbook of methods.Crossref | GoogleScholarGoogle Scholar |

Brancalião SR, Moraes MH (2008) Alterações de alguns atributos físicos e das frações húmicas de um Nitossolo vermelho na sucessão milheto-soja em sistema plantio direto. Revista Brasileira de Ciência do Solo 32, 393–404.
Alterações de alguns atributos físicos e das frações húmicas de um Nitossolo vermelho na sucessão milheto-soja em sistema plantio direto.Crossref | GoogleScholarGoogle Scholar |

Cambardella CA, Elliott ET (1994) Carbon and Nitrogen dynamics of soil organic matter fractions from cultivated grassland soils. Soil Science Society of America Journal 58, 123–130.
Carbon and Nitrogen dynamics of soil organic matter fractions from cultivated grassland soils.Crossref | GoogleScholarGoogle Scholar |

Chen J, Xiao W, Zheng C, Zhu B (2020) Nitrogen addition has contrasting effects on particulate and mineral-associated soil organic carbon in a subtropical forest. Soil Biology and Biochemistry 142, e107708
Nitrogen addition has contrasting effects on particulate and mineral-associated soil organic carbon in a subtropical forest.Crossref | GoogleScholarGoogle Scholar |

Comissão de Química e Fertilidade do Solo – CQFS-RS/SC (2004) ‘Manual de adubação e calagem para os estados do Rio Grande do Sul e Santa Catarina’. 10th edn. (Sociedade Brasileira de Ciência do Solo: Porto Alegre, Brazil)

Conceição PC, Dieckow J, Bayer C (2013) Combined role of no tillage and cropping systems in soil carbon stocks and stabilization. Soil and Tillage Research 129, 40–47.
Combined role of no tillage and cropping systems in soil carbon stocks and stabilization.Crossref | GoogleScholarGoogle Scholar |

Cotrufo MF, Wallenstein MD, Boot CM, Denef K, Paul E (2013) The Microbial Efficiency-Matrix Stabilization (MEMS) framework integrates plant litter decomposition with soil organic matter stabilization: do labile plant inputs form stable soil organic matter? Global Change Biology 19, 988–995.
The Microbial Efficiency-Matrix Stabilization (MEMS) framework integrates plant litter decomposition with soil organic matter stabilization: do labile plant inputs form stable soil organic matter?Crossref | GoogleScholarGoogle Scholar | 23504877PubMed |

de Oliveira GF, Roters DF, Prazeres MS (2017) Aplicação de diferentes formas de fertilizante orgânico no solo para o rendimento da cultura do Zea mays. Revista Agroecossistemas 9, 11–20.
Aplicação de diferentes formas de fertilizante orgânico no solo para o rendimento da cultura do Zea mays.Crossref | GoogleScholarGoogle Scholar |

Dortzbach D, Araujo IS, Pandolfo C, Veiga M (2013) Carbono e nitrogênio no solo e na biomassa microbiana em glebas com diferentes usos e períodos de aplicação de dejetos líquidos de suínos. Agropec Catarinense 26, 69–73.

dos Santos LH, Loss A, Canton L, Souza M, Ferreira GW, Kurtz C, Comin JJ (2018) Carbon and nitrogen content in granulometric fractions of organic matter in soil aggregates under no-tillage and conventional tillage planting systems for onions. Idesia (Arica) 36, 10–20.
Carbon and nitrogen content in granulometric fractions of organic matter in soil aggregates under no-tillage and conventional tillage planting systems for onions.Crossref | GoogleScholarGoogle Scholar |

Embrapa – Empresa Brasileira de Pesquisa Agropecuária (1997) ‘Manual de métodos de análise de solo’. 2nd edn. (Documentos, 1). (Embrapa-CNPS: Rio de Janeiro, Brazil)

Embrapa – Empresa Brasileira de Pesquisa Agropecuária (2013) ‘Sistema Brasileiro de Classificação de Solos’. 3rd edn. (Embrapa Solos: Brasília, Brazil)

Faccin FC, Marchetti ME, Serra AP, Ensinas SC (2016) Frações granulométricas da matéria orgânica do solo em consórcio de milho safrinha com capim-marandu sob fontes de nitrogênio. Pesquisa Agropecuaria Brasiliera 51, 2000–2009.
Frações granulométricas da matéria orgânica do solo em consórcio de milho safrinha com capim-marandu sob fontes de nitrogênio.Crossref | GoogleScholarGoogle Scholar |

Ferreira G, Benedet L, Trapp T, Lima A, Junior V, Loss A, Lourenzi C, Comin J (2021) Soil aggregation indexes and chemical and physical attributes of aggregates in a Typic Hapludult fertilized with swine manure and mineral fertilizer. International Journal of Recycling Organic Waste in Agriculture 10, 1–17.
Soil aggregation indexes and chemical and physical attributes of aggregates in a Typic Hapludult fertilized with swine manure and mineral fertilizer.Crossref | GoogleScholarGoogle Scholar |

Ferreira LB, Loss A, Giumbelli LD, Ventura BS, Souza M, Mafra ÁL, Kurtz C, Comin JJ, Brunetto G (2018) Organic carbon and nitrogen contents and their fractions in soils with onion crops in different management systems. Soil Research 56, 846–855.
Organic carbon and nitrogen contents and their fractions in soils with onion crops in different management systems.Crossref | GoogleScholarGoogle Scholar |

Fonte SJ, Yeboah E, Ofori P, Quansah GW, Vanlauwe B, Six J (2009) Fertilizer and residue quality effects on organic matter stabilization in soil aggregates. Soil Science Society of America Journal 73, 961–966.
Fertilizer and residue quality effects on organic matter stabilization in soil aggregates.Crossref | GoogleScholarGoogle Scholar |

Freire FM, Vasconcellos CA, de França GE (2001) Manejo da fertilidade do solo em sistema de plantio direto. Informe Agropecuário 22, 49–56.

Giumbelli LD, Loss A, Kurtz C, Mafra ÁL, Piccolo MDC, Torres JLR, Lourenzi CR, Brunetto G, Comin JJ (2021) Combinations of plant species for rotation with onion crops: effects on the light fraction, carbon, and nitrogen contents in granulometric fractions of the soil organic matter. Journal of Agricultural Studies 9, 202–230.
Combinations of plant species for rotation with onion crops: effects on the light fraction, carbon, and nitrogen contents in granulometric fractions of the soil organic matter.Crossref | GoogleScholarGoogle Scholar |

Golchin A, Oades JM, Skjemstad JO, Clarke P (1994) Study of free and occluded particulate organic matter in soils by solid state 13C Cp/MAS NMR spectroscopy and scanning electron microscopy. Australian Journal of Soil Research 32, 285–309.

Gonzatto R, Aita C, Bélanger G, Chantigny MH, Miola ECC, Pujol SB, Dessbesel A, Giacomini SJ (2017) Response of no-till grain crops to pig slurry application methods and a nitrification inhibitor. Agronomy Journal 109, 1687–1696.
Response of no-till grain crops to pig slurry application methods and a nitrification inhibitor.Crossref | GoogleScholarGoogle Scholar |

He YT, Zhang WJ, Xu MG, Tong XG, Sun FX, Wang JZ, Huang SM, Zhu P, He XH (2015) Long-term combined chemical and manure fertilizations increase soil organic carbon and total nitrogen in aggregate fractions at three typical cropland soils in China. Science of the Total Environment 532, 635–644.
Long-term combined chemical and manure fertilizations increase soil organic carbon and total nitrogen in aggregate fractions at three typical cropland soils in China.Crossref | GoogleScholarGoogle Scholar |

Lavallee JM, Soong JL, Cotrufo MF (2020) Conceptualizing soil organic matter into particulate and mineral-associated forms to address global change in the 21st century. Global Change Biology 26, 261–273.
Conceptualizing soil organic matter into particulate and mineral-associated forms to address global change in the 21st century.Crossref | GoogleScholarGoogle Scholar | 31587451PubMed |

Lima CEP, Fontenelle MR, Madeira NR, da Silva J, Guedes ÍMR, Silva LRB, Soares DC (2016) Compartimentos de carbono orgânico em Latossolo cultivado com hortaliças sob diferentes manejos. Pesquisa Agropecuária Brasileira 51, 378–387.
Compartimentos de carbono orgânico em Latossolo cultivado com hortaliças sob diferentes manejos.Crossref | GoogleScholarGoogle Scholar |

Loss A, Costa EM, Pereira MG, Beutler SJ (2014) Agregação, matéria orgânica leve e carbono mineralizável em agregados do solo. Revista de la Facultad de Agronomía, La Plata 113, 1–8.

Loss A, Couto RR, Brunetto G, Veiga M, Toselli M, Baldi E (2019) Animal manure as fertilizer: changes in soil attributes, productivity and food composition. International Journal of Research – Granthaalayah 7, 307–331.
Animal manure as fertilizer: changes in soil attributes, productivity and food composition.Crossref | GoogleScholarGoogle Scholar |

Loss A, Lima Moraes AG, Pereira MG, Silva EMR, Anjos LHC (2010) Carbono, matéria orgânica leve e frações oxidáveis do carbono orgânico sob diferentes sistemas de produção orgânica. Comunicata Scientiae 1, 57–67.

Loss A, Lourenzi CR, das Santos Junior E, Mergen Junior CA, Benedet L, Pereira MG, Piccolo MDC, Brunetto G, Lovato PE, Comin JJ (2017) Carbon, Nitrogen and natural abundance of 13C and 15N in biogenic and physicogenic aggregates in a soil with 10 years of pig manure application. Soil and Tillage Research 166, 52–58.
Carbon, Nitrogen and natural abundance of 13C and 15N in biogenic and physicogenic aggregates in a soil with 10 years of pig manure application.Crossref | GoogleScholarGoogle Scholar |

Malobane ME, Nciizah AD, Mudau FN, Wakindiki IIC (2020) Tillage, crop rotation and crop residue management effects on nutrient availability in a sweet sorghum-based cropping system in marginal soils of South Africa. Agronomy 10, 776
Tillage, crop rotation and crop residue management effects on nutrient availability in a sweet sorghum-based cropping system in marginal soils of South Africa.Crossref | GoogleScholarGoogle Scholar |

Mellek JE, Dieckow J, da Silva VL, Favaretto N, Pauletti V, Vezzani FM, de Souza JLM (2010) Dairy liquid manure and no-tillage: physical and hydraulic properties and carbon stocks in a Cambisol of Southern Brazil. Soil and Tillage Research 110, 69–76.
Dairy liquid manure and no-tillage: physical and hydraulic properties and carbon stocks in a Cambisol of Southern Brazil.Crossref | GoogleScholarGoogle Scholar |

Poeplau C, Don A, Six J, Kaiser M, Benbi D, Chenu C, Nieder R (2018) Isolating organic carbon fractions with varying turnover rates in temperate agricultural soils – a comprehensive method comparison. Soil Biology and Biochemistry 125, 10–26.
Isolating organic carbon fractions with varying turnover rates in temperate agricultural soils – a comprehensive method comparison.Crossref | GoogleScholarGoogle Scholar |

Seidel EP, Gonçalves Junior AC, Vanin JP, Strey L, Schwantes D, Nacke H (2010) Aplicação de dejetos de suínos na cultura do milho cultivado em sistema de plantio direto. Acta Scientiarum Technology 32, 113–117.

Silveira FM, Favaretto N, Dieckow J, Pauletti V, Vezzani FM, Silva EDB (2011) Dejeto líquido bovino em plantio direto: perdas de carbono e nitrogénio por escoamento superficial. Revista Brasileira de Ciência do Solo 35, 1759–1767.

Smith KA, Jackson DR, Pepper TJ (2001) Nutrient losses by surface run-off following the application of organic manures to arable land. 1. Nitrogen. Environmental Pollution 112, 41–51.
Nutrient losses by surface run-off following the application of organic manures to arable land. 1. Nitrogen.Crossref | GoogleScholarGoogle Scholar | 11202653PubMed |

Soil Survey Staff (2014) ‘Keys to soil taxonomy’. 12th edn. (USDA-Natural Resources Conservation Service: Washington, DC, USA)

Tedesco MJ, Gianello C, Bissani CA, Bohnen H, Volkweiss SJ (1995) ‘Analise de solo, plantas e outros materiais’. 2nd edn. (Departamento de solos: Porto Alegre, Brazil)

Ventura BS, Loss A, Comin JJ, Sepulveda CM, Lovato PE, Brunetto G (2020) Carbono, nitrogênio e frações granulométricas em agregados biogênicos e fisiogênicos de um solo com histórico de 10 anos de aplicações sucessivas de dejetos suínos. Research Society and Development 9, e5139108776
Carbono, nitrogênio e frações granulométricas em agregados biogênicos e fisiogênicos de um solo com histórico de 10 anos de aplicações sucessivas de dejetos suínos.Crossref | GoogleScholarGoogle Scholar |

Wiesmeier M, Hübner R, Spörlein P, Geuß U, Hangen E, Reischl A, Schilling B, von Lützow M, Kögel-Knabner I (2014) Carbon sequestration potential of soils in southeast Germany derived from stable soil organic carbon saturation. Global Change Biology 20, 653–665.
Carbon sequestration potential of soils in southeast Germany derived from stable soil organic carbon saturation.Crossref | GoogleScholarGoogle Scholar | 24038905PubMed |

World Reference Base for Soil Resources (2015) International soil classification system for naming soils and creating legends for soil maps. World Soil Resources Reports No. 106. FAO, Rome, Italy.

Yagüe MR, Bosch-Serra AD, Antúnez M, Boixadera J (2012) Pig slurry and mineral fertilization strategies effects on soil quality: macroaggregate stability and organic matter fractions. Science of The Total Environment 438, 18–224.
Pig slurry and mineral fertilization strategies effects on soil quality: macroaggregate stability and organic matter fractions.Crossref | GoogleScholarGoogle Scholar |

Zaman M, Saggar S, Blennerhassett JD, Singh J (2009) Effect of urease and nitrification inhibitors on N transformation, gaseous emissions of ammonia and nitrous oxide, pasture yield and N uptake in grazed pasture system. Soil Biology and Biochemistry 41, 1270–1280.
Effect of urease and nitrification inhibitors on N transformation, gaseous emissions of ammonia and nitrous oxide, pasture yield and N uptake in grazed pasture system.Crossref | GoogleScholarGoogle Scholar |