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Article << Previous     |     Next >>   Contents Vol 25(7)

Effects of oocyte quality, incubation time and maturation environment on the number of chromosomal abnormalities in IVF-derived early bovine embryos

Sebastian Demyda-Peyrás A D , Jesus Dorado B , Manuel Hidalgo B , Jaouad Anter A , Leonardo De Luca C , Enrique Genero C and Miguel Moreno-Millán A

A Department of Genetics, University of Cordoba, CN IV KM 396, Campus Rabanales CP 14071, Gregor Mendel Building, Cordoba, Spain.
B Animal Reproduction Group, Department of Medicine and Animal Surgery, Faculty of Veterinary Medicine, University of Cordoba, CN IV KM 396, Campus Rabanales CP 14071, Spain.
C Animal Production Department, Lomas de Zamora University, Ruta 4 Km 2 CP 1834, Llavallol, Bs As, Argentina.
D Corresponding author. Email: ge2depes@uco.es

Reproduction, Fertility and Development 25(7) 1077-1084 http://dx.doi.org/10.1071/RD12140
Submitted: 1 May 2012  Accepted: 27 September 2012   Published: 27 November 2012


 
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Abstract

Chromosomal aberrations are one of the major causes of embryo developmental failures in mammals. The occurrence of these types of abnormalities is higher in in vitro-produced (IVP) embryos. The aim of the present study was to investigate the effect of oocyte morphology and maturation conditions on the rate of chromosomal abnormalities in bovine preimplantational embryos. To this end, 790 early cattle embryos derived from oocytes with different morphologies and matured under different conditions, including maturation period (24 v. 36 h) and maturation media (five different serum supplements in TCM-199), were evaluated cytogenetically in three sequential experiments. The rates of normal diploidy and abnormal haploidy, polyploidy and aneuploidy were determined in each embryo. Throughout all the experiments, the rate of chromosomal abnormalities was significantly (P < 0.05) affected by oocyte morphology and maturation conditions (maturation time and culture medium). Lower morphological quality was associated with a high rate of chromosome abnormalities (P < 0.05). Moreover, polyploidy was associated with increased maturation time (P < 0.01), whereas the maturation medium significantly (P < 0.05) affected the rates of haploidy and polyploidy. In general, supplementing the maturation medium with oestrous cow serum or fetal calf serum resulted in higher rates of chromosomal aberrations (P < 0.05) compared with the other serum supplements tested (bovine steer serum, anoestroues cow serum, bovine amniotic fluid and bovine serum albumin). On the basis of the results of the present study, we conclude that the morphological quality of oocytes and the maturation conditions affect the rate of chromosomal abnormalities in IVP bovine embryos.

Additional keywords: aneuploidy, cattle, chromosome analysis, developmental failures, in vitro produced (IVP) embryos, maturation time, polyploidy, serum supplementation.


References

A’arabi, S. Y., Roussel, J. D., and Chandler, J. E. (1997). Chromosomal analysis of mammalian oocytes matured in vitro with various culture systems. Theriogenology 48, 1173–1183.
CrossRef | CAS | PubMed |

Alexander, B., Coppola, G., Berardina, D., Rho, G.J., St John, E., Bett, D. H., and King, W. A. (2006). The effect of 6-dimethylaminopurine (6-DMAP) and cycloheximide (CHX) on the development and chromosomal complement of sheep parthenogenetic and nuclear transfer embryos. Mol. Reprod. Dev. 73, 20–30.
CrossRef | CAS | PubMed |

Barrett, S. L., and Albertini, D. F. (2010). Cumulus cell contact during oocyte maturation in mice regulates meiotic spindle positioning and enhances developmental competence. J. Assist. Reprod. Genet. 27, 29–39.
CrossRef | PubMed |

Bilodeau-Goeseels, S. (2006). Effects of culture media and energy sources on the inhibition of nuclear maturation in bovine oocytes. Theriogenology 66, 297–306.
CrossRef | PubMed |

Blondin, P., and Sirard, M. A. (1995). Oocyte and follicular morphology as determining characteristics for developmental competence in bovine oocytes. Mol. Reprod. Dev. 41, 54–62.
CrossRef | CAS | PubMed |

Cetica, P. D., Dalvit, G. C., and Beconi, M. T. (1999). Study of evaluation criteria used for in vitro bovine oocyte selection and maturation. Biocell 23, 125–133.
| CAS | PubMed |

Christopikou, D., Karamalegos, C., Doriza, S., Argyrou, M., Sisi, P., Davies, S., and Mastrominas, M. (2010). Spindle and chromosome configurations of human oocytes matured in vitro in two different culture media. Reprod. Biomed. Online 20, 639–648.
CrossRef | CAS | PubMed |

Coticchio, G., Sereni, E., Serrao, L., Mazzone, S., Iadarola, I., and Borini, A. (2004). What criteria for the definition of oocyte quality? Ann. N. Y. Acad. Sci. 1034, 132–144.
CrossRef | PubMed |

Dale, B., and Defelice, L. (2011). Polyspermy prevention: facts and artifacts? J. Assist. Reprod. Genet. 28, 199–207.
CrossRef | PubMed |

Dieleman, S. J., Hendriksen, P. J. M., Viuff, D., Thomsen, P. D., Hyttel, P., Knijn, H. M., Wrenzycki, C., Kruip, T. A. M., Niemann, H., Gadella, B. M., Bevers, M. M., and Vos, P. L. A. M. (2002). Effects of in vivo prematuration and in vivo final maturation on developmental capacity and quality of pre-implantation embryos. Theriogenology 57, 5–20.
CrossRef | CAS | PubMed |

Ebner, T., Moser, M., Sommergruber, M., and Tews, G. (2003). Selection based on morphological assessment of oocytes and embryos at different stages of preimplantation development: a review. Hum. Reprod. Update 9, 251–262.
CrossRef | CAS | PubMed |

Eichenlaub-Ritter, U., Wieczorek, M., Lüke, S., and Seidel, T. (2011). Age related changes in mitochondrial function and new approaches to study redox regulation in mammalian oocytes in response to age or maturation conditions. Mitochondrion 11, 783–796.
CrossRef | CAS | PubMed |

Frumkin, T., Malcov, M., Yaron, Y., and Ben-Yosef, D. (2008). Elucidating the origin of chromosomal aberrations in IVF embryos by preimplantation genetic analysis. Mol. Cell. Endocrinol. 282, 112–119.
CrossRef | CAS | PubMed |

Fukui, Y., and Ono, H. (1989). Effects of sera, hormones and granulosa cells added to culture medium for in-vitro maturation, fertilization, cleavage and development of bovine oocytes. J. Reprod. Fertil. 86, 501–506.
CrossRef | CAS | PubMed |

Funahashi, H. (2003). Polyspermic penetration in porcine IVM–IVF systems. Reprod. Fertil. Dev. 15, 167–177.
CrossRef | PubMed |

Garcia-Herreros, M., Carter, T. F., Villagmez, D. A. F., MacAulay, A. D., Rath, D., King, W. A., and Lonergan, P. (2010). Incidence of chromosomal abnormalities in bovine blastocysts derived from unsorted and sex-sorted spermatozoa. Reprod. Fertil. Dev. 22, 1272–1278.
CrossRef | CAS | PubMed |

Gliedt, D. W., Rosenkrans, C. F., Rorie, R. W., and Rakes, J. M. (1996). Effects of oocyte maturation length, sperm capacitation time, and heparin on bovine embryo development. J. Dairy Sci. 79, 532–535.
CrossRef | CAS | PubMed |

Hawk, H. W., and Wall, R. J. (1994). Improved yields of bovine blastocysts from in vitro-produced oocytes. II. Media and co-culture cells. Theriogenology 41, 1585–1594.
CrossRef |

Jakobsen, A. S., Thomsen, P. D., and Avery, B. (2006). Few polyploid blastomeres in morphologically superior bovine embryos produced in vitro. Theriogenology 65, 870–881.
CrossRef | PubMed |

Kawarsky, S. J., Basrur, P. K., Stubbings, R. B., Hansen, P. J., and King, W. A. (1996). Chromosomal abnormalities in bovine embryos and their influence on development. Biol. Reprod. 54, 53–59.
CrossRef | CAS | PubMed |

King, W. A. (1990). Chromosome abnormalities and pregnancy failure in domestic animals. Adv. Vet. Sci. Comp. Med. 34, 229–250.

King, W. A., Coppola, G., Alexander, B., Mastromonaco, G., Perrault, S., Nino-Soto, M. I., Pinton, A., Joudrey, E. M., and Betts, D. H. (2006). The impact of chromosomal alteration on embryo development. Theriogenology 65, 166–177.
CrossRef | CAS | PubMed |

Krisher, R. L. (2004). The effect of oocyte quality on development. J. Anim. Sci. 82, E14–E23.
| PubMed |

Lechniak, D., Świtoński, M., and Sosnowski, M. (1996). The incidence of bovine diploid oocytes matured in vitro. Theriogenology 46, 267–277.
CrossRef | CAS | PubMed |

Lechniak, D., Szczepankiewicz, D., Kauss, D., Szulc, J., and Szydłowski, M. (2005). IVM media, oocyte diameter and donor genotype at RYR1 locus in relation to the incidence of porcine diploid oocytes after maturation in vitro. Theriogenology 64, 202–212.
CrossRef | CAS | PubMed |

Liu, L., and Keefe, D. L. (2002). Ageing-associated aberration in meiosis of occytes from senescence-accelerated mice. Hum. Reprod. 17, 2678–2685.
CrossRef | CAS | PubMed |

Liu, S., Feng, H. L., Marchesi, D., Chen, Z. J., and Hershlag, A. (2011). Dose-dependent effects of gonadotropin on oocyte developmental competence and apoptosis. Reprod. Fertil. Dev. 23, 990–996.
CrossRef | CAS | PubMed |

Lonergan, P. (2007). State-of-the-art embryo technologies in cattle. Soc. Reprod. Fertil. Suppl. 64, 315–325.
| CAS | PubMed |

Lonergan, P., Pedersen, H. G., Rizos, D., Greve, T., Thomsen, P. D., Fair, T., Evans, A., and Boland, M. P. (2004). Effect of the post-fertilization culture environment on the incidence of chromosome aberrations in bovine blastocysts. Biol. Reprod. 71, 1096–1100.
CrossRef | CAS | PubMed |

Long, C. R., Damiani, P., Pinto-Correia, C., MacLean, R. A., Duby, R. T., and Robl, J. M. (1994). Morphology and subsequent development in culture of bovine oocytes matured in vitro under various conditions of fertilization. J. Reprod. Fertil. 102, 361–369.
CrossRef | CAS | PubMed |

Máximo, D. M., Martins da Silva, I. G., Mondadori, R. G., Neves, J. P., and Lucci, C. M. (2012). Ultrastructural characteristics of sheep oocytes during in vitro maturation (IVM). Small Rumin. Res. 105, 210–215.
CrossRef |

Mermillod, P., Schmaltz, B., Perreau, C., Tsikis, G., Martinot, E., Cordova, A., and Locatelli, Y. (2010). Early development of ruminant embryos, autonomous process or the result of a positive dialog with surrounding maternal tissues? Acta Sci. Vet. 38, S521–S533.

Nagano, M., Katagiri, S., and Takahashi, Y. (2006). Relationship between bovine oocyte morphology and in vitro developmental potential. Zygote 14, 53–61.
CrossRef | CAS | PubMed |

Nicodemo, D., Pauciullo, A., Cosenza, G., Peretti, V., Perucatti, A., Di Meo, G. P., Ramunno, L., Iannuzzi, L., Rubes, J., and Di Berardino, D. (2010). Frequency of aneuploidy in in vitro-matured MII oocytes and corresponding first polar bodies in two dairy cattle (Bos taurus) breeds as determined by dual-color fluorescent in situ hybridization. Theriogenology 73, 523–529.
CrossRef | CAS | PubMed |

Niwa, K. (1993). Effectiveness of in vitro maturation and in vitro fertilization techniques in pigs. J. Reprod. Fertil. Suppl. 48, 49–59.
| CAS | PubMed |

Ocaña-Quero, J. M., Pinedo-Merlin, M., and Moreno-Millan, M. (1999a). Cytogenetic study of in vitro-derived bovine embryos. Vet. J. 158, 228–233.
CrossRef | PubMed |

Ocaña-Quero, J. M., Pinedo-Merlin, M., and Moreno-Millan, M. (1999b). Influence of follicle size, medium, temperature and time on the incidence of diploid bovine oocytes matured in vitro. Theriogenology 51, 667–672.
CrossRef | PubMed |

Ott, M., Schernthaner, W., Sinowatz, F., and Wolf, E. (2002). Effects of bovine serum albumin and estrous cow serum on development and ultrastructure of in vitro-produced porcine embryos. Anat. Histol. Embryol. 31, 151–157.
CrossRef | PubMed |

Pan, H., Ma, P., Zhu, W., and Schultz, R. M. (2008). Age-associated increase in aneuploidy and changes in gene expression in mouse eggs. Dev. Biol. 316, 397–407.
CrossRef | CAS | PubMed |

Parrish, J. J., Susko-Parrish, J. L., Critser, E. S., Eyestone, W. H., and First, N. L. (1986). Bovine in vitro fertilization with frozen-thawed semen. Theriogenology 25, 591–600.
CrossRef | CAS | PubMed |

Pereira, M. M., MacHado, M. A., Costa, F. Q., Serapiao, R. V., Viana, J. H. M., and Camargo, L. S. A. (2010). Effect of oxygen tension and serum during IVM on developmental competence of bovine oocytes. Reprod. Fertil. Dev. 22, 1074–1082.
CrossRef | PubMed |

Pontes, J. H. F., Nonato-Junior, I., Sanches, B. V., Ereno-Junior, J. C., Uvo, S., Barreiros, T. R. R., Oliveira, J. A., Hasler, J. F., and Seneda, M. M. (2009). Comparison of embryo yield and pregnancy rate between in vivo and in vitro methods in the same Nelore (Bos indicus) donor cows. Theriogenology 71, 690–697.
CrossRef | CAS |

Pontes, J. H. F., Silva, K. C. F., Basso, A. C., Rigo, A. G., Ferreira, C. R., Santos, G. M. G., Sanches, B. V., Porcionato, J. P. F., Vieira, P. H. S., Faifer, F. S., Sterza, F. A. M., Schenk, J. L., and Seneda, M. M. (2010). Large-scale in vitro embryo production and pregnancy rates from Bos taurus, Bos indicus, and indicus–taurus dairy cows using sexed sperm. Theriogenology 74, 1349–1355.
CrossRef | CAS |

Purpera, M. N., Giraldo, A. M., Ballard, C. B., Hylan, D., Godke, R. A., and Bondioli, K. R. (2009). Effects of culture medium and protein supplementation on mRNA expression of in vitro produced bovine embryos. Mol. Reprod. Dev. 76, 783–793.
CrossRef | CAS | PubMed |

Rambags, B. P. B., Krijtenburg, P. J., Van Drie, H. F., Lazzari, G., Galli, C., Pearson, P. L., Colenbrander, B., and Stout, T. A. E. (2005). Numerical chromosomal abnormalities in equine embryos produced in vivo and in vitro. Mol. Reprod. Dev. 72, 77–87.
CrossRef | CAS |

Räty, M., Ketoja, E., Pitkänen, T., Ahola, V., Kananen, K., and Peippo, J. (2011). In vitro maturation supplements affect developmental competence of bovine cumulus–oocyte complexes and embryo quality after vitrification. Cryobiology 63, 245–255.
CrossRef | PubMed |

Rizos, D., Ward, F., Duffy, P., Boland, M. P., and Lonergan, P. (2002). Consequences of bovine oocyte maturation, fertilization or early embryo development in vitro versus in vivo: implications for blastocyst yield and blastocyst quality. Mol. Reprod. Dev. 61, 234–248.
CrossRef | CAS | PubMed |

Russell, D. F., Baqir, S., Bordignon, J., and Betts, D. H. (2006). The impact of oocyte maturation media on early bovine embryonic development. Mol. Reprod. Dev. 73, 1255–1270.
CrossRef | CAS | PubMed |

Suzuki, H., Saito, Y., Kagawa, N., and Yang, X. (2003). In vitro fertilization and polyspermy in the pig: factors affecting fertilization rates and cytoskeletal reorganization of the oocyte. Microsc. Res. Tech. 61, 327–334.
CrossRef | PubMed |

Tajik, P., and Esfandabadi, N. S. (2003). In vitro maturation of caprine oocytes in different culture media. Small Rumin. Res. 47, 155–158.
CrossRef |

Tajik, P., Beheshti-Govij, R., Soleimani-Rad, J., Ghasemzadeh-Nava, H., and Mohamadi-Roshandeh, A. (2008). Effects of different concentrations of bovine follicular fluid and estrous cow serum on development of murine 2-cell embryos. Pak. J. Biol. Sci. 11, 628–632.
CrossRef | CAS | PubMed |

Tarín, J. J. (1996). Potential effects of age-associated oxidative stress on mammalian oocytes/embryos. Mol. Hum. Reprod. 2, 717–724.
CrossRef | PubMed |

Tarín, J. J., Trounson, A. O., and Sathananthan, H. (1999). Origin and ploidy of multipronuclear zygotes. Reprod. Fertil. Dev. 11, 273–279.
CrossRef | PubMed |

Ueno, S., Kurome, M., Ueda, H., Tomii, R., Hiruma, K., and Nagashima, H. (2005). Effects of maturation conditions on spindle morphology in porcine MII oocytes. J. Reprod. Dev. 51, 405–410.
CrossRef | PubMed |

Ulloa Ulloa, C. M., Yoshizawa, M., Komoriya, E., Mitsui, A., Nagai, T., and Kikuchi, K. (2008a). The blastocyst production rate and incidence of chromosomal abnormalities by developmental stage in in vitro produced porcine embryos. J. Reprod. Dev. 54, 22–29.
CrossRef |

Ulloa Ulloa, C. M., Yoshizawa, M., Yamashita, A., Hama, S., Mitsui, A., Hashi, C., Abe, H., Hoshi, H., Fukui, E., and Matsumoto, H. (2008b). Blastocyst production from in vitro-produced day-2 bovine embryos classified by cleavage stage, and cytogenetical evaluation of the resultant day-8 blastocysts. J. Reprod. Dev. 54, 465–472.
CrossRef | PubMed |

Van Soom, A., Vandaele, L., Goossens, K., de Kruif, A., and Peelman, L. (2007). Gamete origin in relation to early embryo development. Theriogenology 68, S131–S137.
CrossRef | CAS | PubMed |

Vialard, F., Boitrelle, F., Molina-Gomes, D., and Selva, J. (2011). Predisposition to aneuploidy in the oocyte. Cytogenet. Genome Res. 133, 127–135.
CrossRef | CAS | PubMed |

Villamediana, P., Vidal, F., and Paramio, M. T. (2001). Cytogenetic analysis of caprine 2- to 4-cell embryos produced in vitro. Zygote 9, 193–199.
CrossRef | CAS | PubMed |

Viuff, D., Rickords, L., Offenberg, H., Hyttel, P., Avery, B., Greve, T., Olsaker, I., Williams, J. L., Callesen, H., and Thomsen, P. D. (1999). A high proportion of bovine blastocysts produced in vitro are mixoploid. Biol. Reprod. 60, 1273–1278.
CrossRef | CAS | PubMed |

Wang, W. H., Hosoe, M., and Shioya, Y. (1997). Induction of cortical granule exocytosis of pig oocytes by spermatozoa during meiotic maturation. J. Reprod. Fertil. 109, 247–255.
CrossRef | CAS | PubMed |

Wortzman, G. B., and Evans, J. P. (2005). Membrane and cortical abnormalities in post-ovulatory aged eggs: analysis of fertilizability and establishment of the membrane block to polyspermy. Mol. Hum. Reprod. 11, 1–9.
CrossRef | PubMed |

Xu, L., Wang, Y., Zhou, P., Cao, Y. X., Huang, T. H., and Chian, R. C. (2008). Cytogenetic analysis of in vivo and in vitro matured oocytes derived from naturally cycling and stimulated mice. Syst. Biol. Reprod. Med. 54, 155–162.
CrossRef | CAS | PubMed |

Yang, X., Jiang, S., and Foote, R. H. (1993). Bovine oocyte development following different oocyte maturation and sperm capacitation procedures. Mol. Reprod. Dev. 34, 94–100.
CrossRef | CAS | PubMed |


   
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