CSIRO Publishing blank image blank image blank image blank imageBooksblank image blank image blank image blank imageJournalsblank image blank image blank image blank imageAbout Usblank image blank image blank image blank imageShopping Cartblank image blank image blank image You are here: Journals > Reproduction, Fertility and Development   
Reproduction, Fertility and Development
Journal Banner
  Vertebrate Reproductive Science & Technology
 
blank image Search
 
blank image blank image
blank image
 
  Advanced Search
   

Journal Home
About the Journal
Editorial Structure
Contacts
Content
Online Early
Current Issue
Just Accepted
All Issues
Special Issues
Research Fronts
Virtual Issues
Sample Issue
For Authors
General Information
Scope
Submit Article
Author Instructions
Open Access
Awards and Prizes
For Referees
Referee Guidelines
Review an Article
Annual Referee Index
For Subscribers
Subscription Prices
Customer Service
Print Publication Dates
Library Recommendation

blue arrow e-Alerts
blank image
Subscribe to our email Early Alert or RSS feeds for the latest journal papers.

red arrow Connect with us
blank image
facebook twitter logo LinkedIn

red arrow Connect with SRB
blank image
facebook TwitterIcon

Affiliated Societies

RFD is the official journal of the International Embryo Transfer Society and the Society for Reproductive Biology.


 

Article << Previous     |     Next >>   Contents Vol 22(1)

411 EFFICIENCY OF DAY SEVEN COLLECTION OF BOVINE EMBRYOS AFTER SUPEROVULATION BY FLUSHING THE OVIDUCTS AND THE UTERINE HORNS

V. Havlicek A, A. Kuzmany A, J. F. Beckers B, B. Remy B, G. Brem A, U. Besenfelder A

A University of Veterinary Medicine, Vienna, Austria;
B University of Liège, Belgium
 
 Export Citation
 Print
  


Abstract

Embryo collection on Day 7 following superovulation is characterized by wide variation in the number of ova/embryos recovered. The differences between the numbers of corpora lutea and recovered ova/embryos provide evidence for incomplete recovery. The aim of this study was to examine the effect of flushing only the uterine horns v. endoscopical flushing of both the oviducts and the uterine horns on embryo recovery rates. A total of 12 superovulated Simmental heifers, aged 18 to 24 months were presynchronized by i.m. administration of PGF2 (500 μg of cloprostenol; Estrumate®, Essex Tierarznei, Munich, Germany) twice within 11 days. Two days after each of the PGF2 treatments, the animals received 0.02 μg of GnRH (Receptal®, Intervet, Boxmeer, the Netherlands). Between Days 9 and 11 of the estrous cycle, the animals got the first of 8 consecutive, twice-daily FSH injections in decreasing doses (in total 300-400 mg of FSH equivalent according to body weight; Stimufol®, ULg FMV PhR, Tilman, Belgium). Two PGF2 treatments were administered 60 and 72 h after the initial FSH treatment. Finally, 48 h after the first PGF2 application, ovulation was induced by injecting 0.02 μg of GnRH simultaneously with the AI. The AI was performed by using 1 straw of semen from a Simmental bull of proven fertility. Seven days after estrus, the heifers were restrained and an epidural anesthesia was performed. The uterine horns of the heifers were first flushed nonsurgically (300 mL of PBS + 1% FCS). Additionally, the oviducts were flushed by a transvaginal endoscopic technique (50 mL of PBS+1% FCS) followed by repeated flushing (250 mL of PBS + 1% FCS) of the uterine horns (combined flushing). In total, 12 animals delivered 132 ova/embryos (mean = 11 ± 6.5; recovery rate = 83%), with 83 developed to blastocysts/morulae (mean = 6.9 ± 6.4). The first flush of the uterine horns gave 3.8 ova/embryos on average with 2.8 being blastocysts/morulae (developmental rate = 75.6%). The following combined flush gave 7.3 ova/embryos with 4.1 developed to blastocysts/morulae (developmental rate = 56.3%). No differences were found between flushing the left and right sides. In conclusion, the present study shows that the additional step involving flushing of oviducts plus uterine horns of superovulated heifers provided recovery of a higher number of ova/embryos. It is assumed that these extra numbers might be caused by the flushing of (1) the uterine horns repeatedly, (2) the region of the utero-tubal junctions, and (3) ova/embryos from the oviducts, probably as a result of incomplete migration.

   
    
Legal & Privacy | Contact Us | Help

CSIRO

© CSIRO 1996-2016