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Development of in-vitro-derived bovine embryos in protein-free media: effects of amino acids, glucose, pyruvate, lactate, phosphate and osmotic pressure

G. Wirtu A B F , C. E. Pope B , P. Damiani B , F. Miller C , B. L. Dresser B D , C. R. Short A , R. A. Godke A E and B. D. Bavister A D
+ Author Affiliations
- Author Affiliations

A Comparative Biomedical Sciences, Louisiana State University (LSU) School of Veterinary Medicine, Baton Rouge, LA 70803, USA.

B Audubon Nature Institute Center for Research of Endangered Species, New Orleans, LA 70131, USA.

C Cyagra Inc., Gibbon, NE 68840, USA.

D Department of Biological Sciences, University of New Orleans, LA 70148, USA.

E Department of Animal Sciences, LSU Agricultural Center, Baton Rouge, LA 70803, USA.

F To whom correspondence should be addressed. email: gwirtu1@lsu.edu

Reproduction, Fertility and Development 15(8) 439-449 https://doi.org/10.1071/RD03090
Submitted: 14 October 2003  Accepted: 9 February 2004   Published: 12 March 2004



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