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Effect of sperm pretreatment with sodium hydroxide and dithiothreitol on the efficiency of bovine intracytoplasmic sperm injection

M. E. Arias A , R. Sánchez A , J. Risopatrón A , L. Pérez A and R. Felmer A B C
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A Laboratorio de Reproducción, Centro de Biotecnología de La Reproducción (BIOREN-CEBIOR), Facultad de Medicina, Universidad de La Frontera, Montevideo 0870, PO box 54-D, Temuco, Chile.

B Departamento de Ciencias Agronómicas y Recursos Naturales, Facultad de Ciencias Agropecuarias y Forestales, Universidad de La Frontera, Montevideo 0870, PO box 54-D, Temuco, Chile.

C Corresponding author. Email: rfelmerd@gmail.com

Reproduction, Fertility and Development 26(6) 847-854 https://doi.org/10.1071/RD13009
Submitted: 21 January 2013  Accepted: 20 May 2013   Published: 18 June 2013



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