Prostaglandin and progesterone production by bovine luteal cells incubated in the presence or absence of the accessory cells of the corpus luteum and treated with interleukin-1β, indomethacin and luteinizing hormone

Abstract

This experiment examined production of prostaglandin (PG) F_2α , PGE₂ and progesterone by bovine luteal cells incubated with or without the accessory cells and treated with recombinant bovine interleukin-1β (10 ng), indomethacin (5 µg) or luteinizing hormone (50 ng). During pretreatment, progesterone production was similar in the luteal and luteal plus accessory cells, being greater in either of these than in accessory cells; PGF_2α was greatest in luteal plus accessory, intermediate in accessory and lowest in the luteal cells; PGE2 was similar among all cell arrangements. Luteinizing hormone or luteinizing hormone plus indomethacin stimulated progesterone in the luteal and luteal plus accessory cells, this being similar in these two cell arrangements. Interleukin-1β stimulated PGF_2α in luteal and luteal plus accessory cells, and tended to elevate PGF_2α in accessory cells. Interleukin-1β stimulated PGE₂ in all cell arrangements. Indomethacin inhibited the interleukin-1b-stimulated PGF_2α and PGE₂ release. Indomethacin or indomethacin plus luteinizing hormone inhibited basal PGE₂ in luteal and luteal plus accessory cells. These data suggest that progesterone production is not inßuenced by the presence of accessory cells of the corpus luteum, that accessory and luteal cells produce appreciable amounts of PG, and that recombinant bovine interleukin-1b stimulates PGE₂ and PGF_2α in the luteal and accessory cells.