Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
RESEARCH ARTICLE

Pregnancy recognition and conceptus implantation in domestic ruminants: roles of progesterone, interferons and endogenous retroviruses

Thomas E. Spencer A D , Greg A. Johnson B , Fuller W. Bazer A B , Robert C. Burghardt B and Massimo Palmarini C

A Center for Animal Biotechnology and Genomics, Department of Animal Science, Texas A&M University, College Station, TX 77843, USA.

B Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, TX 77843, USA.

C Institute of Comparative Medicine, University of Glasgow Veterinary School, Scotland, UK.

D Corresponding author. Email: tspencer@tamu.edu

Reproduction, Fertility and Development 19(1) 65-78 https://doi.org/10.1071/RD06102
Published: 12 December 2006

Abstract

The present review highlights new information on pregnancy recognition and conceptus development and implantation in sheep with respect to regulation by progesterone, interferons and endogenous retroviruses. After formation of the corpus luteum, progesterone acts on the endometrium and stimulates blastocyst growth and elongation to a filamentous conceptus (embryo/fetus and associated extra-embryonic membranes). The envelope of endogenous retroviruses related to Jaagsiekte sheep retroviruses appears to intrinsically regulate mononuclear trophectoderm cell proliferation and differentiation into trophoblast giant binucleate cells. The mononuclear trophectoderm cells of elongating sheep conceptuses secrete interferon-τ, which acts on the endometrium to prevent development of the luteolytic mechanism by inhibiting transcription of the gene for the oestrogen receptor α in the luminal and superficial ductal glandular epithelia. These actions prevent oestrogen-induced transcription of the oxytocin receptor gene and, therefore, oxytocin-induced luteolytic pulses of prostaglandin F. Progesterone downregulation of its receptors in luminal and glandular epithelia correlates temporally with a reduction in anti-adhesive mucin 1and induction of secreted galectin 15 (LGALS15) and secreted phosphoprotein 1, which are proposed to regulate trophectoderm proliferation and adhesion. Interferon-τ acts on the endometrial lumenal epithelium to induce WNT7A and to stimulate LGALS15, cathepsin L and cystatin C, which are candidate regulators of conceptus development and implantation. The number of potential contributors to maternal recognition and establishment of pregnancy continues to grow and this highlights our limited appreciation of the complexity of the key molecules and signal transduction pathways that intersect during these key developmental processes. The goal of improving reproductive efficiency by preventing embryonic losses that occur during the peri-implantation period of pregnancy in domestic ruminants provides the challenge to increase our knowledge of endometrial function and conceptus development.

Additional keywords: endogenous viruses related to Jaagsiekte sheep retroviruses, uterus.


Acknowledgments

The authors thank their colleagues and the present and past members of their laboratories who contributed to the research presented in this review. Owing to space limitations, many primary references could not be included in the manuscript. The authors’ work described herein was supported by National Research Initiative Competitive Grants 2001-02259 and 2005-35203-16252 from the USDA Cooperative State Research, Education and Extension Service, and NIH Grants HD32534 and P30 ES09106. M. Palmarini is a Wolfson-Royal Society research Merit awardee.


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