Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
RESEARCH ARTICLE

Formation of 5α-reduced androgens in the testes and urogenital tract of the grey short-tailed opossum, Monodelphis domestica

Jean D. Wilson A B C , Marilyn B. Renfree A , Richard J. Auchus B , Andrew J. Pask A and Geoffrey Shaw A
+ Author Affiliations
- Author Affiliations

A Department of Zoology, University of Melbourne, Vic. 3010, Australia.

B Department of Internal Medicine, University of Texas Southwestern Medical Center, Dallas, TX 75390-8857, USA.

C Corresponding author. Email: jwils1@mednet.swmed.edu

Reproduction, Fertility and Development 21(5) 649-654 https://doi.org/10.1071/RD08253
Submitted: 10 November 2008  Accepted: 20 March 2009   Published: 21 May 2009

Abstract

Testicular 5α-reduced androgens, largely 5α-androstane-3α,17β-diol (androstanediol), are responsible for virilisation of pouch young in one marsupial (the tammar wallaby), but are not formed until later in development in another marsupial (the brushtail possum) and in rodents. Because the mechanism of virilisation of the urogenital tract in the grey short-tailed opossum Monodelphis domestica has never been defined, androgen formation and metabolism were investigated in this species. Testis fragments from grey short-tailed opossums of a wide range of ages were incubated with [3H]-progesterone and the metabolites were separated by high-performance liquid chromatography (HPLC). The only 19-carbon metabolites identified in the youngest ages (5–26 days) and the major metabolites in adult testes were testosterone and androstenedione. At 30, 42 and 49 days of age, dihydrotestosterone and small amounts of androstanediol were present. Time-sequence studies indicated that dihydrotestosterone and androstanediol were formed from the 5α-reduction (and 3-keto reduction) of testosterone. In a second series of experiments, tissue fragments of a variety of urogenital tract tissues were incubated with [3H]-testosterone and the metabolites separated by HPLC. During the interval in which male urogenital tract differentiation takes place in this species (between Days 15 and 28), the major metabolite identified was dihydrotestosterone. We conclude that the timing of 5α-reductase expression in the testes of the grey short-tailed possum resembles that of rodents and the brushtail possum rather than that of the tammar wallaby and that dihydrotestosterone is probably the intracellular androgen responsible for virilisation of the urogenital tract in this species.


Acknowledgements

This study was supported by grant 208911 from the National Health and Medical Research Council of Australia (NHMRC) and by Grant I-1493 from the Robert A. Welsh Foundation. A.J.P. was supported by an NHMRC RD Wright Fellowship and M.B.R. was supported by an Australian Research Council Federation Fellowship. The authors thank C. David Vance for performing the HPLC of the samples and Professor Norman Saunders and Dr Joakim Ek for providing the opossums.


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