Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
RESEARCH ARTICLE

Colloid centrifugation removes seminal plasma and cholesterol from boar spermatozoa

R. Kruse A D , P. C. Dutta B and J. M. Morrell C E

A Clinical Sciences, Swedish University of Agricultural Sciences (SLU), Box 7054, SE-75007 Uppsala, Sweden.

B Department of Food Science, Uppsala BioCenter, Swedish University of Agricultural Sciences, Box 7051, SE-75007 Uppsala, Sweden.

C Division of Reproduction, Clinical Sciences, Swedish University of Agricultural Sciences, Box 7054, SE-75007 Uppsala, Sweden.

D Present address: Department of Clinical Medicine, School of Health and Medical Sciences, Örebro University, Örebro, Sweden.

E Corresponding author. Email: jane.morrell@slu.se

Reproduction, Fertility and Development 23(7) 858-865 http://dx.doi.org/10.1071/RD10260
Submitted: 7 October 2011  Accepted: 28 March 2011   Published: 19 August 2011

Abstract

The objective of the present study was to investigate the effect of Single-Layer Centrifugation (SLC) on boar spermatozoa, namely the effect of removal of seminal plasma proteins and cholesterol from the surface of spermatozoa. The presence of porcine seminal plasma proteins I and II (PSP-I/PSP-II) before and after SLC was studied using immunofluorescence, whereas the removal of cholesterol was shown qualitatively by thin-layer chromatography (TLC). Finally, the integrity of the sperm plasma membrane was observed by electron microscopy. It was shown that the seminal plasma proteins PSP-I and -II were removed from spermatozoa during SLC but could be restored by adding seminal plasma to the SLC-selected sperm samples. Some cholesterol was also lost from the spermatozoa during SLC but the plasma membrane itself appeared to be morphologically intact. Further studies are underway to examine the relevance of these findings to boar sperm cryopreservation and sperm fertility.

Additional keywords: PSP-I, PSP-II, SLC.


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