Cytoplasmic polyadenylation element binding protein 2 (CPEB2) is required for tight-junction assembly for establishment of porcine trophectoderm epithelium
Jeongwoo Kwon
A * , Shuha Park A * , Min-Jung Seong A , Inchul Choi B C and Nam-Hyung Kim A C
A Department of Animal Sciences, Chungbuk National University, Naesudong-ro, Seowon-gu, Cheongju-si, Chungcheongbuk-do, 28864, Republic of Korea.
B Department of Animal and Dairy Sciences, College of Agriculture and Life Sciences, Chungnam National University, Korea, 34134, Republic of Korea.
C Corresponding authors. Emails: nhkim@chungbuk.ac.kr; icchoi@cnu.ac.kr
Reproduction, Fertility and Development 31(2) 412-419 https://doi.org/10.1071/RD18098
Submitted: 13 March 2018 Accepted: 27 July 2018 Published: 27 August 2018
Abstract
Cytoplasmic polyadenylation element binding protein (CPEB) is an RNA-binding protein that promotes elongation of poly(A) tails and regulates mRNA translation. CPEB depletion in mammary epithelium is known to disrupt tight-junction (TJ) assembly via mislocalisation of tight junction protein 1 (TJP1), but the role of CPEB in the biological functions associated with TJs has not yet been studied. The objective of this study was to investigate the roles of CPEB2 during porcine parthenote development. CPEB2 was detected in both the nuclei and apical cytoplasm at the 4- and 8-cell stages and was localised to cell–cell contact after the initiation of the morula stage. Its depletion led to retarded blastocyst formation caused by impaired TJ assembly. Moreover, transcription of TJ-associated genes, including TJP1, Coxsackie virus and adenovirus receptor (CXADR) and occludin (OCLN), was not affected, but the corresponding proteins were not properly localised at the apical cell membrane in morulae, suggesting that CPEB2 confers mRNA stability or determines subcellular localisation for translation. Remarkably reduced relative levels of TJP1 transcripts bearing the 3′-untranslated region were noted, indicating that CPEB2 mediates TJP1 mRNA stability. In conclusion, our findings demonstrate that because of its regulation of TJP1, CPEB2 is required for TJ assembly during porcine blastocyst development.
Additional keywords: blastocyst formation, porcine parthenote, ZO-1.
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