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RESEARCH ARTICLE
Contents Vol 73(8)

‘Live and Large’: Super-Resolution Optical Fluctuation Imaging (SOFI) and Expansion Microscopy (ExM) of Microtubule Remodelling by Rabies Virus P Protein

Ashley M. Rozario A , Fabian Zwettler B , Sam Duwé C , Riley B. Hargeaves A , Aaron Brice D , Peter Dedecker C , Markus Sauer B , Gregory W. Moseley D , Donna R. Whelan E and Toby D. M. Bell https://orcid.org/0000-0002-4570-5595 A F
+ Author Affiliations
- Author Affiliations

A School of Chemistry, Monash University, Melbourne, Vic. 3800, Australia.

B Department of Biotechnology and Biophysics, Biocenter, University of Würzburg, 10 Am Hubland, 97074 Würzburg, Germany.

C Department of Chemistry, KU Leuven, Celestijnenlaan 200G, Leuven 3001, Belgium.

D Monash Biomedicine Discovery Institute, Department of Microbiology, Monash University, Melbourne, Vic. 3800, Australia.

E La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Vic. 3552, Australia.

F Corresponding author. Email: toby.bell@monash.edu

Australian Journal of Chemistry 73(8) 686-692 https://doi.org/10.1071/CH19571
Submitted: 3 November 2019  Accepted: 13 January 2020   Published: 27 April 2020

Abstract

The field of super-resolution microscopy continues to progress rapidly, both in terms of evolving techniques and methodologies as well as in the development of new multi-disciplinary applications. Two current drivers of innovation are increasing the possible resolution gain and application in live samples. Super-resolution optical fluctuation imaging (SOFI) is well suited to live samples while expansion microscopy (ExM) enables obtainment of sub-diffraction information via conventional imaging. In this Highlight we provide a brief outline of these methods and report results from application of SOFI and ExM in our on-going study into microtubule remodelling by rabies virus P proteins. We show that MT bundles in live cells transfected with rabies virus P3 protein can be visualised using SOFI in a time-lapse fashion for up to half an hour and can be expanded using current Pro-ExM protocols and imaged using conventional microscopy.


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