Hepatitis B virus surface protein induces oxidative stress by increasing peroxides and inhibiting antioxidant defences in human spermatozoa
Lin Cheng A B C * , Pingnan Sun A B C * , Xiaoling Xie A B C , Dongmei Sun D , Qi Zhou A B C , Shaozhe Yang A B C , Qingdong Xie A B C and Xiaoling Zhou
A B C E
+ Author Affiliations
- Author Affiliations
A Stem Cell Research Center, Shantou University Medical College, Shantou 515041, PR China.
B Research Center for Reproductive Medicine, Shantou University Medical College, Shantou 515041, PR China.
C Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular Immunopathology, Shantou University Medical College, Shantou 515041, PR China.
D Shenzhen Longgang District Maternity & Child Healthcare Hospital, Shenzhen 518172, PR China.
E Corresponding author. Email: xlzhou@stu.edu.cn
Reproduction, Fertility and Development 32(14) 1180-1189 https://doi.org/10.1071/RD20130
Submitted: 16 May 2020 Accepted: 10 August 2020 Published: 1 October 2020
Journal Compilation © CSIRO 2020 Open Access CC BY-NC-ND
Abstract
Hepatitis B virus (HBV) infection may affect sperm motility in patients with HBV. HBV surface protein (HBs) decreases mitochondrial membrane potential, impairs motility and induces apoptotic-like changes in human spermatozoa. However, little is known about how human spermatozoa respond to reactive oxygen species (ROS; mainly peroxides) induced by HBs. In this study, HBs induced supraphysiological ROS levels in human spermatozoa and reduced the formation of 2-cell embryos (obtained from hamster oocytes and human spermatozoa). HBs induced a pre-apoptotic status in human spermatozoa, as well as antioxidant defences by increasing glutathione peroxidase 4 (GPX4) and peroxiredoxin 5 (PRDX5) levels. These results highlight the molecular mechanism responsible for the oxidative stress in human spermatozoa exposed to HBV and the antioxidant defence response involving GPX4 and PRDX5.
Keywords: 2-cell embryo, glutathione peroxidase 4 (GPX4), peroxiredoxin 5 (PRDX5).
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