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Ecology, management and conservation in natural and modified habitats
REVIEW (Open Access)

Origins and population genetics of sambar deer (Cervus unicolor) introduced to Australia and New Zealand

Lee A. Rollins https://orcid.org/0000-0002-3279-7005 A B * , Daniel Lees https://orcid.org/0000-0002-5214-2727 B , Andrew P. Woolnough C D , Andrea J. West B , Michael Perry E and David M. Forsyth https://orcid.org/0000-0001-5356-9573 A F
+ Author Affiliations
- Author Affiliations

A Evolution & Ecology Research Centre, School of Biological, Earth and Environmental Sciences, University of New South Wales, Sydney, NSW 2052, Australia.

B Centre for Integrative Ecology, School of Life and Environmental Sciences, Deakin University, Locked Bag 20000, Geelong, Vic. 3216, Australia.

C Department of Jobs, Precincts and Regions, 121 Exhibition Street, Melbourne, Vic. 3000, Australia.

D Research, Innovation and Commercialisation, The University of Melbourne, Parkville, Vic. 3010, Australia.

E Department of Conservation, 59 Marine Parade, Napier 4110, New Zealand.

F Vertebrate Pest Research Unit, New South Wales Department of Primary Industries, 1447 Forest Road, Orange, NSW 2800, Australia.

* Correspondence to: l.rollins@unsw.edu.au

Handling Editor: Tony Pople

Wildlife Research 50(9) 716-727 https://doi.org/10.1071/WR22120
Submitted: 7 July 2022  Accepted: 22 May 2023   Published: 20 July 2023

© 2023 The Author(s) (or their employer(s)). Published by CSIRO Publishing. This is an open access article distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License (CC BY-NC-ND)

Abstract

Context: Some populations of introduced species cause significant undesirable impacts but can also act as reservoirs for genetic diversity. Sambar deer (Cervus unicolor) are ‘Vulnerable’ in their native range and invasive in Australia and New Zealand. Genetic data can be used to determine whether these introduced populations might serve as genetic reservoirs for declining native populations and to identify spatial units for management.

Aims: We aimed to identify the provenance of sambar deer in Australia and New Zealand, and to characterise their genetic diversity and population structure.

Methods: We used mitochondrial control region sequences and 18 nuclear microsatellite loci of 24 New Zealand and 63 Australian sambar deer collected across continuous habitat in each location. We estimated genetic diversity and population differentiation by using pairwise FST, AMOVA, and Structure analyses. We compared our data with 27 previously published native and invasive range sequences to identify phylogenetic relationships.

Key results: Sambar deer in Australia and New Zealand are genetically more similar to those in the west of the native range (South and Central Highlands of India, and Sri Lanka), than to those in the east (eastern India, and throughout Southeast Asia). Nuclear genetic diversity was lower than in the native range; only one mitochondrial haplotype was found in each introduced population. Australian and New Zealand sambar deer were genetically distinct but there was no population structure within either population.

Conclusions: The genetic differences we identified between these two introduced populations at putatively neutral loci indicate that there also may be underlying diversity at functional loci. The lack of population genetic structure that we found within introduced populations suggests that individuals within these populations do not experience barriers to dispersal across the areas sampled.

Implications: Although genetic diversity is reduced in the introduced range compared with the native range, sambar deer in Australia and New Zealand harbour unique genetic variants that could be used to strengthen genetic diversity in populations under threat in the native range. The apparent high levels of gene flow across the areas we sampled suggest that localised control is unlikely to be effective in Australia and New Zealand.

Keywords: Cervidae, invasive species, management units, microsatellite, mitochondrial DNA, population genetics, Rusa unicolor, sambar deer.


References

Allendorf FW, Luikart G (2007) ‘Conservation and the genetics of populations.’ (Blackwell: Malden, MA, USA)

Alvarez, I, Fernandez, I, Traore, A, Menendez-Arias, NA, and Goyache, F (2021). Population structure assessed using microsatellite and SNP data: an empirical comparison in West African cattle. Animals (Basel) 11, 151.
Population structure assessed using microsatellite and SNP data: an empirical comparison in West African cattle.Crossref | GoogleScholarGoogle Scholar |

Armstrong, DP, and Seddon, PJ (2008). Directions in reintroduction biology. Trends in Ecology & Evolution 23, 20–25.
Directions in reintroduction biology.Crossref | GoogleScholarGoogle Scholar |

Balakrishnan, CN, Monfort, SL, Gaur, A, Singh, L, and Sorenson, MD (2003). Phylogeography and conservation genetics of Eld’s deer (Cervus eldi). Molecular Ecology 12, 1–10.
Phylogeography and conservation genetics of Eld’s deer (Cervus eldi).Crossref | GoogleScholarGoogle Scholar |

Bandelt, HJ, Forster, P, and Rohl, A (1999). Median-joining networks for inferring intraspecific phylogenies. Molecular Biology and Evolution 16, 37–48.
Median-joining networks for inferring intraspecific phylogenies.Crossref | GoogleScholarGoogle Scholar |

Banwell DB (2006) ‘The rusa, sambar and whitetail deer. New Zealand Big Game Records Series. Vol. 4.’ (Halcyon Press)

Bengsen, AJ, Gentle, MN, Mitchell, JL, Pearson, HE, and Saunders, GR (2014). Impacts and management of wild pigs Sus scrofa in Australia. Mammal Review 44, 135–147.
Impacts and management of wild pigs Sus scrofa in Australia.Crossref | GoogleScholarGoogle Scholar |

Bennett, A, Haydon, S, Stevens, M, and Coulson, G (2015). Culling reduces fecal pellet deposition by introduced sambar (Rusa unicolor) in a protected water catchment. Wildlife Society Bulletin 39, 268–275.
Culling reduces fecal pellet deposition by introduced sambar (Rusa unicolor) in a protected water catchment.Crossref | GoogleScholarGoogle Scholar |

Bentley A (1998) ‘An introduction to the deer of Australia.’ Bunyip edn. (Australian Deer Research Association: Melbourne, Vic., Australia)

Bomford, M, and O’Brien, P (1995). Eradication or control for vertebrate pests? Wildlife Society Bulletin 23, 249–255.

Bonizzoni, M, Guglielmino, CR, Smallridge, CJ, Gomulski, M, Malacrida, AR, and Gasperi, G (2004). On the origins of medfly invasion and expansion in Australia. Molecular Ecology 13, 3845–3855.
On the origins of medfly invasion and expansion in Australia.Crossref | GoogleScholarGoogle Scholar |

Bradshaw, CJA, Isagi, Y, Kaneko, S, Bowman, DMJS, and Brook, BW (2006). Conservation value of non-native banteng in northern Australia. Conservation Biology 20, 1306–1311.
Conservation value of non-native banteng in northern Australia.Crossref | GoogleScholarGoogle Scholar |

Byrne, PG, and Silla, AJ (2020). An experimental test of the genetic consequences of population augmentation in an amphibian. Conservation Science and Practice 2, e194.
An experimental test of the genetic consequences of population augmentation in an amphibian.Crossref | GoogleScholarGoogle Scholar |

Catchpole, EA, Fan, Y, Morgan, BJT, Clutton-Brock, TH, and Coulson, T (2004). Sexual dimorphism, survival and dispersal in red deer. Journal of Agricultural, Biological, and Environmental Statistics 9, 1–26.
Sexual dimorphism, survival and dispersal in red deer.Crossref | GoogleScholarGoogle Scholar |

Chan, WY, Peplow, LM, Menéndez, P, Hoffmann, AA, and Van Oppen, MJH (2018). Interspecific hybridization may provide novel opportunities for coral reef restoration. Frontiers in Marine Science 5, 160.
Interspecific hybridization may provide novel opportunities for coral reef restoration.Crossref | GoogleScholarGoogle Scholar |

Comte, S, Thomas, E, Bengsen, AJ, Bennett, A, Davis, NE, Brown, D, and Forsyth, DM (2023). Cost-effectiveness of volunteer and contract ground-based shooting of sambar deer in Australia. Wildlife Research , .
Cost-effectiveness of volunteer and contract ground-based shooting of sambar deer in Australia.Crossref | GoogleScholarGoogle Scholar |

Côté, SD, Rooney, TP, Tremblay, J-P, Dussault, C, and Waller, DM (2004). Ecological impacts of deer overabundance. Annual Review of Ecology, Evolution, and Systematics 35, 113–147.
Ecological impacts of deer overabundance.Crossref | GoogleScholarGoogle Scholar |

Coulon, A, Fitzpatrick, JW, Bowman, R, Stith, BM, Makarewich, CA, Stenzler, LM, and Lovette, IJ (2008). Congruent population structure inferred from dispersal behaviour and intensive genetic surveys of the threatened Florida scrub-jay (Aphelocoma coerulescens). Molecular Ecology 17, 1685–1701.
Congruent population structure inferred from dispersal behaviour and intensive genetic surveys of the threatened Florida scrub-jay (Aphelocoma coerulescens).Crossref | GoogleScholarGoogle Scholar |

Cowled, BD, Elsworth, P, and Lapidge, SJ (2008). Additional toxins for feral pig (Sus scrofa) control: identifying and testing Achilles’ heels. Wildlife Research 35, 651–662.
Additional toxins for feral pig (Sus scrofa) control: identifying and testing Achilles’ heels.Crossref | GoogleScholarGoogle Scholar |

Davies, C, Wright, W, Wedrowicz, F, and Hogan, FE (2020). A DNA toolbox for non-invasive genetic studies of sambar deer (Rusa unicolor). Australian Mammalogy 42, 58–66.
A DNA toolbox for non-invasive genetic studies of sambar deer (Rusa unicolor).Crossref | GoogleScholarGoogle Scholar |

Davies, C, Wright, W, Wedrowicz, F, Pacioni, C, and Hogan, FE (2022). Delineating genetic management units of sambar deer (Rusa unicolor) in south-eastern Australia, using opportunistic tissue sampling and targeted scat collection. Wildlife Research 49, 147–157.
Delineating genetic management units of sambar deer (Rusa unicolor) in south-eastern Australia, using opportunistic tissue sampling and targeted scat collection.Crossref | GoogleScholarGoogle Scholar |

Davis, NE, Bennett, A, Forsyth, DM, Bowman, DMJS, Lefroy, EC, Wood, SW, Woolnough, AP, West, P, Hampton, JO, and Johnson, CN (2016). A systematic review of the impacts and management of introduced deer (family Cervidae) in Australia. Wildlife Research 43, 515–532.
A systematic review of the impacts and management of introduced deer (family Cervidae) in Australia.Crossref | GoogleScholarGoogle Scholar |

Dlugosch, KM, and Parker, IM (2008). Founding events in species invasions: genetic variation, adaptive evolution, and the role of multiple introductions. Molecular Ecology 17, 431–449.
Founding events in species invasions: genetic variation, adaptive evolution, and the role of multiple introductions.Crossref | GoogleScholarGoogle Scholar |

Donne TE (1924) ‘The game animals of New Zealand: an account of their introduction, acclimatization and development.’ (J. Murray)

Downes M (1983) ‘The Forest Deer Project, 1982: a report to the Forests Commission Victoria. Vol. 2.’ (Australian Deer Research Foundation for the Australian Deer Association: Melbourne, Vic., Australia)

Dunn, J (1985). Sambar deer in the Kosciusko National Park. Australian Deer 10, 3–5.

Evanno, G, Regnaut, S, and Goudet, J (2005). Detecting the number of clusters of individuals using the software structure: a simulation study. Molecular Ecology 14, 2611–2620.
Detecting the number of clusters of individuals using the software structure: a simulation study.Crossref | GoogleScholarGoogle Scholar |

Excoffier, L, Smouse, PE, and Quattro, JM (1992). Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131, 479–491.
Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data.Crossref | GoogleScholarGoogle Scholar |

Excoffier, L, Laval, G, and Schneider, S (2005). Arlequin (version 3.0): an integrated software package for population genetics data analysis. Evolutionary Bioinformatics Online 1, 47–50.

Fahey B (2017) Tools for managing wild deer: fencing. In ‘2016 National Wild Deer Management Workshop Proceedings’, Adelaide, 17−18 November 2016. (Eds D Forsyth, T Pople, B Page, A Moriarty, D Ramsey, J Parkes, A Wiebkin, C Lane). (Invasive Animals Cooperative Research Centre: Canberra, ACT, Australia)

Falush, D, Stephens, M, and Pritchard, JK (2003). Inference of population structure using multilocus genotype data: linked loci and correlated allele frequencies. Genetics 164, 1567–1587.
Inference of population structure using multilocus genotype data: linked loci and correlated allele frequencies.Crossref | GoogleScholarGoogle Scholar |

Forsyth, DM, and Davis, NE (2011). Diets of non-native deer in Australia estimated by macroscopic versus microhistological rumen analysis. The Journal of Wildlife Management 75, 1488–1497.
Diets of non-native deer in Australia estimated by macroscopic versus microhistological rumen analysis.Crossref | GoogleScholarGoogle Scholar |

Forsyth DM, Stamation KA, Woodford L (2015) Distributions of Sambar Deer, Rusa Deer and Sika Deer in Victoria. Arthur Rylah Institute for Environmental Research Unpublished Client Report for the Biosecurity Branch, Department of Economic Development, Jobs, Transport and Resources. Arthur Rylah Institute for Environmental Research, Department of Environment, Land, Water and Planning, Melbourne, Vic., Australia.

Frankham, R, Lees, K, Montgomery, ME, England, PR, Lowe, EH, and Briscoe, DA (1999). Do population size bottlenecks reduce evolutionary potential? Animal Conservation 2, 255–260.
Do population size bottlenecks reduce evolutionary potential?Crossref | GoogleScholarGoogle Scholar |

Frankham, R, Ballou, JD, Eldridge, MDB, Lacy, RC, Ralls, K, Dudash, MR, and Fenster, CB (2011). Predicting the probability of outbreeding depression. Conservation Biology 25, 465–475.
Predicting the probability of outbreeding depression.Crossref | GoogleScholarGoogle Scholar |

Fraser K, Nugent G (2005) Sambar deer. In ‘The handbook of New Zealand mammals’. (Ed. CM King) pp. 436–442. (Oxford University Press)

Fraser, KW, Cone, JM, and Whitford, EJ (2000). A revision of the established ranges and new populations of 11 introduced ungulate species in New Zealand. Journal of the Royal Society of New Zealand 30, 419–437.
A revision of the established ranges and new populations of 11 introduced ungulate species in New Zealand.Crossref | GoogleScholarGoogle Scholar |

Gormley, AM, Forsyth, DM, Griffioen, P, Lindeman, M, Ramsey, DSL, Scroggie, MP, and Woodford, L (2011). Using presence-only and presence–absence data to estimate the current and potential distributions of established invasive species. Journal of Applied Ecology 48, 25–34.
Using presence-only and presence–absence data to estimate the current and potential distributions of established invasive species.Crossref | GoogleScholarGoogle Scholar |

Goudet J (2002) FSTAT, a program to estimate and test gene diversities and fixation indices version 2.9.3.2. Available from http://www2.unil.ch/popgen/softwares/fstat.htm [updated from Goudet (1995)])

Gupta SK (2014) Assessment of genetic variation in sambar deer (Rusa unicolor). Saurashtra University, Rajkot, Gujarat, India.

Gupta, SK, Kumar, A, Gaur, A, and Hussain, SA (2015). Detection of 40 bp insertion-deletion (INDEL) in mitochondrial control region among sambar (Rusa unicolor) populations in India. BMC Research Notes 8, 581.
Detection of 40 bp insertion-deletion (INDEL) in mitochondrial control region among sambar (Rusa unicolor) populations in India.Crossref | GoogleScholarGoogle Scholar |

Hampton, JO, Spencer, PBS, Alpers, DL, Twigg, LE, Woolnough, AP, Doust, J, Higgs, T, and Pluske, J (2004). Molecular techniques, wildlife management and the importance of genetic population structure and dispersal: a case study with feral pigs. Journal of Applied Ecology 41, 735–743.
Molecular techniques, wildlife management and the importance of genetic population structure and dispersal: a case study with feral pigs.Crossref | GoogleScholarGoogle Scholar |

Harris LH (1966) ‘Hunting sambar deer.’ (New Zealand Forest Service)

Harris, LH (1971). Notes on the introduction and history of sambar deer in New Zealand. New Zealand Wildlife 35, 33–42.

Hill, E, Linacre, A, Toop, S, Murphy, N, and Strugnell, J (2019). Widespread hybridization in the introduced hog deer population of Victoria, Australia, and its implications for conservation. Ecology and Evolution 9, 10828–10842.
Widespread hybridization in the introduced hog deer population of Victoria, Australia, and its implications for conservation.Crossref | GoogleScholarGoogle Scholar |

Hill, E, Murphy, N, Li-Williams, S, Davies, C, Forsyth, DM, Comte, S, Rollins, LA, Hogan, F, Wedrowicz, F, Crittle, T, Thomas, E, Woodford, L, and Pacioni, C (2023). Hybridisation rates, population structure, and dispersal of sambar deer (Cervus unicolor) and rusa deer (Cervus timorensis) in south-eastern Australia. Wildlife Research , .

Hoffmann, AA, Miller, AD, and Weeks, AR (2021). Genetic mixing for population management: From genetic rescue to provenancing. Evolutionary Applications 14, 634–652.
Genetic mixing for population management: From genetic rescue to provenancing.Crossref | GoogleScholarGoogle Scholar |

Hufbauer, RA, Szűcs, M, Kasyon, E, Youngberg, C, Koontz, MJ, Richards, C, Tuff, T, and Melbourne, BA (2015). Three types of rescue can avert extinction in a changing environment. Proceedings of the National Academy of Sciences 112, 10557–10562.

Jackson S, Jackson SM, Groves C (2015) ‘Taxonomy of Australian mammals.’ (CSIRO Publishing)

Kjellander, P, Hewison, AJM, Liberg, O, Angibault, J-M, Bideau, E, and Cargnelutti, B (2004). Experimental evidence for density-dependence of home-range size in roe deer (Capreolus capreolus L.): a comparison of two long-term studies. Oecologia 139, 478–485.
Experimental evidence for density-dependence of home-range size in roe deer (Capreolus capreolus L.): a comparison of two long-term studies.Crossref | GoogleScholarGoogle Scholar |

Kolbe, JJ, Glor, RE, Rodriguez Schettino, L, Lara, AC, Larson, A, and Losos, JB (2004). Genetic variation increases during biological invasion by a Cuban lizard. Nature 431, 177–181.
Genetic variation increases during biological invasion by a Cuban lizard.Crossref | GoogleScholarGoogle Scholar |

Larkin, MA, Blackshields, G, Brown, NP, Chenna, R, McGettigan, PA, McWilliam, H, Valentin, F, Wallace, IM, Wilm, A, Lopez, R, Thompson, JD, Gibson, TJ, and Higgins, DG (2007). Clustal W and Clustal X version 2.0. Bioinformatics 23, 2947–2948.
Clustal W and Clustal X version 2.0.Crossref | GoogleScholarGoogle Scholar |

Latham, ADM, Warburton, B, Byrom, AE, and Pech, RP (2017). The ecology and management of mammal invasions in forests. Biological Invasions 19, 3121–3139.
The ecology and management of mammal invasions in forests.Crossref | GoogleScholarGoogle Scholar |

Lee, CE (2002). Evolutionary genetics of invasive species. Trends in Ecology & Evolution 17, 386–391.
Evolutionary genetics of invasive species.Crossref | GoogleScholarGoogle Scholar |

Leslie, DM (2011). Rusa unicolor (Artiodactyla: Cervidae). Mammalian Species 43, 1–30.
Rusa unicolor (Artiodactyla: Cervidae).Crossref | GoogleScholarGoogle Scholar |

Librado, P, and Rozas, J (2009). DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics 25, 1451–1452.
DnaSP v5: a software for comprehensive analysis of DNA polymorphism data.Crossref | GoogleScholarGoogle Scholar |

Lindeman MJ, Forsyth DM (2008) Agricultural impacts of wild deer in Victoria. Heidelberg: Arthur Rylah Institute for Environmental Research, Department of Sustainability and Environment, Melbourne, Vic., Australia.

Loe, LE, Mysterud, A, Veiberg, V, and Langvatn, R (2009). Negative density-dependent emigration of males in an increasing red deer population. Proceedings of the Royal Society of London B: Biological Sciences 276, 2581–2597.
Negative density-dependent emigration of males in an increasing red deer population.Crossref | GoogleScholarGoogle Scholar |

Longmire, JL, Lewis, AK, Brown, NC, Buckingham, JM, Clark, LM, Jones, MD, Meincke, LJ, Meyne, J, Ratliff, RL, Ray, FA, Wagner, RP, and Moyzis, RK (1988). Isolation and molecular characterization of a highly polymorphic centromeric tandem repeat in the family falconidae. Genomics 2, 14–24.
Isolation and molecular characterization of a highly polymorphic centromeric tandem repeat in the family falconidae.Crossref | GoogleScholarGoogle Scholar |

Martins, RF, Schmidt, A, Lenz, D, Wilting, A, and Fickel, J (2018). Human-mediated introduction of introgressed deer across Wallace’s line: historical biogeography of Rusa unicolor and R. timorensis. Ecology and Evolution 8, 1465–1479.
Human-mediated introduction of introgressed deer across Wallace’s line: historical biogeography of Rusa unicolor and R. timorensis.Crossref | GoogleScholarGoogle Scholar |

Mattioli S (2011) Family Cervidae (Deer). In ‘Handbook of the mammals of the world. Vol. 2’. (Eds Wilson DE, Mittermeier RA) pp. 350–443. (Lynx Editions)

Moloney, PD, Gormley, AM, Toop, SD, Flesch, JS, Forsyth, DM, Ramsey, DSL, and Hampton, JO (2022). Bayesian modelling reveals differences in long-term trends in the harvest of native and introduced species by recreational hunters in Australia. Wildlife Research 49, 673–685.
Bayesian modelling reveals differences in long-term trends in the harvest of native and introduced species by recreational hunters in Australia.Crossref | GoogleScholarGoogle Scholar |

Moriarty, A (2004). The liberation, distribution, abundance and management of wild deer in Australia. Wildlife Research 31, 291–299.
The liberation, distribution, abundance and management of wild deer in Australia.Crossref | GoogleScholarGoogle Scholar |

Nei, M (1972). Genetic distance between populations. The American Naturalist 106, 283–292.
Genetic distance between populations.Crossref | GoogleScholarGoogle Scholar |

Neilan, BA, Wilton, AN, and Jacobs, D (1997). A universal procedure for primer labelling of amplicons. Nucleic Acids Research 25, 2938–2939.
A universal procedure for primer labelling of amplicons.Crossref | GoogleScholarGoogle Scholar |

Nugent G, Forsyth DM, Latham ADM, Speedy C, Allen RB, Asher GW, Tustin KG (2021). Family Cervidae. In ‘The handbook of New Zealand mammals’. (Eds CM King, DM Forsyth) pp. 447–509. (CSIRO Publishing: Melbourne, Vic., Australia)

Parkes, JP (1990). Eradication of feral goats on islands and habitat islands. Journal of the Royal Society of New Zealand 20, 297–304.
Eradication of feral goats on islands and habitat islands.Crossref | GoogleScholarGoogle Scholar |

Parkes, JP, Ramsey, DSL, Macdonald, N, Walker, K, McKnight, S, Cohen, BS, and Morrison, SA (2010). Rapid eradication of feral pigs (Sus scrofa) from Santa Cruz Island, California. Biological Conservation 143, 634–641.
Rapid eradication of feral pigs (Sus scrofa) from Santa Cruz Island, California.Crossref | GoogleScholarGoogle Scholar |

Peakall, R, and Smouse, PE (2006). GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research. Molecular Ecology Notes 6, 288–295.
GENALEX 6: genetic analysis in Excel. Population genetic software for teaching and research.Crossref | GoogleScholarGoogle Scholar |

Pritchard JK, Wen X, Falush D (2007) Documentation for structure software: version 2.2. Available at https://web.stanford.edu/group/pritchardlab/structure.html

Randi, E, Mucci, N, Claro-Hergueta, F, Bonnet, A, and Douzery, EJP (2001). A mitochondrial DNA control region phylogeny of the Cervinae: speciation in Cervus and implications for conservation. Animal Conservation 4, 1–11.
A mitochondrial DNA control region phylogeny of the Cervinae: speciation in Cervus and implications for conservation.Crossref | GoogleScholarGoogle Scholar |

Ripple, WJ, Newsome, TM, Wolf, C, Dirzo, R, Everatt, KT, Galetti, M, Hayward, MW, Kerley, GIH, Levi, T, Lindsey, PA, Macdonald, DW, Malhi, Y, Painter, LE, Sandom, CJ, Terborgh, J, and Van Valkenburgh, B (2015). Collapse of the world’s largest herbivores. Science Advances 1, e1400103.
Collapse of the world’s largest herbivores.Crossref | GoogleScholarGoogle Scholar |

Robertson, BC, and Gemmell, NJ (2004). Defining eradication units to control invasive pests. Journal of Applied Ecology 41, 1042–1048.
Defining eradication units to control invasive pests.Crossref | GoogleScholarGoogle Scholar |

Rollins, LA, Woolnough, AP, and Sherwin, WB (2006). Population genetic tools for pest management: a review. Wildlife Research 33, 251–261.
Population genetic tools for pest management: a review.Crossref | GoogleScholarGoogle Scholar |

Rollins, LA, Woolnough, AP, Wilton, AN, Sinclair, R, and Sherwin, WB (2009). Invasive species can’t cover their tracks: using microsatellites to assist management of starling (Sturnus vulgaris) populations in Western Australia. Molecular Ecology 18, 1560–1573.
Invasive species can’t cover their tracks: using microsatellites to assist management of starling (Sturnus vulgaris) populations in Western Australia.Crossref | GoogleScholarGoogle Scholar |

Rousset, F (2008). GENEPOP’007: a complete re-implementation of the GENEPOP software for Windows and Linux. Molecular Ecology Resources 8, 103–106.
GENEPOP’007: a complete re-implementation of the GENEPOP software for Windows and Linux.Crossref | GoogleScholarGoogle Scholar |

Seutin, G, White, BN, and Boag, PT (1991). Preservation of avian blood and tissue samples for DNA analyses. Canadian Journal of Zoology 69, 82–90.
Preservation of avian blood and tissue samples for DNA analyses.Crossref | GoogleScholarGoogle Scholar |

Shailer L (1957) ‘The sambar deer.’ (New Zealand Deerstalkers Association Inc.: Wellington, New Zealand)

Signorile, AL, Reuman, DC, Lurz, PWW, Bertolino, S, Carbone, C, and Wang, J (2016). Using DNA profiling to investigate human-mediated translocations of an invasive species. Biological Conservation 195, 97–105.
Using DNA profiling to investigate human-mediated translocations of an invasive species.Crossref | GoogleScholarGoogle Scholar |

Spencer, PBS, Giustiniano, D, Hampton, JO, Gee, P, Burrows, N, Rose, K, Martin, GR, and Woolnough, AP (2012). Identification and management of a single large population of wild dromedary camels. The Journal of Wildlife Management 76, 1254–1263.
Identification and management of a single large population of wild dromedary camels.Crossref | GoogleScholarGoogle Scholar |

Sunde, J, Yilderim, Y, Tibblin, P, and Forsman, A (2020). Comparing the performance of microsatellites and RADseq in population genetic studies: analysis of data for pike (Esox lucius) and a synthesis of previous studies. Frontiers in Genetics 11, 218.
Comparing the performance of microsatellites and RADseq in population genetic studies: analysis of data for pike (Esox lucius) and a synthesis of previous studies.Crossref | GoogleScholarGoogle Scholar |

Timmins R, Kawanishi K, Giman B, Lynam A, Chan B, Steinmetz R, Sagar Baral H, Samba Kumar N (2015) Rusa unicolor (errata version published in 2015). The IUCN Red List of Threatened Species 2015: e.T41790A85628124. Available at http://dx.doi.org/10.2305/IUCN.UK.2015-2.RLTS.T41790A22156247.en

Tsutsui, ND, Suarez, AV, Holway, DA, and Case, TJ (2000). Reduced genetic variation and the success of an invasive species. Proceedings of the National Academy of Sciences 97, 5948–5953.
Reduced genetic variation and the success of an invasive species.Crossref | GoogleScholarGoogle Scholar |

Wallach, AD, Ripple, WJ, and Carroll, SP (2015). Novel trophic cascades: apex predators enable coexistence. Trends in Ecology & Evolution 30, 146–153.
Novel trophic cascades: apex predators enable coexistence.Crossref | GoogleScholarGoogle Scholar |

Wodzicki K (1950) ‘Introduced mammals of New Zealand – an ecological and economic survey.’ (Department of Scientific & Industrial Research)