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Protocols in ecological and environmental plant physiology


Crop and Pasture Science is a highly cited and prestigious journal publishing original research on advances in plant sciences, sustainable farming systems, and food quality. More

Editors-in-Chief: Sergio Atienza and Zed Rengel


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Published online 25 July 2016
Genotypic stability of weed competitive ability for bread wheat (Triticum aestivum) genotypes in multiple environments 
M. C. Zerner, G. J. Rebetzke and G. S. Gill

Crop competition is an important component of weed management in modern cropping systems. A large number of wheat genotypes varying in early vigour were evaluated over multiple sites and seasons to identify differences and stability of competitive ability. Several of these experimental high vigour lines showed consistently greater weed suppression than commercial cultivars. A strong association between genotype mean weed suppression and stability across four environments provides evidence for opportunities in the selection of improved weed competitive ability in wheat.

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Published online 14 July 2016
Study of genotype by environment interaction in tall fescue genotypes and their polycross progenies in Iran based on AMMI model analysis 
M. R. Dehghani, M. M. Majidi, A. Mirlohi and G. Saeidi

Information pertaining to GE interaction especially for native genotypes under irrigation conditions is very limited. This study aimed to analyse and understand the complex G × E interactions in three groups of tall fescue genotypes using the AMMI model analysis. The graphical presentation has brought out the complexity in the dataset and has clearly aided in the identification of stable and superior genotypes in some environments.

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Published online 11 July 2016
Tropical dairy pasture yield and nitrogen cycling: effect of urea application rate and a nitrification inhibitor, DMPP 
J. Koci and P. N. Nelson

Tropical dairies use high rates of nitrogen fertiliser and there have been few studies into how fertiliser-use efficiency might be improved. This 1-year study on a dairy farm in tropical North Queensland found that urea application rates could be halved without reducing pasture growth if the urea was coated with a nitrification inhibitor (DMPP). The findings indicate good potential for farmers to maintain productivity and profitability while reducing losses of nitrogen to the environment.

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Published online 07 July 2016
Calibration of GrassMaster II to estimate green and dry matter yield in Mediterranean pastures: effect of pasture moisture content 
João Serrano, Shakib Shahidian and José Marques da Silva

The present study has established a set of GrassMaster II probe calibration equations, whereby it is possible to estimate green matter and dry matter yield for typical Mediterranean pastures based on the corrected meter reading at different phenological stages and pasture moisture contents. The accuracy and the reliability of the probe show that it can be associated with a GPS receiver providing the means for in-situ evaluation and monitoring of pasture development patterns.

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Published online 04 July 2016
Genetic compatibility among morphotypes of Vigna lanceolata and implications for breeding improved cultivars 
R. J. Lawn, Hang T. T. Vu, L. M. Bielig and A. Kilian

Genetic relations between morphotypes of the endemic native legume Vigna lanceolata were studied using a complete diallel cross. It was concluded that genetic incompatibilities will preclude all morphotypes being used in a single breeding program. Two options drawing on subsets of morphotypes are suggested: a tuberous-rooted, amphicarpic perennial for use as a pasture legume, and a freely seeding, fibrous-rooted, amphicarpic annual for use as a ley legume.

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blank image Crop and Pasture Science
Volume 67 Number 6 2016

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Quality potential of synthetic-derived commercial wheat cultivars in south-western China 
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Y. L. Tang , C. S. Li , W. Y. Yang , Y. Q. Wu , X. L. Wu , C. Wu , X. L. Ma , S. Z. Li and G. M. Rosewarne
pp. 583-593

The environmental effect was greater than genotypic for most protein quantity and quality parameters. The genotype effect was greater for falling number and the parameters from the rapid visco analyser. Grain protein content, wet gluten content, falling number, and RVA parameters were all significantly lower in SDCs than NSCs. SDCs exhibit a higher degree of variation in quality parameter, Chuanmai 104 performed well in quality parameters.


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Contribution of apigenin di-C-glycosides and lutein to the colour of yellow alkaline noodles 
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Grace Y. Wijaya , Clare Ingram , Robert E. Asenstorfer and Daryl J. Mares
pp. 594-604

The colour of Asian yellow noodles is important quality that influences consumers. Lutein in wheat flour was reported to contribute to noodle yellowness. However, this is unacceptable for the market because the yellow flour is undesirable in other products such as bread. Contribution of a yellow pigment group in wheat grain and flour that only turns yellow during noodle making was quantified, which also revealed the presence of unidentified compounds.

    | Supplementary Material (643 KB)

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Application of prognostic breeding in maize 
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Vasileios Greveniotis and Vasilia A. Fasoula
pp. 605-620

Prognostic breeding is a crop-improvement methodology that utilizes the plant prognostic equation to maximise selection efficiency, increase the precision of field phenotyping, and select plants with high and stable crop yield potential. Application of prognostic breeding in maize started in the F2 of the hybrid Costanza and was effective in selecting superior lines that averaged 87–91% of Costanza’s productivity. The average annual genetic gain ranged from 23% to 36%.


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Effect of Fe3O4 nanoparticles and iron chelate on the antioxidant capacity and nutritional value of soil-cultivated maize (Zea mays) plants 
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Mahboobeh Jalali , Faezeh Ghanati and Ali Mohammad Modarres-Sanavi
pp. 621-628

The objective of the research was feasibility study of removing iron deficiency-induced chlorosis in maize via Fe 3O4 NPs and comparison its function with Fe-EDDHA. There was no obvious difference between Fe3O4 nanoparticles (NPs) and Fe-EDDHA in vegetative growth parameters of maize but in reproductive growth phase, differences between the treatments were noticeable and showed the advantage of Fe3O4 NPs.


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Competition and growth of a grass–legume mixture fertilised with nitrogen and phosphorus: effect on nutrient acquisition, root morphology and symbiosis with soil microorganisms 
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Rodolfo Mendoza , Ileana García , Daniela Depalma and Carolina Fernández López
pp. 629-640

A nutrient-deficient soil was fertilised with N, P and N + P to pure and mixed stands of Lotus tenuis and Festuca arundinacea to investigate the effects of on plant growth, root morphology, N and P uptake and symbiotic associations with arbuscular mycorrhizae and rhizobia. When P is not limiting, N supply favours competitiveness of the grass. Optimisation of P nutrition might help to maximise N inputs into grasslands by symbiotic N-fixation and decrease inputs of inorganic N.


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Nitrogen fertiliser requirements of high-yielding irrigated transgenic cotton 
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Ian J. Rochester and Michael Bange
pp. 641-648

Nitrogen (N) fertiliser is used to achieve high yields in irrigated cotton systems, but it can be used inefficiently. Cotton experiments conducted over 8 years varied N fertiliser rates and previous cropping history were used to generate yield, crop N uptake, and apparent fertiliser recovery differences. Using initial soil N levels, N uptake and yield better estimates of the economic N fertiliser rate were generated (r2 = 0.80).


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Single independent genes confer resistance to faba bean rust (Uromyces viciae-fabae) in the current Australian cultivar Doza and a central European line Ac1655 
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Kedar N. Adhikari , Peng Zhang , Abdus Sadeque , Sami Hoxha and Richard Trethowan
pp. 649-654

Faba bean rust, caused by Uromyces viciae-fabae, is an important disease of faba beans in the most faba bean growing areas. Our studies showed a wide variation for rust resistance in the Australian faba bean germplasm and a cultivar Doza and a central European line Ac1655 each carried single and independent genes for resistance. Detection of at least two rust resistant genes and potential of finding more provide ample opportunities for improving the rust resistance in faba bean.


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Physiological and molecular characterisation of lucerne (Medicago sativa L.) germplasm with improved seedling freezing tolerance 
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M. Rokebul Anower , Anne Fennell , Arvid Boe , Ivan W. Mott , Michael D. Peel and Yajun Wu
pp. 655-665

Lucerne is a major forage legume grown extensively worldwide with important agronomic and environmental attributes. We report that two lucerne germplasms adapted to the harsh environment of the Grand River National Grasslands (South Dakota, USA) showed greater freezing tolerance and may employ different physiological and molecular mechanisms in stress tolerance. These germplasms can be an important resource in breeding for improving freezing tolerance in lucerne.

    | Supplementary Material (368 KB)

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Autumn dormancy regulates the expression of cas18, vsp and corF genes during cold acclimation of lucerne (Medicago sativa L.) 
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Zhi-ying Liu , Guo-feng Yang , Xi-liang Li , Ya-fei Yan , Juan Sun , Run Gao , Qi-zhong Sun and Zong-li Wang
pp. 666-678

This study mainly examined the influence of four autumn-dormancy categories on cas18, vsp and corF gene transcripts during the first year of lucerne establishment. Results showed that the level of transcripts of cas18, vsp and corF in dormant cultivars were markedly higher than in other autumn-dormancy categories cultivars. These results indicate that autumn dormancy are likely to have significant consequences on lucerne productivity and its long-term persistence.


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Control of water leakage from below the root zone by summer-active pastures is associated with persistence, density and deep rootedness 
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M. R. McCaskill and G. A. Kearney
pp. 679-693

Temperate pastures that leak water below the root zone have been linked to an increase in dryland salinity. We compared soil water beneath eight pasture types in a 5-year grazed field study. Pasture characteristics for control of leakage included summer activity, deep rootedness, and persistence at high plant densities under grazing; only lucerne satisfied all these criteria.


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These articles have been peer reviewed and accepted for publication. They are still in production and have not been edited, so may differ from the final published form.

    CP16185  Accepted 20 July 2016
    Molecular characterisation of novel LMW-m and -s genes from four Aegilops species (Sitopsis section) and comparison with those from the Glu-B3 locus of common wheat
    Susana Cuesta, Carlos Guzman, Juan B. Alvarez

    CP16162  Accepted 19 July 2016
    Response of different fodder legume species to Colletotrichum trifolii
    Irene Jacob, Stephan Hartmann, Christine Struck

    CP16168  Accepted 11 July 2016
    Response of arbuscular mycorrhizal fungi to soil phosphorus patches is context dependent
    Guangzhou Wang, Xia Li, Peter Christie, Junling Zhang, Xiaolin Li

    CP15375  Accepted 26 June 2016
    Effects of ethephon in anatomical changes in sunflowers (Helianthus annuus L) stems associated to lodging
    Mariano Mangieri, Anita Mantese, Alejandro Alvarez Schürmann , Claudio Chimenti

    CP16073  Accepted 23 June 2016
    High-temperature adult-plant (HTAP) stripe rust (Puccinia striiformis f. sp. tritici) resistance in facultative winter wheat
    Beyhn Akın, Xian-Ming Chen, Alexsey Morgounov, Nusret Zencirci, Anmin Wan, Meinan Wang

    CP16159  Accepted 16 June 2016
    Seeding rate and cultivar effects on canola (Brassica napus) competition with volunteer wheat (Triticum aestivum)
    Deirdre Lemerle, David Luckett, Eric Koetz, Trent Potter, Hanwen Wu

    CP16149  Accepted 14 June 2016
    Genotypic evaluation of introduced white clover (Trifolium repens L.) germplasm in New Zealand
    Zulfi Jahufer, John Ford, Derek Woodfield, Brent Barrett

    CP16094  Accepted 12 June 2016
    Light extinction of wheat as affected by N fertilization and plant parameters
    A. Soleymani, Mohammad Miransari

    CP16102  Accepted 03 June 2016
    Low-Cost Automated Biochemical Phenotyping for Optimised Nutrient Quality Components in Ryegrass Breeding
    Luke Pembleton, Junping Wang, German Spangenberg, John Forster, Noel Cogan

    CP16153  Accepted 01 June 2016
    Trends in grain production and yield gaps in the high rainfall zone of southern Australia
    Michael Robertson, John Kirkegaard, Allan Peake, Zoe Creelman, Lindsay Bell, Julianne Lilley, Jon Midwood, Heping Zhang, Susan Kleven, Chris Duff, Roger Lawes, Penny Riffkin

    CP16052  Accepted 30 May 2016
    Vegetative nitrogen stress decreases lodging risk and increases yield of irrigated spring wheat in the subtropics.
    Allan Peake, Kerry Bell, Peter Carberry, Nick Poole, Steven Raine

    CP15250  Accepted 02 May 2016
    Economic and environmental implications of wheat crop sequences on organic dairy farm simulations
    Daniel Abreu, Aaron Hoshide, Ellen Mallory, Erin Roche, André Oliveira, Richard Kersbergen, Rogério Lana, Mozart Fonseca

    CP16091  Accepted 02 May 2016
    Forage and grain yield of common buckwheat in Mediterranean conditions: response to sowing time and irrigation
    Marco Mariotti, Alessandro Masoni, Iduna Arduini

    CP16027  Accepted 14 April 2016
    Interactions between water and nitrogen in Australian cropping systems: physiological, agronomic, economic, breeding and modelling perspectives
    Victor Sadras, P Hayman, Daniel Rodriguez, Marta Monjardino, Martin Bielich, Murray Unkovich, Barry Mudge, Enli Wang

    CP16056  Accepted 29 April 2016
    Recent changes in Southern Australian frost occurrence: implications for wheat production risk
    Steven Crimp, Bangyou Zheng, Nirav Khimashia, David Gobbett, Scott Chapman, Mark Howden, Neville Nicholls

    CP16087  Accepted 24 April 2016
    Expression of amphicarpy in Vigna lanceolata morphotypes and their hybrids and implications for cultivar development
    Bob Lawn, Leone Bielig

    CP15338  Accepted 05 April 2016
    Variation in drought tolerance components and their interrelationships in the core collection of foxtail millet germplasm
    L. Krishnamurthy, Hari Upadhyaya, Junichi Kashiwagi, Ramamoorthy Purushothaman, Sangam Dwivedi, Vincent Vadez

    CP15102  Accepted 04 April 2016
    Diversity for resistance to a moderately virulent bluegreen aphid (Acyrthosiphon kondoi Shinji) population in Trifolium species.
    Alan Humphries, Steve Robinson, David Hawkey, David Peck, Trevor Rowe, Carolyn de Koning, Allen Newman

    CP15226  Accepted 04 April 2016
    Zaid Bello, Sue Walker

    CP15312  Accepted 01 April 2016
    A PCR-based marker closely linked to a 2BS QTL conferring wheat yellow spot resistance for marker assisted breeding
    Fei Ren, Jun Ji, Hui Liu, Martin Barbetti, Kadambot Siddique, Chunji Liu, Guijun Yan

    CP15241  Accepted 29 March 2016
    The effect of water table depth and salinity on growth dynamics of Rhodes grass (Chloris gayana).
    Sebastián Chiacchiera, Nicolás Bertram, Edith Taleisnik, Esteban Jobbágy

    CP15184  Accepted 29 March 2016
    The shift of plant N origins alters C and N assimilation during reproductive stages of soybean grown in a Mollisol
    Yansheng Li, Xiaobing Liu, Guanghua Wang, Zhenhua Yu, Ulrike Mathesius, Judong Liu, Stephen Herbert, Jian Jin

    CP16038  Accepted 25 March 2016
    Berry quality and antioxidant properties in Vitis vinifera L. cv. Tempranillo as affected by clonal variability, mycorrhizal inoculation and temperature
    Nazareth Torres, Nieves Goicoechea, Fermín Morales, María Carmen Antolín

    CP16015  Accepted 21 March 2016
    Maize roots acidify the rhizosphere less, and release less organic acid and acid phosphatase, than faba bean in response to phosphorus deficiency
    Haitao Liu, Philip White, Chunjian Li

    CP14363  Accepted 20 March 2016
    Effects of irrigating forage turnips, Brassica rapa var. rapa cv. Barkant, during different periods of vegetative growth 3. Irrigation water use efficiency, evapotranspiration and effective use of water.
    Barry Rowe, James Neilsen

    CP15421  Accepted 07 March 2016
    Canopy application of film antitranspirants over the reproductive phase enhances yield and yield-related physiological traits of water stressed oilseed rape (Brassica napus L.).
    Michele Faralli, Ivan Grove, Martin Hare, Roger Boyle, Kevin Williams, Fiona Corke, Peter Kettlewell

    CP15331  Accepted 04 March 2016
    Rice cold tolerance across reproductive stages
    Jaquie Mitchell, Siti Zulkafli, Jocelyn Bosse, Bradley Campbell, Peter Snell, Emma Mace, Ian Godwin, Shu Fukai

    CP15260  Accepted 01 March 2016
    Cradle-to-farm gate greenhouse gas emissions for 2-year wheat monoculture and break crop-wheat sequences in south-eastern Australia
    Pip Brock, Sally Muir, David Herridge, Aaron Simmons

    CP15417  Accepted 23 February 2016
    Waterlogging at tillering affects spike and spikelet formation in wheat
    Iduna Arduini, Cecilia Orlandi, Silvia Pampana, Alessandro Masoni

    CP15385  Accepted 23 February 2016
    Cell membrane stability and chlorophyll content variation in wheat (Triticum aestivum L) genotypes under heat and drought conditions
    Shoaib Ur Rehman, Muhammad Bilal, Rashid Rana, Muhammad Tahir, Muhammad Shah, Habtamu Ayalew, Guijun Yan

    CP15311  Accepted 29 January 2016
    Using 33P to trace in situ the fate of canola below-ground phosphorus including wheat uptake in two contrasting soils
    Foyjunnessa Foyjunnessa, Annie McNeill, Ashlea Doolette, Sean Mason, Mike McLaughlin

    CP13382  Accepted 22 November 2013
    Forage Breeding for Changing Environments and Production Systems – an Overview of ISFB 2013
    Kevin Smith, German Spangenberg

    CP13154  Accepted 08 June 2013
    Effect of Irrigation Regimes on Spring Seeding of Canola Cultivars under Semi-Arid and High Elevation Conditions
    Jamshid Razmjoo, Seyed Eatesam Ghaemmaghami

    CP13156  Accepted 14 May 2013
    The career and contribution to Australian and International Agricultural Science of Clive McDonald Francis: An introduction
    Mike Ewing, David Chatel, Michael Poole, William Collins


The Most Read ranking is based on the number of downloads from the CSIRO PUBLISHING website of articles published in the previous 12 months. Usage statistics are updated daily.

Rank Paper Details
1. Published 30 September 2015
Morphological, physiological and yield responses of durum wheat to pre-anthesis water-deficit stress are genotype-dependent

Haipei Liu, Iain R. Searle, Diane E. Mather, Amanda J. Able and Jason A. Able

2. Published 29 October 2015
Adaptation of wheat, barley, canola, field pea and chickpea to the thermal environments of Australia

Victor Sadras and M. Fernanda Dreccer

3. Published 31 July 2015
Can wheat varietal mixtures buffer the impacts of water deficit?

Paul Adu-Gyamfi, Tariq Mahmood and Richard Trethowan

4. Published 4 September 2015
Assessing the place and role of crop simulation modelling in Australia

M. J. Robertson, G. J. Rebetzke and R. M. Norton

5. Published 29 February 2016
Frost-tolerance genes Fr-A2 and Fr-B2 in Australian wheat and their effects on days to heading and grain yield in lower rainfall environments in southern Australia

H. A. Eagles, Jayne Wilson, Karen Cane, Neil Vallance, R. F. Eastwood, Haydn Kuchel, P. J. Martin and Ben Trevaskis

6. Published 29 January 2016
Soil compaction and controlled traffic considerations in Australian cotton-farming systems

Diogenes L. Antille, John McL. Bennett and Troy A. Jensen

7. Published 4 September 2015
Statistical methods for analysis of multi-harvest data from perennial pasture variety selection trials

Joanne De Faveri, Arūnas P. Verbyla, Wayne S. Pitchford, Shoba Venkatanagappa and Brian R. Cullis

8. Published 30 September 2015
Saline water irrigation of quinoa (Chenopodium quinoa) under Mediterranean conditions

Attila Yazar, Çigdem Incekaya, S. Metin Sezen and Sven-Erik Jacobsen

9. Published 31 May 2016
The history of using rainfall data to improve production in the grain industry in Australia—from Goyder to ENSO

Derek Yates, R. Willem Vervoort, Budiman Minasny and Alex McBratney

10. Published 6 May 2016
Nitrogen management to optimise canola production in Australia

R. M. Norton

11. Published 29 October 2015
Sequential zinc and iron biofortification of bread-wheat grains: from controlled to uncontrolled environments

Fernando C. Lidon, Ana S. Almeida, Ana R. Costa, Ana S. Bagulho, Paula Scotti-Campos, José N. Semedo, Benvindo Maçãs, José Coutinho, Nuno Pinheiro, Conceição Gomes, António E. Leitão, Isabel P. Pais, Maria M. Silva, Fernando H. Reboredo, Maria F. Pessoa and José C. Ramalho

12. Published 29 January 2016
Nitrogen uptake efficiency of maize in monoculture and intercropped with Brachiaria humidicola and Panicum maximum in a dystrophic Red-Yellow Latosol of the Brazilian Cerrado

Thais Rodrigues Coser, Maria Lucrécia Gerosa Ramos, Cícero Célio de Figueiredo, Segundo Urquiaga, Arminda Moreira de Carvalho, Filipe Vieira Barros and Maria Thereza Mendonça

13. Published 30 September 2015
Impacts of endophyte infection of ryegrass on rhizosphere metabolome and microbial community

S. Wakelin, S. Harrison, C. Mander, B. Dignam, S. Rasmussen, S. Monk, K. Fraser and M. O'Callaghan

14. Published 29 February 2016
Timing and rates of nitrogen fertiliser application on seed yield, quality and nitrogen-use efficiency of canola

B. L. Ma and A. W. Herath

15. Published 29 October 2015
In search of photosensitising compounds in the annual forage legume Biserrula pelecinus L.

E. Swinny, C. K. Revell, N. Campbell, E. Spadek and C. Russo

16. Published 29 October 2015
Effect of the maize–soybean intercropping system on the potential bioavailability of magnesium, iron and zinc

Vesna Dragicevic, Snezana Oljaca, Milovan Stojiljkovic, Milena Simic, Zeljko Dolijanovic and Natalija Kravic

17. Published 21 December 2015
Role of magnesium in food production: an overview

A. R. Dechen, Q. A. C. Carmello, F. A. Monteiro and R. C. Nogueirol

18. Published 29 February 2016
Patterns of water stress and temperature for Australian chickpea production

Lachlan Lake, Karine Chenu and Victor O. Sadras

19. Published 29 January 2016
Pasture mixes with lucerne (Medicago sativa) increase yields and water-use efficiencies over traditional pastures based on subterranean clover (Trifolium subterraneum)

M. R. McCaskill, M. C. Raeside, S. G. Clark, C. MacDonald, B. Clark and D. L. Partington

20. Published 6 May 2016
Simulation of growth, development and yield of canola (Brassica napus) in APSIM

M. J. Robertson and J. M. Lilley

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