Changes in feed intake during isolation stress in respiration chambers may impact methane emissions assessmentPol Llonch A C D , Shane M. Troy B , Carol-Anne Duthie B , Miguel Somarriba A , John Rooke B , Marie J. Haskell A , Rainer Roehe A and Simon P. Turner A
A Animal and Veterinary Sciences Group, SRUC (Scotland’s Rural College), West Mains Road, Edinburgh, EH9 3JG, UK.
B Future Farming Systems Group, SRUC (Scotland’s Rural College), West Mains Road, Edinburgh, EH9 3JG, UK.
C Present address: School of Veterinary Science, Universitat Autònoma de Barcelona, 08193 Cerdanyola del Vallès, Barcelona, Spain.
D Corresponding author. Email: firstname.lastname@example.org
Animal Production Science - https://doi.org/10.1071/AN15563
Submitted: 11 September 2015 Accepted: 12 December 2015 Published online: 25 February 2016
Respiration chambers are considered the ‘gold standard’ technique for measuring in vivo methane (CH4) emissions in live animals. However, the imposed isolation required may alter feeding behaviour and intake, which ultimately impact CH4 emissions. The aim of this study was to assess the impact of isolation within respiration chambers on feed intake and CH4 emissions with two different diets and breeds of beef cattle. In addition, a routine stressor (transport) was used to examine the relationship between individual stress responsiveness and changes in feed intake during isolation. Eighty-four steers (castrated males) (569 ± 5.7 kg bodyweight, BW) were divided into two groups and each group fed with one of two basal diets consisting of (g/kg dry matter, DM) either 50 : 50 (Mixed) or 8 : 92 (Concentrate) forage to concentrate ratios. Within each basal diet there were three supplementation treatments: (1) control, (2) calcium nitrate, and (3) rapeseed cake. The stress biomarkers plasma cortisol, creatine kinase (CK), and free fatty acids (FFA) were determined before (0 h) and after (30 min, 3 h, 6 h and 9 h) a 30-min journey, when steers were transported to the respiration chamber facilities. Methane emissions were measured over a 3-day period using individual respiration chambers. Dry matter intake (DMI) was assessed within the group-housed pens (4 weeks before entry to training pen), in the training pens and the chambers. Cortisol, FFA and CK increased (P < 0.05) after transport confirming a stress response. DMI (g/kg BW) decreased (P < 0.001) during isolation in the training pens (14.7 ± 0.28) and the chambers (14.3 ± 0.26) compared with that of the same animals in the group pens (16.8 ± 0.23). DMI during isolation decreased more in those animals which had an increased (P < 0.05) stress response during transport as measured by cortisol, FFA and CK. With the Mixed diet, the decline in DMI was estimated to result in an increase in CH4 (g/kg DMI) (r = 0.25, P = 0.001), which did not occur with the Concentrate diet. According to the results of this experiment, the stress associated with isolation reduces the DMI resulting in an increase in g CH4/kg DMI in fibrous diets. Habituation to isolation needs refinement in order to reduce the impact of stress on intake and therefore achieve more accurate estimates of CH4 emissions. Alternatively, modelling CH4 estimations according to behavioural and physiological changes associated with isolation stress would improve accuracy of CH4 estimations.
Additional keywords: beef cattle, feeding behaviour, stress physiology.
ReferencesAl-Dujaili EA, Baghdadi HH, Howie F, Mason JI (2012) Validation and application of a highly specific and sensitive ELISA for the estimation of cortisone in saliva, urine and in vitro cell-culture media by using a novel antibody. Steroids 77, 703–709.
| Validation and application of a highly specific and sensitive ELISA for the estimation of cortisone in saliva, urine and in vitro cell-culture media by using a novel antibody.CrossRef | 1:CAS:528:DC%2BC38XkvFSrsbc%3D&md5=0d8bbf6057b766747937b41fa369d1d4CAS | 22429925PubMed |
Albright JL (1993) Feeding behavior of dairy cattle. Journal of Dairy Science 76, 485–498.
| Feeding behavior of dairy cattle.CrossRef |
Boissy A, Le Neindre P (1997) Behavioral, cardiac and cortisol responses to brief peer separation and reunion in cattle. Physiology & Behavior 61, 693–699.
| Behavioral, cardiac and cortisol responses to brief peer separation and reunion in cattle.CrossRef | 1:CAS:528:DyaK2sXjtVGns78%3D&md5=4334cb86b532d77c4579d8ffda7ce93dCAS |
Buddle BM, Denis M, Attwood GT, Altermann E, Janssen PH, Ronimus RS, Pinares-Patiño CS, Muetzel S, Wedlock DN (2011) Strategies to reduce methane emissions from farmed ruminants grazing on pasture. Veterinary Journal (London, England) 188, 11–17.
| Strategies to reduce methane emissions from farmed ruminants grazing on pasture.CrossRef | 1:CAS:528:DC%2BC3MXjs1Sgt7k%3D&md5=442f7d210a5d792ca27315cd1168f6c3CAS |
Duthie CA, Rooke JA, Troy S, Hyslop JJ, Ross DW, Waterhouse A, Roehe R (2015) Impact of adding nitrate or increasing the lipid content of two contrasting diets on blood methaemoglobin and performance of two breeds of finishing beef steers. Animal
| Impact of adding nitrate or increasing the lipid content of two contrasting diets on blood methaemoglobin and performance of two breeds of finishing beef steers.CrossRef | 26627142PubMed | in press.
Fisher AD, Crowe MA, Alonso De La Varga ME, Enright WJ (1996) Effect of castration method and the provision of local anesthesia on plasma cortisol, scrotal circumference, growth, and feed intake of bull calves. Journal of Animal Science 74, 2336–2343.
Galyean ML, Hubbert ME (1995) Effects of season, health, and management on feed intake by beef cattle. In ‘Symposium: intake by feedlot cattle’. (Ed. FN Owens) pp. 226–234. (Oklahoma Agricultural Experiment Station)
Gerber PJ, Hristov AN, Henderson B, Makkar H, Oh J, Lee C, Meinena R, Montesa F, Otta T, Firkinsa J, Rotza A, Della C, Adesogana AT, Yanga WZ, Tricaricoa JM, Kebreaba E, Waghorna G, Dijkstraa J, Oosting S (2013) Technical options for the mitigation of direct methane and nitrous oxide emissions from livestock: a review. Animal 7, 220–234.
| Technical options for the mitigation of direct methane and nitrous oxide emissions from livestock: a review.CrossRef | 23739465PubMed |
Grainger C, Clarke T, McGinn SM, Auldist MJ, Beauchemin KA, Hannah MC, Waghorn GC, Clark H, Eckard RJ (2007) Methane emissions from dairy cows measured using the sulfur hexafluoride (SF 6) tracer and chamber techniques. Journal of Dairy Science 90, 2755–2766.
| Methane emissions from dairy cows measured using the sulfur hexafluoride (SF 6) tracer and chamber techniques.CrossRef | 1:CAS:528:DC%2BD2sXlvFOitr0%3D&md5=9edcc5a363c2eba212f8765f0901b599CAS | 17517715PubMed |
Grandin T (1997) Assessment of stress during handling and transport. Journal of Animal Science 75, 249–257.
Harbuz MS, Lightman SL (1992) Stress and the hypothalamo-pituitary-adrenal axis: acute, chronic and immunological activation. The Journal of Endocrinology 134, 327–339.
| Stress and the hypothalamo-pituitary-adrenal axis: acute, chronic and immunological activation.CrossRef | 1:CAS:528:DyaK38XlsVelsr8%3D&md5=d88247052e28b6269b2b52fb933676eaCAS | 1402543PubMed |
Hutcheson DP, Cole NA (1986) Management of transit-stress syndrome in cattle: nutritional and environmental effects. Journal of Animal Science 62, 555–560.
International Panel of Climate Change (IPCC) (2013) Climate Change 2014: Mitigation of Climate Change. Working Group III 5th Assessment Report. Chapter 11 – Agriculture, Forestry and Other Land Use. (Cambridge University Press: New York, NY)
Matter RL, Carroll JA, Dyer CJ (2000) Neuroendocrine responses to stress. In ‘The biology of animal stress: basic principles and implications for animal welfare’. (Eds GP Moberg, JA Mench) Chapter 3, pp. 43–70. (CABI Publishing: Wallingford, UK)
Mialon MM, Deiss V, Andanson S, Anglard F, Doreau M, Veissier I (2012) An assessment of the impact of rumenocentesis on pain and stress in cattle and the effect of local anaesthesia. Veterinary Journal (London, England) 194, 55–59.
| An assessment of the impact of rumenocentesis on pain and stress in cattle and the effect of local anaesthesia.CrossRef |
Palme R, Robia C, Baumgartner W, Möstl E (2000) Transport stress in cattle as reflected by an increase in faecal cortisol metabolite concentrations. The Veterinary Record 146, 108–109.
| Transport stress in cattle as reflected by an increase in faecal cortisol metabolite concentrations.CrossRef | 1:STN:280:DC%2BD3c7ksV2jsA%3D%3D&md5=654a3da6906f179296fa37e2767ebd38CAS | 10682697PubMed |
Ricci P, Chagunda MGG, Rooke J, Houdijk JGM, Duthie CA, Hyslop J, Roehe R, Waterhouse A (2014) Evaluation of the laser methane detector to estimate methane emissions from ewes and steers. Journal of Animal Science 92, 5239–5250.
| Evaluation of the laser methane detector to estimate methane emissions from ewes and steers.CrossRef | 1:CAS:528:DC%2BC2MXitlalsA%3D%3D&md5=f01f2684e438dd3aee6ee649bbaf74aeCAS | 25349366PubMed |
Sapolsky RM (2002) Endocrinology of the stress-response. In ‘Behavioral endocrinology’. (Eds JB Becker, SM Breedlove, D Crews, MM MacCarthy) p. 409–450.
Troy SM, Duthie CA, Hyslop JJ, Roehe R, Ross DW, Wallace RJ, Waterhouse A, Rooke JA (2015) Effectiveness of nitrate addition and increased oil content as methane mitigation strategies for beef cattle fed two contrasting basal diets. Journal of Animal Science 93, 1815–1823.
| Effectiveness of nitrate addition and increased oil content as methane mitigation strategies for beef cattle fed two contrasting basal diets.CrossRef | 1:CAS:528:DC%2BC2MXoslWrt7s%3D&md5=29e6f671af83d2f713f69c5506b31746CAS | 26020202PubMed |