Effects of an antler velvet-based natural compound on osteoporosis in a rodent model
Haiping Zhao
A , Xuehua Wang A B , Zhen Wang A , Fangzhou Du A , Haitao Li B D and Chunyi Li A C D
+ Author Affiliations
- Author Affiliations
A Institute of Special Wild Economic Animals and Plants, Chinese Academy of Agricultural Sciences, No. 4899, Juye Street, Changchun, China.
B College of Pharmacy, Nanjing University of Chinese Medicine, No. 138, Xianlin Street, Nanjing 210023, China.
C Changchun Sci-Tech University, No. 1699, Donghua Street, Changchun 130600, China.
D Corresponding author. Email: lichunyi1959@163.com
Animal Production Science 60(10) 1334-1342 https://doi.org/10.1071/AN19682
Submitted: 6 September 2019 Accepted: 7 December 2019 Published: 20 April 2020
Abstract
Context: Velvet antlers (VA) are claimed to have the effects on osteoporosis in traditional Chinese medicine.
Aims: So as to scientifically confirm this claim, a VA-based compound (VAC, the mixture of upper part of VA, deer blood, and calcined oyster shell powder in a ratio of 4 : 1 : 1) was produced and administered to osteoporotic model rats, with osteoporosis being induced by retinoic acid via gavage.
Methods: In total, 48 rats were used and divided into six groups (8/group). Concentrations of alkaline phosphatase and osteocalcin in the rat serum were measured, and bone ash weight, concentrations of calcium and phosphorus in rat femur were measured. Bone biomechanical test was performed using a computer-controlled three-point bending tester. Ratio of trabecular bone area to tissue area in a given view field of the femoral tissue section was histologically examined and calculated. Bone histomorphometry was measured using micro-CT.
Key results: No significant difference was found between the VAC-treated groups and the positive control (alendronate sodium) on the basis of the following tested parameters: (1) levels of alkaline phosphatase and osteocalcin in rat serum; (2) maximum load value (N) of femur and maximum compression force of lumbar vertebra; (3) concentrations of calcium and phosphorus in femur; (4) ratio of trabecular bone area to tissue area; and (5) bone histomorphometry.
Conclusions: Effects of VAC used in the present study on osteoporosis in the model rats were comparable to the alendronate sodium (western medicine for treating osteoporosis) on the basis of our selected parameters.
Implications: This compound has the potential to be developed as an effective traditional Chinese medicine for clinic use to treat osteoporosis.
Additional keywords: calcined oyster shell, deer blood, osteoporosis, velvet antler.
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