Australian Journal of Chemistry Australian Journal of Chemistry Society
An international journal for chemical science

Aptamer-Based Fluorescent Switch for Sensitive Detection of Oxytetracycline

Mahsa Babaei A H , Seyed Hamid Jalalian B C H , Hadi Bakhtiari C , Mohammad Ramezani D , Khalil Abnous A E I and Seyed Mohammad Taghdisi F G I
+ Author Affiliations
- Author Affiliations

A Pharmaceutical Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

B Department of Pharmaceutical Nanotechnology, Students Research Committee, Mashhad University of Medical Sciences, Mashhad, Iran.

C Academic Center for Education, Culture and Research (ACECR)-Mashhad Branch, Mashhad, Iran.

D Nanotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

E Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.

F Targeted Drug Delivery Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.

G Department of Pharmaceutical Biotechnology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran.

H These authors contributed equally to the work.

I Corresponding authors. Email:;

Australian Journal of Chemistry 70(6) 718-723
Submitted: 3 October 2016  Accepted: 24 October 2016   Published: 16 November 2016


Oxytetracycline (OTC) is one of the most used antibiotics in veterinary medicine. There is a large concern about developing antibiotic resistance in humans as a result of the consumption of products contaminated with OTC, so a fast detection technique for an on-field screening test is highly in demand. Here we introduce a novel aptasensor for fast detection of OTC, based on a triple helix molecular switch (THMS) complex formation. The limit of detection (LOD) of this sensor was 1.67 and 6.44 nM in phosphate buffer and milk samples, respectively. Moreover, the sensor showed a high selectivity towards OTC.


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