Australian Journal of Chemistry Australian Journal of Chemistry Society
An international journal for chemical science

Influence of Adsorbent Nature on the Dynamic Headspace Study of Insect Semiochemicals

Sergio A. Rodriguez A B , María L. Paliza A and Monica A. Nazareno A
+ Author Affiliations
- Author Affiliations

A Centro de Investigaciones y Transferencia Santiago del Estero (CITSE)-CONICET-Universidad Nacional de Santiago del Estero (UNSE), Santiago del Estero 4200, Argentina.

B Corresponding author. Email:

Australian Journal of Chemistry 70(8) 902-907
Submitted: 2 February 2017  Accepted: 14 March 2017   Published: 3 April 2017


In chemical ecology studies (insect–insect, insect–plant relationships), it is important to choose the appropriate sampling methods and the correct optimization of sampling by using dynamic systems. In the present work, different adsorbents were evaluated in a dynamic system that presents a stream of purified air flowing through an aeration chamber containing a mixture of volatile organic compounds, mainly insect semiochemicals such as α-pinene, sulcatone, β-linalool, menthone, isomenthone, methyl salicylate, grandlure I, grandlure II, grandlure III, grandlure IV, eugenol, and α-ionone. Traditional adsorbents such as Tenax TA, Porapak Q, Hayesep Q, and activated charcoal were evaluated; further, alternatives such as Porapak Rxn RP, HLB, SCX, and silica gel, among others were proposed owing to their lower cost. The results demonstrated that Porapak Q and Porapak Rxn RP, despite their different chemical composition, were able to produce similar ratios of compounds to that of the reference solution and, moreover, with the highest recovery yields. However, it is important to emphasize the adsorption selectivity provided by SCX for eugenol and α-ionone. When Porapak Rxn RP was used in the analysis of Eucalyptus globulus volatiles, excellent results were obtained, and these agree with reported data from a hydrodistillation method.


[1]  C. Malosse, P. Ramirez-Lucas, D. Rochat, J. P. Morin, J. High Resolut. Chromatogr. 1995, 18, 669.
         | CrossRef | 1:CAS:528:DyaK2MXhtVSmu7zP&md5=def4179fbc323b6fd263e4214b15af74CAS | open url image1

[2]     (a) H. E. Hummel, T. A. Miller, Techniques in Pheromone Research 1984 (Springer-Verlag Inc.: New York, NY).
         (b) J. G. Millar, J. J. Sims, in Methods in Chemical Ecology (Eds J. G. Millar, K. E. Haynes) 1998, pp. 1–31 (Kluwer-Academic Publishers: Norwell, MA).
      (c) P. H. G. Zarbin, J. T. B. Ferreira, W. S. Leal, Quim. Nova 1999, 22, 263.
         | CrossRef | open url image1

[3]  (a) A. C. Soria, M. J. García-Sarrió, M. L. Sanz, Trends Analyt. Chem. 2015, 71, 85.
         | CrossRef | 1:CAS:528:DC%2BC2MXhtVOls7rE&md5=d06c4e63234d974c495f7831efdf5e72CAS | open url image1
         (b) B. Kolb, L. S. Ettre, Static Headspace Gas Chromatography: Theory and Practice 1997 (Wiley–VCH: New York, NY).

[4]  C. F. Ross, in Comprehensive Sampling and Sample Preparation (Ed. J. Pawliszyn) 2012, pp. 27–50 (Elsevier: Amsterdam).

[5]  Z. Zhang, J. Pawliszyn, Anal. Chem. 1995, 67, 34.
         | CrossRef | 1:CAS:528:DyaK2MXis1ClsL8%3D&md5=9a9848524841a4f77230391c5f047e1aCAS | open url image1

[6]  J. Fäldt, M. Eriksson, I. Valterovác, A. K. Borg-Karlson, Z. Naturforsch C 2000, 55c, 180. open url image1

[7]  S. A. Rodríguez, M. L. P. Pérez, M. A. Nazareno, Bull. Entomol. Res. 2016, 106, 494.
         | CrossRef | open url image1

[8]  (a) D. W. Ross, G. E. Daterman, A. S. Munson, West. N. Am. Naturalist 2005, 65, 123. open url image1
      (b) D. S. Pureswaran, R. Gries, J. H. Borden, Chemoecology 2004, 14, 59.
         | CrossRef | open url image1
      (c) D. P. W. Huber, R. Gries, J. H. Borden, H. D. Pierce, Chemoecology 2000, 10, 103.
         | CrossRef | open url image1
      (d) A. Schierling, K. Seifert, S. R. Sinterhauf, J. B. Rieß, J. C. Rupprecht, K. Dettner, Chemoecology 2013, 23, 45.
         | CrossRef | open url image1

[9]  P. Gonzalez-Audino, R. Griffo, P. Gatti, G. Allegro, E. Zerba, Agrofor. Syst. 2013, 87, 109.
         | CrossRef | open url image1

[10]  (a) A. Giglio, P. Brandmayr, R. Dalpozzo, G. Sindona, A. Tagarelli, F. Talarico, T. Brandmayr, E. A. Ferrero, Microsc. Res. Tech. 2009, 72, 351.
         | CrossRef | 1:CAS:528:DC%2BD1MXmsVequ78%3D&md5=9ac8a75ce081a3d6c7180fa9491164e9CAS | open url image1
      (b) B. Y. Han, Z. M. Chen, J. Agric. Food Chem. 2002, 50, 2571.
         | CrossRef | open url image1
      (c) C. A. MacKay, J. D. Sweeney, N. K. Hillier, J. Insect Physiol. 2015, 83, 65.
         | CrossRef | open url image1

[11]  R. Ramachandran, Z. R. Khan, P. Caballero, B. O. Juliano, J. Chem. Ecol. 1990, 16, 2647.
         | CrossRef | 1:CAS:528:DyaK3MXjvFGktA%3D%3D&md5=7acc344b5a1ad98700aec7d04839f3f3CAS | open url image1

[12]  J. D. Warthen, C. Lee, E. B. Jang, D. R. Lance, D. O. McInnis, J. Chem. Ecol. 1997, 23, 1891.
         | CrossRef | 1:CAS:528:DyaK2sXlt1Cgu7k%3D&md5=d425237e66aeb66e4e830b43f1533c1cCAS | open url image1

[13]  (a) F. R. N. Knoll, L. M. Santos, Rev. Bras. Entomol. 2012, 56, 481.
         | CrossRef | open url image1
      (b) T. Shimoda, Exp. Appl. Acarol. 2010, 50, 9.
         | CrossRef | open url image1

[14]  D. Szczerbowski, G. Torrens, M. Rodrigues, O. Trevisan, S. Gomes, A. Tröger, K. Mori, W. Francke, P. Zarbin, Tetrahedron Lett. 2016, 57, 2842.and references therein.
         | CrossRef | 1:CAS:528:DC%2BC28XptVSqur4%3D&md5=9c240d7eb0b15dac752b630813f6e133CAS | open url image1

[15]  (a) Z. Szendrei, A. Averill, H. Alborn, C. Rodriguez-Saona, J. Chem. Ecol. 2011, 37, 387.
         | CrossRef | 1:CAS:528:DC%2BC3MXnvFCisL0%3D&md5=4c2e3ad603e30f3555bba812f0ffa6adCAS | open url image1
      (b) F. J. Eller, R. J. Bartelt, B. S. Shasha, D. J. Schuster, D. G. Riley, P. A. Stansly, T. F. Mueller, K. D. Shuler, B. Johnson, J. H. Davis, C. A. Sutherland, J. Chem. Ecol. 1994, 20, 1537.
         | CrossRef | open url image1
      (c) J. C. Dickens, G. D. Prestwich, J. Chem. Ecol. 1989, 15, 529.
         | CrossRef | open url image1

[16]  J. A. Byers, G. Birgersson, W. Francke, Chemoecology 2013, 23, 251.
         | CrossRef | 1:CAS:528:DC%2BC3sXhslOksb%2FI&md5=7b25e561cebde8ce2146434069f5a51eCAS | open url image1

[17]  (a) K. H. Tan, R. Nishida, J. Insect Sci. 2012, 12, 1.
         | CrossRef | open url image1
      (b) R. Chen, M. Klein, C. Sheng, Y. Lia, Q. Lid, J. Asia Pac. Entomol. 2013, 16, 479.
         | CrossRef | open url image1

[18]  (a) E. A. Lingenfelter, R. N. Williams, L. W. Haynes, D. S. Fickle, Entomol. Sci. 2003, 38, 104. open url image1
      (b) R. N. Williams, D. S. Fickle, T. P. McGovern, M. G. Klein, J. Econ. Entomol. 2000, 93, 1480.
         | CrossRef | open url image1

[19]  N. G. Agelopoulos, J. H. Pickett, J. Chem. Ecol. 1998, 24, 1161.
         | CrossRef | 1:CAS:528:DyaK1cXksVOqsrc%3D&md5=596ff771abf17148dce3456727408edfCAS | open url image1

[20]  (a) M. Schneider, K. U. Goss, Anal. Chem. 2009, 81, 3017.
         | CrossRef | 1:CAS:528:DC%2BD1MXjsFyksb8%3D&md5=f6b3228d0fb51a94976ccf846fe706e4CAS | open url image1
      (b) S. K. Poole, C. F. Poole, Anal. Commun. 1996, 33, 353.
         | CrossRef | open url image1
      (c) M. H. Abraham, D. P. Walsh, J. Chromatogr. A 1992, 627, 294.
         | CrossRef | open url image1

[21]  A. A. Il’ina, A. Yu. Ryabov, A. V. Chuikin, A. A. Velikov, J. Anal. Chem. 2015, 70, 125.
         | CrossRef | 1:CAS:528:DC%2BC2MXhsVClsL4%3D&md5=baf1aa0098f7aaa5a17b03980bcb80d7CAS | open url image1

[22]  H. Singh, S. Kaur, K. Negi, S. Kumari, V. Saini, D. Batish, R. Kohli, LWT – Food Sci. Tech. 2012, 48, 237.
         | CrossRef | 1:CAS:528:DC%2BC38Xntlaltr8%3D&md5=428ac0176e1adacf744000f045745053CAS | open url image1

[23]  H. Marzoug, M. Romdhane, A. Lebrihi, F. Mathieu, F. Couderc, M. Abderraba, M. Khouja, J. Bouajila, Molecules 2011, 16, 1695.
         | CrossRef | open url image1

[24]  A. Elaissi, Z. Rouis, N. Salem, S. Mabrouk, Y. Salem, K. Salah, M. Aouni, F. Farhat, R. Chemli, F. Harzallah-Skhiri, M. Khouja, BMC Complement. Altern. Med. 2012, 12, 81.
         | CrossRef | 1:CAS:528:DC%2BC38Xhslehs73M&md5=ab3d1ff72d4125c12d07e4bb3600d26bCAS | open url image1

[25]  H. Fadel, F. Marx, A. El-Sawy, A. El-Ghorab, Z. Lebensm. Unters. Forsch. A. 1999, 208, 212.
         | CrossRef | 1:CAS:528:DyaK1MXjvVCksr8%3D&md5=eafccdb723f2e5a709080f896740f3f9CAS | open url image1

[26]  F. Wang, J. Deng, C. Schal, Y. Lou, G. Zhou, B. Ye, X. Yin, Z. Xu, L. Shen, Sci. Rep. 2016, 6, 32666.
         | CrossRef | 1:CAS:528:DC%2BC28XhsVOqsr%2FM&md5=bd43205a8843446faf69d05ee6b48da9CAS | open url image1

[27]  A. Lucia, L. Juan, E. Zerba, L. Harrand, M. Marcó, H. Masuh, Parasitol. Res. 2012, 110, 1675.
         | CrossRef | open url image1

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