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Changes in DNA methylation are associated with heterogeneous cytoplasm suppression of the multi-ovary gene in wheat (Triticum aestivum L.)
DUOII is a multi-ovary wheat variety that has 2–3 pistils and 3 stamens. The multi-ovary trait is controlled by a dominant gene, the expression of which can be suppressed by the special heterogeneous cytoplasm of TeZhiI (TZI). TZI has the nucleus of common wheat and the cytoplasm of Aegilops. DUOII (♀) × TZI (♂) shows multi-ovary trait, while TZI (♀) × DUOII (♂) shows mono-ovary. DNA methylation affects gene expression and plays a crucial role in organ and tissue differentiation. To study the relationship between DNA methylation and the suppression of multi-ovary gene, we used methylation sensitive amplification polymorphisms (MSAP) to assess the DNA methylation status of the reciprocal crosses. Genome-wide, 14,584 CCGG sites were detected and the overall methylation levels were 31.10% and 30.76% in the crosses DUOII × TZI and TZI × DUOII, respectively. Meanwhile, compared to DUOII × TZI, there were 672 sites (4.61%) in which the methylation/demethylation processes occurred in TZI × DUOII. These results showed that the special heterogeneous cytoplasm significantly changed DNA methylation, which might have suppressed the multi-ovary gene. These results provide general insight into the patterns of DNA methylation changing in the suppression of the multi-ovary gene, and provide essential background for further studies on the underlying mechanisms of heterogeneous cytoplasm suppression of the expression of the multi-ovary gene in wheat.
CP17410 Accepted 09 January 2018
© CSIRO 2018