Crop and Pasture Science Crop and Pasture Science Society
Plant sciences, sustainable farming systems and food quality

Just Accepted

This article has been peer reviewed and accepted for publication. It is in production and has not been edited, so may differ from the final published form.

Changes in DNA methylation are associated with heterogeneous cytoplasm suppression of the multi-ovary gene in wheat (Triticum aestivum L.)

Jialin Guo , gaisheng zhang , Huali Tang , Yulong Song , Shoucai Ma , Na Niu , Junwei Wang


DUOII is a multi-ovary wheat variety that has 2–3 pistils and 3 stamens. The multi-ovary trait is controlled by a dominant gene, the expression of which can be suppressed by the special heterogeneous cytoplasm of TeZhiI (TZI). TZI has the nucleus of common wheat and the cytoplasm of Aegilops. DUOII (♀) × TZI (♂) shows multi-ovary trait, while TZI (♀) × DUOII (♂) shows mono-ovary. DNA methylation affects gene expression and plays a crucial role in organ and tissue differentiation. To study the relationship between DNA methylation and the suppression of multi-ovary gene, we used methylation sensitive amplification polymorphisms (MSAP) to assess the DNA methylation status of the reciprocal crosses. Genome-wide, 14,584 CCGG sites were detected and the overall methylation levels were 31.10% and 30.76% in the crosses DUOII × TZI and TZI × DUOII, respectively. Meanwhile, compared to DUOII × TZI, there were 672 sites (4.61%) in which the methylation/demethylation processes occurred in TZI × DUOII. These results showed that the special heterogeneous cytoplasm significantly changed DNA methylation, which might have suppressed the multi-ovary gene. These results provide general insight into the patterns of DNA methylation changing in the suppression of the multi-ovary gene, and provide essential background for further studies on the underlying mechanisms of heterogeneous cytoplasm suppression of the expression of the multi-ovary gene in wheat.

CP17410  Accepted 09 January 2018

© CSIRO 2018