This article has been peer reviewed and accepted for publication. It is in production and has not been edited, so may differ from the final published form.
Analysis of phenotype characterization and expression pattern of genes related mainly to carbohydrate metabolism and sporopollenin in male-sterile anthers induced by high temperature in wheat (Triticum aestivum L.)
Male reproductive development in higher plants is highly sensitive to various stressors, including high temperature (HT). In this study, physiologic male-sterile plants of wheat (Triticum aestivum L.) were established using HT induction. The physiologic changes and expression levels of genes mainly related to carbohydrate metabolism and sporopollenin in male-sterile processes were studied using biological techniques, including KI-I2 staining, paraffin sectioning, scanning electron microscopy (SEM) and fluorescent quantitative analysis. The paraffin sectioning and SEM results revealed that parts of HT male-sterile anthers, including the epidermis and tapetum, were remarkably different from those of normal anthers. The expression levels of TaSUT1, TaSUT2, IVR1, and IVR5 were significantly lower than those of normal anthers at early the microspore stage and the trinuclear stage. The RAFTIN1 gene and the TaMS26 gene may contribute to massive sporopollenin to pollen intine; however, their expression levels were significantly higher at the early tetrad stage (ETs) and early microspore stage (Ems) in HT sterile anthers. The recently cloned MS1 gene was expressed at the ETs and EMs but not at the trinuclear stage. Moreover, this gene showed extremely significant high expression in HT sterile anthers compared with normal anthers. These results demonstrate that the HT induction of wheat male sterility is probably related to the expression of genes related to carbohydrate metabolism and sporopollenin metabolism. This provides a theoretical basis and technological approach for further studies on the mechanisms of HT induction of male sterility.
CP18034 Accepted 22 February 2018
© CSIRO 2018