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RESEARCH ARTICLE
Contents Vol 61(3)

Is cotton-strip tensile strength a surrogate for microbial activity in groundwater?

M. J. Lategan A B D F , K. Korbel B E and G. C. Hose C D E
+ Author Affiliations
- Author Affiliations

A Centre for Environmental Sustainability, University of Technology, PO Box 123, Broadway, Sydney, NSW 2007, Australia.

B Department of Environmental Sciences, University of Technology, PO Box 123, Broadway, Sydney, NSW 2007, Australia.

C Departments of Biological Sciences & Environment and Geography, Macquarie University, Sydney, NSW 2109, Australia.

D CRC for Contamination Assessment & Remediation of the Environment, P.O. Box 486, Salisbury South, SA 5106, Australia.

E Cotton Catchment Communities CRC, Australian Cotton Research Institute, Locked Bag 1001, Narrabri, NSW 2390, Australia.

F Corresponding author. Email: maria.lategan@uts.edu.au

Marine and Freshwater Research 61(3) 351-356 https://doi.org/10.1071/MF09144
Submitted: 18 June 2009  Accepted: 6 September 2009   Published: 29 March 2010

Abstract

The cotton strip assay uses the loss of tensile strength of cotton strips as a measure of microbial cellulolytic activity. Its suitability for measuring general microbial activity in groundwater was tested by examining the relationship of tensile strength, abundance of cellulolytic organisms and general microbial activity on cotton strips deployed in bores. The hypothesis was that the strength of cotton strips would decline with increasing abundance and activity of cellulolytic organisms, and as cellulolysis makes resources available to other microbial groups, cotton strength loss should also be related to increased overall microbial activity. The correlation between the abundance of cellulolytic organisms and cotton strength was not significant. Two main factors influenced this relationship: (i) effectiveness of the media in detecting cellulolytic moulds and (ii) inter-community interactions. After accounting for the presence of moulds through partial correlation, the relationship between tensile strength and abundance of cellulolytic organisms was stronger and significant. Both cotton strength and abundance of cellulolytic organisms correlated significantly with general microbial activity. These results support the use of the cotton strip assay, and cotton tensile strength as a surrogate for microbial activity in groundwater.

Additional keywords: aquifers, fluorescein diacetate.


Acknowledgements

The authors gratefully acknowledge Kerrie Hannigan and Norman Booth for technical support. We thank Ipek Kurtboke, Queensland University, for guidance in the isolation of cellulolytic organisms from environmental samples. We also thank Peter Hancock for suggesting the use of cotton strips for detecting microbial activity in aquifers and Andrew Boulton for comments on the manuscript. M.J.L. was supported by CRC CARE. K.K. was supported by Cotton Catchment Communities CRC. We are grateful to NSW Department of Water and Energy, particularly Stuart Matthews, for access to monitoring bores. Water samples were kindly analysed by the NSW Department of Water and Energy Analytical Laboratory. The authors thank Matthew Slocum and an anonymous reviewer for their constructive comments.


References

Adam, G. , and Duncan, H. (2001). Development of a sensitive and rapid method for the measurement of total microbial activity using fluorescein diacetate (FDA) in a range of soils. Soil Biology & Biochemistry 33, 943–951.
| Crossref | GoogleScholarGoogle Scholar | CAS | APHA (1998). ‘Standard Methods for the Examination of Water and Waste Water’. (American Public Health Association: Washington, D.C.)

Battin, T. J. (1997). An assessment of the fluorescein diacetate hydrolysis as a measure of total biofilm activity in streambed sediments. The Science of the Total Environment 198, 51–60.
| Crossref | GoogleScholarGoogle Scholar | CAS | French D. D. (1988). Seasonal patterns in cotton strip decomposition in soils. In ‘Cotton Strip Assay: An Index of Decomposition in Soils’. (Eds A. F. Harrison, P. Latter and D. W. H. Walton.) pp. 46–59. (Institute of Terrestrial Ecology: Grange-over-Sands.)

French, D. D. , and Howson, G. (1982). Cellulose decay rates measured by a modified cotton strip method. Soil Biology & Biochemistry 14, 311–312.
| Crossref | GoogleScholarGoogle Scholar | Gillespie J., Latter P. M., and Widden P. (1988). Cellulolysis of cotton by fungi in 3 upland soils. In ‘Cotton Strip Assay: An Index of Decomposition in Soils’. (Eds A. F. Harrison, P. Latter and D. W. H. Walton.) pp. 60–67. (Institute of Terrestrial Ecology: Grange-over-Sands.)

Haack, S. K. , and Fogarty, L. R. (2004). Spatial and temporal changes in microbial community structure associated with recharge-influenced chemical gradients in a contaminated aquifer. Environmental Microbiology 6, 438–448.
| Crossref | GoogleScholarGoogle Scholar | CAS | PubMed | Howard P. J. A. (1988). A critical evaluation of the cotton strip assay. In ‘Cotton Strip Assay: An Index of Decomposition in Soils’. (Eds A. F. Harrison, P. Latter and D. W. H. Walton.) pp. 35–42. (Institute of Terrestrial Ecology: Grange-over-Sands.)

Howson G. (1988). Current method for preparation, insertion and processing of cotton strips. In ‘Cotton Strip Assay: An Index of Decomposition in Soils’. (Eds A. F. Harrison, P. Latter and D. W. H. Walton.) pp. 166–171. (Institute of Terrestrial Ecology: Grange-over-Sands.)

Kalmokoff, M. L. , and Teather, R. M. (1997). Isolation and characterisation of a bacteriocin from the ruminal anaerobe Butyrivibrio fibrisolvens AR10: evidence for the widespread occurrence of bacteriocin-like activity among ruminal isolates of B. fibrisolvens. Applied and Environmental Microbiology 63, 394–402.
|  CAS | PubMed | Latter P. M., and Walton D. W. H. (1988). The cotton strip assay for cellulose decomposition studies in soil: history of the assay and development. In ‘Cotton Strip Assay: An Index of Decomposition in Soils’. (Eds A. F. Harrison, P. Latter and D. W. H. Walton.) pp. 7–10. (Institute of Terrestrial Ecology: Grange-over-Sands.)

Latter, P. M. , Bancroft, G. , and Gillespie, J. (1988). Technical aspects of the cotton strip assay in soils. International Biodeterioration 24, 25–47.
| Crossref | GoogleScholarGoogle Scholar | Maltby E. (1988). Use of cotton strip assay in wetland and upland environments - an international perspective. In ‘Cotton Strip Assay: An Index of Decomposition in Soils’. (Eds A. F. Harrison, P. Latter and D. W. H. Walton.) pp. 140–154. (Institute of Terrestrial Ecology: Grange-over-Sands.)

Nachimuthu, G. , King, K. , Kristiansen, P. , Lockwood, P. , and Guppy, C. (2007). Comparison of methods for measuring soil microbial activity using cotton strips and a respirometer. Journal of Microbiological Methods 69, 322–329.
| Crossref | GoogleScholarGoogle Scholar | PubMed | R Development Core Team (2008). ‘R: A Language and Environment for Statistical Computing.’ (R Foundation for Statistical Computing: Vienna, Austria.)

Russell, J. B. (1985). Fermentation of cellodextrins by cellulolytic and non-cellulolytic rumen bacteria. Applied and Environmental Microbiology 49, 572–576.
|  CAS | PubMed | Smith R. N., and Maw J. M. (1988). Relationships between tensile strength and increase in metabolic activity on cotton strips. In ‘Cotton Strip Assay: An Index of Decomposition in Soils’. (Eds A. F. Harrison, P. Latter and D. W. H. Walton.) pp. 55–59. (Institute of Terrestrial Ecology: Grange-over-Sands.)

Smith, V. R. , Steenkamp, M. , and French, D. D. (1993). Soil decomposition potential in relation to environmental factors on Marion Island (sub-antarctic). Soil Biology & Biochemistry 25, 1619–1633.
| Crossref | GoogleScholarGoogle Scholar |

Suberkropp, K. , Gessner, M. O. , and Chauvet, E. (1993). Comparison of ATP and ergosterol as indicators of fungal biomass associated with decomposing leaves in streams. Applied and Environmental Microbiology 59, 3367–3372.
|  CAS | PubMed |

Tiegs, S. D. , Langhans, S. D. , Tockner, K. , and Gessner, M. O. (2007). Cotton strips as a leaf surrogate to measure decomposition in river floodplain habitats. Journal of the North American Benthological Society 26, 70–77.
| Crossref | GoogleScholarGoogle Scholar |

Treonis, A. , Wall, D. , and Virginia, R. (2002). Field and microcosm studies of decomposition and soil biota in a cold desert soil. Ecosystems 5, 159–170.
| Crossref | GoogleScholarGoogle Scholar | CAS |

Wedekind, K. J. , Mansfield, H. R. , and Montgomery, L. (1988). Enumeration and isolation of cellulolytic and hemicellulolytic bacteria from human faeces. Applied and Environmental Microbiology 54, 1530–1535.
|  CAS | PubMed |

Widden, P. , Howson, G. , and French, D. D. (1986). Use of cotton strips to relate fungal community structure to cellulose decomposition rates in the field. Soil Biology & Biochemistry 18, 335–337.
| Crossref | GoogleScholarGoogle Scholar |