Reproduction, Fertility and Development
Volume 30 Number 1 2018
Proceedings of the Annual Conference of the International Embryo Technology Society, Bangkok, Thailand, 13–16 January 2018
Full Papers and Abstracts for Poster Presentation
RDv30n1Ab11 Microinjection of CPE-Binding Protein Polyadenylated mRNA Increases Developmental Competence of Bovine Oocytes In Vitro
RDv30n1Ab22 Response to Treatment with Human Chorionic Gonadotropin on Pregnancy Rate of Heat-Stressed Lactating Cows: Interactions with Parity and Genotype
RDv30n1Ab44 Relationship Between Ovarian Vascularity, Cumulus–Oocyte Morphology and Luteal Development in Four-Month-Old Calves After FSH Stimulation
RDv30n1Ab55 Live Offspring Produced from Reproductive Material Recovered During the Annual Cull of Bison from Yellowstone National Park
RDv30n1Ab66 CRISPR-Generated ZP4 Knockout Rabbits Exhibit Impaired In Vivo Pre-Implantation Development
RDv30n1Ab77 Ovarian Follicular Dynamics and Conception Rate in Bos indicus Cows with Different Antral Follicle Counts Subjected to Timed Artificial Insemination
RDv30n1Ab88 Effect of Single Layer Centrifugation with Percoll Plus® of Fresh Bull Semen on Plasma Membrane Stability and Lipid Peroxidation After Cryopreservation
RDv30n1Ab1010 Evaluation of Frozen Sperm Quality After Single Layer Centrifugation with Percoll Plus® of Fresh Bull Semen
RDv30n1Ab1111 Early Induction of Luteolysis in Fixed-Time Artificial Insemination Protocols Increases Fertility in Beef Cows
RDv30n1Ab1212 Ovulation Timing Following an Aromatase Inhibitor-Based Synchronization Protocol in Beef Heifers and Cows
RDv30n1Ab1313 Pre-synchronization by Induction of a Dominant Follicle Using a Progesterone Device in a GnRH-Based-Ovulation Synchronization Protocol in Lactating Dairy Cows
RDv30n1Ab1414 Composition of Semen Extenders and Varying Seminal Plasma Concentrations Affects Formation of Neutrophil Extracellular Traps in the Bovine System
RDv30n1Ab1515 Effects of Timed Artificial Insemination Protocol Using Prostglandin F2α and Estradiol Benzoate on Conception Rate in High-Producing Dairy Cows
RDv30n1Ab1616 Evaluation of Factors Influencing Timed Artificial Insemination in South African Communal Cows
RDv30n1Ab1818 Wireless Cloud-Based Data Acquisition and Management Tool for Use with Inteli-Straws and Assisted Reproductive Techniques
RDv30n1Ab1919 An Efficient Noninvasive Alternative Recipient Preparation in Swamp Buffalo Using the Ovsynch Protocol
RDv30n1Ab2020 Assessment of Motion and Kinematic Characteristics of Semen from Four Cattle Breeds Using Computer-Aided Sperm Analysis
RDv30n1Ab2121 Comparative Study on Epididymal Spermatozoa Traits of Large White × Landrace and Kolbroek Boars
RDv30n1Ab2222 Comparing Three Extenders: Hashi, Green Buffer and INRA 96, for Chilled Storage of Bactrian Camel Semen
RDv30n1Ab2424 Evaluation of Latrunculin A for the Activation of Hand-Made Cloning (HMC) Porcine Embryos
RDv30n1Ab2525 Rescue of the Hematoendothelial Phenotypes in the ETV2-Null Cloned Pig via Embryo Complementation
RDv30n1Ab2727 Quisinostat, a Potent Histone Deacetylase Inhibitor, Regulates the Expression of Pluripotency- and Reprogramming-Related Genes on Somatic Cell Nuclear Transferred Porcine Embryos
RDv30n1Ab2828 Generation of Immunoglobulin Heavy Constant Mu (IGHM) Knockout Goats Using CRISPR/Cas9 and Somatic Cell Nuclear Transfer
RDv30n1Ab3232 MicroRNA-29b Improves the Quality and Developmental Potential of Blastocysts Derived from Somatic Cell Nuclear Transfer in Cattle
RDv30n1Ab3535 Interspecies Somatic Cell Nuclear Transfer Embryos that Form Nucleoli do not Always Activate Mitochondrial Functional Differentiation at the Time of Embryonic Genome Activation
RDv30n1Ab3737 Healthy Foals Produced Using Bone Marrow-Mesenchymal Stem Cells as Nuclear Donors in Horse Cloning
RDv30n1Ab3838 Improved Cloning Efficiency and Developmental Potential in Bovine Somatic Cell Nuclear Transfer with the New Technology
RDv30n1Ab4040 Gene Expression Profiling of In Vitro-Produced Blastocysts Derived from In Vitro-Matured Bovine Oocytes Vitrified/Warmed in Media Supplemented with a Biopolymer Produced by an Antarctic Bacterium
RDv30n1Ab4141 Effects of Dimethyl Sulfoxide- or Glycerol-Based Vitrification Protocols on Zona Pellucida Hardening in Mature Bovine Oocytes
RDv30n1Ab4444 Proliferation and Antral Formation of Preantral Follicle Within Cryopreserved Cat Ovarian Tissue Transplanted into Nude Mice
RDv30n1Ab4545 DNA Fragmentation of Epididymal Freeze-Dried Ram Spermatozoa Impairs Embryo Development
RDv30n1Ab4646 Effect of Bioxcell® and Triladyl® Extenders on Washed Semen of South African Indigenous Bucks
RDv30n1Ab4747 A New Device and Method for Successful Vitrification of In Vitro-Produced Ovine Embryos
RDv30n1Ab4848 Effect of Dimethyl Sulfoxide- or Glycerol-Based Vitrification Protocols on Meiotic Spindle of In Vitro-Matured Bovine Oocytes
RDv30n1Ab4949 Developmental Potential of Dromedary Camel Oocytes Vitrified at the Germinal Vesicle Stage: Effects of Different Cryoprotectant Combinations and Cryo-Carriers
RDv30n1Ab5050 Effects of Cathepsin B Inhibitor E64 on the Survival Rate of Cryopreserved Semen from Korean Brindled Bulls
RDv30n1Ab5151 Effect of Cryoprotectant Exposure Time on Development of Vitrified-Warmed Immature Equine Oocytes
RDv30n1Ab5555 The Role of Poly (ADP-Ribose) Polymerases in Porcine Cumulus–Oocyte Complex During In Vitro Maturation and Embryonic Development
RDv30n1Ab5656 Sirtinol Treatment Influences Preimplatation Development of Porcine Embryos via Regulation of Autophagy and Apoptosis
RDv30n1Ab5757 Disruption of TET1 Leads to Abnormal Expression of Pluripotency-Related Genes in Porcine Embryos
RDv30n1Ab5858 Evidence of Metabolic Compartmentalization or Zonation in the Bovine Placenta: Significance for the Regulation of Placental Function and Fetal Growth
RDv30n1Ab5959 Selected Reaction Monitoring-Based Absolute Quantification of Developmentally Relevant Proteins in Early Bovine Embryos Reveals Differences Between In Vitro and In Vivo Embryo Culture and Between Different Maternal Metabolic Stages
RDv30n1Ab6060 Reproductive Performance After Timed Artificial Insemination Followed by Timed Embryo Transfer of In Vitro-Produced Embryos in Beef Cattle
RDv30n1Ab6262 Will Gonadotropin-Releasing Hormone Treatment Hasten the Onset of Puberty in Peripubertal Heifers?
RDv30n1Ab6464 Impaired Post-Implantation Development Following Blastomere Biopsy is Associated with Placental Hypomethylation
RDv30n1Ab6565 Placental Function at Term is Altered in Broodmares Fed with Cereals from Mid Gestation
RDv30n1Ab6666 Unexpected Protein Dynamics During the Oocyte-to-Embryo Transition in Mice: a Mass Spectrometry and RNA Sequencing Tandem Study
RDv30n1Ab6767 Influence of Metabolic Status and Genetic Merit for Fertility on Proteomic Composition of Bovine Uterine Luminal Fluid
RDv30n1Ab6969 Can Bovine Sperm Interaction with the Oviduct Cells After Artificial Insemination Affect the Transcriptome Profile of the Oviduct?
RDv30n1Ab7070 Effect of Ovulation Stimulus on Biochemical Composition of the Oviduct of Alpaca (Vicugna pacos)
RDv30n1Ab7171 The Effect of Two Different In Vitro Culture Media and Mice Embryo Groupings on Hatchability After 24 Hours of Culture
RDv30n1Ab7272 Protective Effects of C-Phycocyanin on the Developmental Competence of Porcine Parthenotes
RDv30n1Ab7373 Fatty Acid Supplementation in Culture Medium with Reduced Nutrient Concentrations Improves Bovine Blastocyst Development Compared with Standard Culture Medium
RDv30n1Ab7474 Treatment with Melatonin During In Vitro Culture Enhances Porcine Parthenogenetically Activated Embryo Development
RDv30n1Ab7575 Improvement of Developmental Competence of Bovine In Vitro-Produced Embryos by Using Charcoal:Dextran-Stripped Fetal Bovine Serum on In Vitro Culture Media
RDv30n1Ab7676 Tetrahydrofuran does not have Embryo Toxic Effects on In Vitro-Produced Bovine Embryos
RDv30n1Ab7777 Monozygotic Twin Calves Production by Blastomere Separation Technique with Commercial Well-of-the-Well Culture Dish
RDv30n1Ab7878 Mito-TEMPO, a Scavenger for Mitochondria-Derived Reactive Oxygen Species, Enhances Porcine Pre-Implantation Embryo Development
RDv30n1Ab7979 Effect of Polydatin on Development and Cryotolerance of In Vitro-Produced Bovine Embryos
RDv30n1Ab8080 Cumulus Cells Reflect the Success of IVF of Porcine Oocytes Treated with Dimethylthiourea
RDv30n1Ab8181 Effects of Day 5 or 6 HEPES-Buffered Transportation Culture Medium on Developmental Competence of Bovine In Vitro-Produced Embryos
RDv30n1Ab8282 Compensation of the Growth and Development of Individually Transferred Bovine Bisected Embryos
RDv30n1Ab8383 Effect of Synchronization Treatment and Estrus Expression on Conception Rates and Pregnancy Losses in Recipients Receiving In Vitro-Produced Embryos
RDv30n1Ab8484 Effect of Embryo Stage and Cryopreservation Method on Pregnancy Rates Obtained Following the Transfer of In Vivo-Derived Ovine Embryos on Small-Scale Farms in Thailand
RDv30n1Ab8686 Difficulty of Transfer of In Vivo-Derived Bovine Embryos and Route of Administration of Flunixin Meglumine at the Time of Transfer may Affect Pregnancy Rate
RDv30n1Ab8787 Effect of Treatment with Nonsteroidal Anti-Inflammatory Drugs (NSAIDs) on Pregnancy Rates of Recipient Alpacas Post-Embryo Transfer
RDv30n1Ab8989 Comparative Quantification of Plasma Progesterone Through Radioimmumoassay and Enzyme-Linked Fluorescent Assay Techniques in Cattle
RDv30n1Ab9090 Risk of Coxiella burnetii Transmission via Embryo Transfer Using In Vitro Early Caprine Embryos
RDv30n1Ab9191 Expression Pattern of Neuron-Specific Red Fluorescence Protein in Nervous System of Transgenic Dog with Human Synapsin I Promoter
RDv30n1Ab9292 Sperm Cryopreservation in the Burmese Python (Python bivittatus) as a Model for Endangered Snakes
RDv30n1Ab9494 Semen Parameters from a Captive Population of the Endangered Chacoan Peccary (Catagonus wagneri) in Paraguay
RDv30n1Ab9696 Follicular Fluid and Serum Biochemical Composition in Alpacas (Vicugna pacos) with Different Nutritional Planes
RDv30n1Ab9898 Assessing Endangered Felid Puma concolor Sperm Fertility by In Vitro Fertilization with Domestic Cat Oocytes
RDv30n1Ab100100 Use of Seminal Plasma, Human Chorionic Gonadotropin, and Follicular Ablation on the Interval to Follicular Wave Emergency and Dominant Follicle in Alpacas (Vicugna pacos)
RDv30n1Ab101101 Effects of Active Immunization Against GnRH in Oocyte Donors with Cystic Ovarian Disease
RDv30n1Ab102102 In Vitro Embryo Production and Oocyte Quality in Bos indicus Beef Cows Selected for Fertility Characteristics
RDv30n1Ab103103 The Tunica Albuginea Increases in Thickness and Density Correlatively with the Age of the Patient in Human Ovaries
RDv30n1Ab104104 Distribution of Gonadotropin-Releasing Hormone and Kisspeptin Neurons in the Preoptic Area and Hypothalamus During the Estrous Cycle in Cows
RDv30n1Ab106106 Effect of Heat-Stress on Antral Follicle Count and Serum Concentration of Anti-Mullerian Hormone in Bos taurus Crossbred Beef Cows
RDv30n1Ab107107 Localization of Kisspeptin Immunoreactivity in the Cat Ovary on Different Reproductive Stages
RDv30n1Ab108108 Single-Cell RNA Sequencing Reveals Blastomere Heterogeneity and Early Lineage Specification Events in Bovine Embryos During Major Embryonic Genome Activation
RDv30n1Ab109109 Dosage Compensation of the X Chromosome in Ovine Embryos, Late Gestation, and Adult Somatic Tissues
RDv30n1Ab110110 Temporal Changes in Endometrial Gene Expression Between Ipsi- and Contralateral Uterine Horns in Cattle
RDv30n1Ab112112 Identification of Solute Carrier Family 5 Member 9 (SLC5A9) as Biomarker of In Vivo and In Vitro Oocyte Developmental Competence
RDv30n1Ab113113 Specific Impacts of Mild Feed Restriction on Gene Expression of Endometrial Luminal, Glandular and Stromal Cells in Postpartum Dairy Cows
RDv30n1Ab114114 Insulin-Like Growth Factor (IGF)2 and IGF2 Receptor (IGF2R) Murine Blastocyst Transcriptional Response after High Gaseous Pressure Exposure at 8-Cell Stage
RDv30n1Ab115115 In Vivo Transcriptomic Response of Bovine Oviduct Epithelial Cells to the Early Embryo
RDv30n1Ab116116 In Vitro Transcriptomic Response of Bovine Oviduct Epithelial Cells to Direct or Indirect Embryo Contact
RDv30n1Ab119119 Genetic Highlights for Reproduction and Health Traits in Russian Black-and-White and Holstein Animals Selected for Production of High-Quality Embryos
RDv30n1Ab123123 In Vitro Embryo Production in Lyophilized In Vitro Culture Medium as a Method to Increase the Medium's Shelf-Life
RDv30n1Ab124124 Cell Count of Bovine In Vitro-Produced Blastocysts After In Vitro Maturation in Glass versus Plastic Vials
RDv30n1Ab126126 A Comparison of Two Different Follicular Coasting Periods for In Vitro Embryo Production in Indian Nelore Cattle
RDv30n1Ab127127 L-Carnitine Supplementation During In Vitro Maturation Improves Developmental Competence of Canine Oocytes
RDv30n1Ab128128 Evidence that Pregnancy-Associated Serum Protein A (PAPP-A) Plays Role on Bovine In Vitro Embryo Production
RDv30n1Ab129129 Embryo Production from Fully Grown and Growing Stage Oocytes in Japanese Black Calves
RDv30n1Ab130130 In Vitro Production of Hybrid Desert Bighorn×Domestic Sheep Embryos Using Frozen–Thawed Epididymal Semen from a Hunter-Harvested Ram
RDv30n1Ab131131 Effect of Oviductal Fluid During In Vitro Culture on Bovine Embryo Development and Quality
RDv30n1Ab132132 The Developmental Competence of Bovine Oocytes Exposed to Progesterone and Luteotrophic Hormones During the Second Step of Two-Step Maturation
RDv30n1Ab133133 In Vitro Embryo Production from Oocytes Collected from Non-Supertimulated Wood Bison (Bison bison athabascae) Following Maturation In Vitro Using Portable Incubators
RDv30n1Ab134134 Effects of Gas Tension During Culture upon Development of Bovine Embryos from Beef (Nellore) and Dairy (Girolando) Breeds
RDv30n1Ab135135 Effect of Different Bovine Breeds on Efficiency of Embryo Production by Ovum Pick-Up-IVF
RDv30n1Ab136136 DNA Methylation Status and Transcript Profile of Genes Associated with Lipid Metabolism, Cell Survival, and Pluripotency in Bovine Blastocysts with Different Kinetics of Development
RDv30n1Ab137137 Kinetics of In Vitro Embryonic Development According to the Follicular Population in Bos Indicus
RDv30n1Ab138138 Relationships Between Antral Follicle Count, Ovarian Volume, Pre-Antral Follicle Number, and Oocyte Quality
RDv30n1Ab139139 Sperm Transmembrane Protein 95 (TMEM95) is Required for Sperm–Oocyte Interaction and Successful Fertilization but not Mucus Penetration in Cattle
RDv30n1Ab141141 Antioxidant Supplementation Alleviates DNA Damage in Boar Sperm Induced by Tropical Heat Stress
RDv30n1Ab145145 Effect of Dietary Organic Zinc Levels on Boar (Sus scrofa domesticus) Sperm DNA Fragmentation Dynamics
RDv30n1Ab146146 Effect of pH on Rainbow trout (Oncorhynchus mykiss) Sperm Motility Using Five Extender Solutions
RDv30n1Ab147147 Dietary Zinc and its Effect on Collection Time and Training Quality of Young Boars for Obtaining Ejaculates
RDv30n1Ab148148 Inmunolocalization of β-Nerve Growth Factor (NGF) in Male Reproductive Tract and NGF Levels in Serum and Seminal Plasma at Puberty and Adulthood in Rabbit
RDv30n1Ab149149 Preliminary Results of the Oxytocin Treatment on the Collection of Cat Semen by Urethral Catheterization After Pharmacological Induction
RDv30n1Ab150150 Runs of Homozygosity Analysis and their Possible Influence on Sperm Motility in Highly Consanguineous Bulls
RDv30n1Ab151151 Associations of Testicular Size and Arterial Blood Flow with Sexual Development and Semen Characteristics in Dorper Rams Raised in a Subtropical Climate
RDv30n1Ab153153 Fertilisation of Cattle Oocytes is Linked to Novel Waves of a Ca2+ Fluorophore in Cumulus Cells
RDv30n1Ab155155 Oocyte Maturation in Lyophilized In Vitro Maturation Medium as a Method to Increase the Medium’s Shelf-Life
RDv30n1Ab158158 The Effect of Follicular Wave Phase at Time of Ovum Pick-Up on Bovine Oocyte Cytoplasmic Maturation and Developmental Competence
RDv30n1Ab159159 Effect of Commercial Embryo Holding Medium on Development and Quality of Immature Bovine Oocytes
RDv30n1Ab161161 Cumulus Cell Luteinization is Enhanced During Aging of Bovine Oocytes Matured In Vitro
RDv30n1Ab162162 Supplementation of Fibroblast Growth Factor 2, Leukemia Inhibitory Factor, and Insulin-Like Growth Factor 1 (FLI) Enables Efficient Production of Competent Porcine Oocytes Without Gonadotropins in the Maturation Medium
RDv30n1Ab163163 Treatment of Growth Differentiation Factor 8 (GDF8) During Porcine Oocyte In Vitro Maturation Changed Transcriptional Pattern and AKT Signaling
RDv30n1Ab164164 The Origin of Oocyte, In Vitro-Matured Oocyte With/Without Super-Stimulation, and In Vivo-Matured Oocyte Influence the Timing of Cleavage in Early Embryo and Oxygen Consumption of Blastocyst After IVF in Japanese Black Cow
RDv30n1Ab165165 Effect of Dimethyl Sulfoxide Supplementation During In Vitro Maturation on the Genetic Expression Pattern of Bovine Blastocyst
RDv30n1Ab166166 Paracrine Regulation of Epidermal Growth Factor-Like Factors in Oviduct Cells and its Effect on Oocyte Maturation and Subsequent Embryo Development
RDv30n1Ab167167 Effect of Storage Time and Temperature of a Commercial Embryo Holding Medium on the Maturation Kinetics of Immature Bovine Oocytes
RDv30n1Ab168168 The Effect of Prematuration Culture Using Epidermal Growth Factor Receptor Kinase Inhibitor on Nuclear Maturation of Bovine Oocytes and Cumulus Cell Gene Expression
RDv30n1Ab169169 Nuclear Maturation Kinetics and In Vitro Fecundation of Immature Bovine Oocytes Injected into Preovulatory Follicles
RDv30n1Ab170170 Effect of Folic Acid Supplementation on In Vitro Maturation of Oocytes and Folate Cycle
RDv30n1Ab171171 Protective Effect of Sodium Pyruvate Against H2O2-Induced Oxidative Stress in Bovine and Swine Oocytes
RDv30n1Ab172172 Effect of Melatonin on Meiosis Progression and Lipid Content in Bovine Oocytes Matured In Vitro
RDv30n1Ab173173 Size Matters: a Follicle-of-Origin Effect on the Preovulatory Epigenetic Constitution of Germinal Vesicle-Stage Porcine Oocytes
RDv30n1Ab174174 Effects of Melatonin on In Vitro Maturation of Bovine Oocytes and Gene Expression in Cumulus Cells: Preliminary Results
RDv30n1Ab175175 Testis-Specific Protein Y Encoded Copy Number Variation in In Vitro-Produced Bovine Early Embryos
RDv30n1Ab176176 Localization and Quantitative Expression of Phospholipase C Zeta in Equine Sperm Using Commercial Antibodies
RDv30n1Ab177177 Equine Sperm Decondensation and Blastocyst Formation After Conventional versus Piezo-Driven Intracytoplasmic Sperm Injection
RDv30n1Ab178178 Differential Behavior of Porcine Mesenchymal Stem Cells from Bone Marrow and Adipose During Osteogenic Differentiation on a Glycosaminoglycan Hydrogel Scaffold for Bone and Cartilage Tissue Engineering
RDv30n1Ab179179 Xenogeneic and Allogeneic Mesenchymal Stem Cell Transplantation for Treatment of Tibial Bone Fracture in Mice
RDv30n1Ab181181 Establishment of Induced Pluripotent Stem Cells from Fishing Cat and Clouded Leopard Using Integration-Free Method for Wildlife Conservation
RDv30n1Ab182182 In Vitro Derivation of Male Germ Cells from Bovine Bone Marrow-Derived Mesenchymal Stem Cells
RDv30n1Ab183183 In Vitro Generation and Characterization of Putative Primordial Germ Cells Derived from Induced Pluripotent Stem Cells in Cattle
RDv30n1Ab184184 Establishment of Porcine Induced Pluripotent Stem Cell Lines by Adding LIN 28 Transcription Factor
RDv30n1Ab185185 Directed Differentiation of Porcine Induced Pluripotent Stem Cells into all Three Germ Layers via Embryoid Body Formation
RDv30n1Ab186186 Improving Success Rate of Establishment and Maintenance of Porcine Induced Pluripotent Stem Cells by Investigation of Colony Morphology
RDv30n1Ab187187 Morphological Appearance and Expression of Spermatogonial Stem Cell Markers in White Rhinoceros Testicular Tissue
RDv30n1Ab188188 The Effect of Vitamin B12 on the Differentiation of Adipose-Derived Stem Cells into Osteoblasts
RDv30n1Ab189189 The Effect of Vitamin K on the Differentiation of Adipose-Derived Stem Cells into Osteoblasts
RDv30n1Ab190190 Profile of MicroRNAs of Equine Amniotic Mesenchymal Cell-Derived Microvesicles in Different Time-Span of In Vitro Culture
RDv30n1Ab192192 Culture Conditions Supporting Long-Term Expansion of Bovine Spermatogonial Stem Cells Isolated from Adult and Immature Testes
RDv30n1Ab193193 Comparison of Superovulatory Responses to Single Subcutaneous FSH Injection and Twice-Daily Intramuscular FSH Injection Protocols in Japanese Black Cows
RDv30n1Ab194194 Usefulness of the Ultrasound Imaging Modalities B-Mode and Colour Doppler Sonography and Serum Progesterone Concentrations for Determining Superovulatory Ovarian Responses in Ewes
RDv30n1Ab195195 Are the Spectral Doppler Indices of the Ovarian Arteries Predictive of Ovulatory Responses and Embryo Yields in Superovulated Ewes?
RDv30n1Ab196196 Fertilization Rate in Superovulated Criolla Goats Following Artificial Insemination or Natural Mating
RDv30n1Ab197197 The Effect of Gonadotropin-Releasing Hormone Agonist on Superovulation and Estrous Synchronization in Female Sprague Dawley Rat
RDv30n1Ab199199 Efficient Knock-out of Ovine β-Lactoglobulin (BLG) Gene and Knock-in of Recombinant Human Factor IX (rhFIX) Under BLG Native Regulatory Sequences in Somatic Cells and Zygotes Using TALEN Nuclease
RDv30n1Ab202202 Targeting Galactosyl-α-1,3-Galactose (αGal) Epitopes for Multi-Species Embryo Immunosurgery
RDv30n1Ab203203 Genome Modifications by Sleeping Beauty Transposition and CRISPR/Cas9 to Improve Cow Milk Composition for Human Consumption
RDv30n1Ab205205 Optimization of Transfection Efficiency for CRISPR/Cas9-Induced Genomic Editing in Porcine Fibroblast Cells
RDv30n1Ab206206 Effect of L-Ascorbic Acid, Polydatin, and In-Straw Rehydration of Slow-Frozen In Vitro-Produced Bovine Embryos
RDv30n1Ab208208 Cryotolerance of In Vitro-Produced Cattle Embryos Produced with Different Maturation and Culture Media
The peer-reviewed and edited version of record published online before inclusion in an issue
Activin C can delay the growth of mouse granulosa cell tumours, but little is known about its function in normal ovaries. This study shows that activin C protein is expressed in normal mouse and human ovaries, and can delay the development of abnormal follicles in mice lacking inhibin, but does not appear to alter normal follicle growth in the mouse. This study provides evidence of a role for activin C in the ovary.
RD17343Influence of postnatal prolactin modulation on the development and maturation of ventral prostate in young rats
Prostate development and function are altered by early postnatal exposure to prolactin imbalance. Prolactin modulation interferes with the determination of ventral prostate development and maturation through a mechanism of increasing the epithelial proliferative response and dynamics of cell differentiation. Changes in prostate morphophysiology after prolactin modulation could affect glandular function and the incidence of disorders associated with aging.
RD17165De novo transcription of thyroid hormone receptors is essential for early bovine embryo development in vitro
This article contributes to a better understanding of which transcripts are involved in early embryonic development, when the embryos still rely on the maternal genetic component and after they activate their own genome. We identified the thyroid hormone receptor, especially the β-subunit, as one of the few genes transcribed before major embryonic genome activation with an essential role for development.
During preimplantation development, the bovine embryo is free floating in oviduct and uterine fluids. We aimed to mimic the physiological conditions of the maternal environment in the present study by supplementing serum-free embryo culture medium in vitro with both fluids sequentially. The results revealed that low concentrations of oviduct and uterine fluids support early embryo development and improve blastocyst quality.
The zona pellucida (ZP) is an extracellular matrix formed by a few glycoproteins that covers the oocyte and plays an important role during fertilization. The identification of the components of the ZP in marsupials is relevant for the cellular understanding of female gamete formation, sperm interaction with the ZP and fertilisation. Molecular and in silico analyses demonstrated a different ZP composition between Australasian and South American marsupials, being formed from four to six proteins. The present findings have potential relevance from a biological, ecological and economic point of view, related to the development of new contraceptive vaccines for marsupials.
RD17096Effect of exogenous progesterone on embryo size and ewe uterine gene expression in an ovine ‘dam size' model of maternal constraint
The preimplantation period of pregnancy is a critical period for determining growth of the conceptus. This study examined the use of progesterone treatment as a means of overcoming maternal constraint effects on embryo growth. Exogenous progesterone was found to indirectly advance embryo growth via alteration of endometrial genes that encode for uterine structural and secretory activity and thereby overcome maternal constraint.
Nutritional perturbation during early gestation has been shown to alter male reproductive development in rodents and sheep. This study examined the effects of dietary protein perturbation during the peri-conception and first trimester periods upon reproductive development in both fetal and pubertal male bovine progeny. Low maternal dietary protein negatively impacted reproductive development in pubertal and postpubertal offspring. This research highlights that further studies are warranted to explore causal relationships between gestational nutrition and consequent postnatal male reproductive development in cattle.
RD17028Molecular cloning and epigenetic change detection of Kiss1 during seasonal reproduction in Chinese indigenous sheep
The Kiss1 gene plays a major role in reproductive seasonality of sheep. This study aimed to confirm that epigenetic modification occurs in sheep during the reproductive cycle. A potential role of histone acetylation modification in altering Kiss1 expression, which may consequently regulate seasonal breeding in sheep, was found. This study may offer a theoretical basis for breeding year-round oestrous sheep.
RD17292Proteinaceous sperm motility inhibitory factor from the female Indian garden lizard Calotes versicolor
Female sperm storage is known across many animal phyla, but the mechanism(s) remains elusive. Using the Indian garden lizard, Calotes versicolor as a model, we identified a unique abundant protein of ~55-kDa in the uterovaginal region that controls the motility of the spermatozoa. These studies are likely to unravel the secrets of sperm storage.
RD17163Changes in bone turnover and calcium homeostasis during pregnancy and lactation in mammals: a meta-analysis
Due to fetal and neonatal bone tissue calcification, the maternal calcium demand increases during pregnancy and lactation. To supply this calcium demand, female mammals undergo numerous physiological changes during reproduction. In the present study, we performed a meta-analysis of studies reporting changes in bone mineral density, serum calcium and calciotropic hormone concentrations during pregnancy and lactation in mammals. The results provide a quantitative background to better explain and understand these physiological adaptations.
RD17265Effects of N-carbamylglutamate and
l-arginine on gonadotrophin-releasing hormone (GnRH) gene expression and secretion in GT1-7 cells
N-carbamylglutamate (NCG) and arginine (ARG) supplementation can increase litter size in pigs and rats, but their effects on gonadotrophin-releasing hormone (GnRH) secretion are unknown. We found that NCG and ARG can increase cell numbers and decrease GnRH secretion in murine immortalized GT1-7 cells in vitro by regulating related gene expression. These findings help us to better understand nutritional control of reproduction.
The molecules that are involved in actin-based oocyte polar body extrusion are still largely unknown. In the present study we showed that knockdown of Profilin caused aberrant actin distribution, the failure of polar body extrusion and altered the expression of Rho-associated kinase (ROCK) and myosin light chain (MLC) 2. The results indicate that Profilin is related to ROCK–MLC2 for actin assembly in mouse oocytes.
RD17251Seminal plasma and cryopreservation alter ram sperm surface carbohydrates and interactions with neutrophils
The reason why some spermatozoa reach the site of fertilisation where others fail is not known, but interaction between spermatozoa and the female environment is key. We profiled ‘winner’ and ‘loser’ spermatozoa and found that the sugars on their surface and their susceptibility to immune cell attack differed significantly. This helps to explain both normal reproductive physiology and the consequences of human intervention.
RD16309The influence of dopaminergic system inhibition on biosynthesis of gonadotrophin-releasing hormone (GnRH) and GnRH receptor in anoestrous sheep; hierarchical role of kisspeptin and RFamide-related peptide-3 (RFRP-3)
This research provides new insight into central molecular mechanisms regulating GnRH/LH secretion in anestrous sheep. To better explain this problem, herein, we demonstrated that RF-amide peptides are involved in dopamine-induced inhibition of GnRH/LH secretion. Studies expanded an important issue in the field of neuroendocrinology of reproduction, especially in cognitive appraisals of mechanisms participating in physiological regulation of GnRH/LH biosynthesis.
RD17184Expression of vascular endothelial growth factor A isoforms is dysregulated in women with endometriosis
Angiogenesis is a vital process in the development and survival of lesions during endometriosis. In the present study we identified upregulation of several isoforms of vascular endothelial growth factor (VEGF) A in the endometrium of women with endometriosis during menstruation and, for the first time, identified the natural expression of the VEGF111 isoform in humans. These findings contribute to our understanding of how the endometrium differs in women with endometriosis, and indicates a dysregulation at the critical time of menstruation.
Do extracellular vesicles have a biological role in the communication between the embryo and its microenvironment during in vitro culture? In this study we showed that embryos were able to incorporate microvesicles obtained from endometrium and amnion, but only amniotic progenitor cell-derived microvesicles resulted in better blastocyst production rate and quality compared to control. This study could be a useful starting point for further studies related to paracrine mechanisms of communication between embryos and the culture medium.
The role of melatonin as an endocrine regulator of reproductive activity is well known; however, whether it stimulates or inhibits reproductive function varies across species. We demonstrated that in vitro exposure to melatonin reduced steroid production by ovarian theca cells in cattle, implicating melatonin as a regulator of reproductive function in cattle. Because reduced reproductive capacity leads to economic losses, an understanding of the effects of melatonin on ovarian function may lead to new ways to increase reproductive efficiency in cattle.
RD17024Phosphoinositide 3-kinase/protein kinase B (PI3K/AKT) and Janus kinase/signal transducer and activator of transcription (JAK/STAT) follicular signalling is conserved in the mare ovary
Signalling pathways responsible for follicular activation in the mare remain uncharacterised. Messenger RNA expression and protein localisation of members of the PI3K/AKT and JAK/STAT pathways were analysed using tissue from fetal and adult mare ovaries. Findings suggest that members of these pathways are indeed present and may serve as useful biomarkers for assessment of ovary development in the horse.
RD17241Effect of lipopolysaccharide on the expression of inflammatory mRNAs and microRNAs in the mouse oviduct
Uncontrolled inflammation of the oviduct can result in tubal occlusion and infertility. This inflammation is often in response to infection with sexually transmitted bacterial pathogens. This study describes how the endotoxin from Escherichia coli-derived lipopolysaccharide affects the expression of inflammatory mRNAs and post-transcriptionally modifying miRNAs within the oviduct, providing novel insight into the molecular regulation of inflammation within this key reproductive organ.
RD17076Modification of membrane cholesterol and desmosterol in chicken spermatozoa improves post-thaw survival and prevents impairment of sperm function after cryopreservation
During cryopreservation, spermatozoa undergo cryodamage because of membrane changes, including sterol loss, which results in diminished post-thaw fertilisation ability. The present study showed that loading of cholesterol and desmosterol into chicken spermatozoa prior to cryopreservation enhanced their post-thaw quality by inhibiting apoptotic changes and premature acrosome reaction, providing new mechanistic insight into cryodamage in chicken spermatozoa.
RD17049Identification and expression of forkhead box genes in the Chinese giant salamander Andrias davidianus
The forkhead box (Fox) gene family encodes multiple forkhead proteins that exhibit marked functional diversity and are involved in biological processes, especially sex differentiation. The identification, characterisation, selective pressure, protein structure and expression pattern of Fox genes in response to temperature were investigated in the present study in the Chinese giant salamander Andrias davidianus to enrich the sexual development and reproduction mechanism in amphibian.
To improve current infertility treatments, it is necessary to evaluate uterine receptivity in each menstrual cycle. If this can be evaluated beforehand, when uterine receptivity is inadequate eggs could be stored using cryopreservation. In this study in a mouse model, we identified an electrophysiological parameter that can evaluate uterine receptivity prospectively.
Kisspeptin peptide is a major regulator of initiation of pubertal actions in the central nervous system, but its development in growing animals is obscure. The presence of kisspeptin neurons in the brain from birth to puberty in relation to luteinising hormone release pattern was analysed in female lambs. Activity of kisspeptin neurons increased gradually parallel with augmentation of luteinising hormone release and reached maturation several weeks before puberty. The participation of kisspeptin in the development of the ovine reproductive processes is suggested.
RD17255Systemic adiponectin treatment reverses polycystic ovary syndrome-like features in an animal model
Adiponectin may be a potent therapeutic agent for recovery of the polycystic ovary syndrome (PCOS) condition. The aim of the present study was to unravel the role of adiponectin in regulating reproduction, metabolism and fertility status in mice in which PCOS had been induced. Reversal of PCOS-like features following adiponectin treatment in mice is a major breakthrough with regard to the effective management of PCOS.
RD16486Insulinotropic nucleobindin-2/nesfatin-1 is dynamically expressed in the haemochorial mouse and human placenta
The placenta is a multifunctional link between mother and fetus vital for nutrient transport. In this study we determined when and where nucleobindin-2/nesfatin-1 were located in the placenta and found them particularly in secretory trophoblast cells of the mouse and human placenta. The placenta should be considered a source for nucleobindin-2/nesfatin-1 during pregnancy, with possible roles in maternal glucose control and nutrient sensing.
RD17044Association of the cysteine-rich secretory protein-3 (CRISP-3) and some of its polymorphisms with the quality of cryopreserved stallion semen
Some genes are involved in stallion semen tolerance to cryopreservation. This work evaluated the relationship between CRISP-3 protein and some of its single nucleotide polymorphisms, with post-thawing semen quality in stallions. This study demonstrated that different parameters of seminal quality are influenced by the CRISP-3 genotype and the concentration of this protein in seminal plasma.
RD17094Milk-fat globule epidermal growth factor 8 (MFGE8) is expressed at the embryo– and fetal–maternal interface in equine pregnancy
Milk fat globule-EGF factor 8 protein (MFGE8) is a protein that promotes cell to cell adhesion and is thought to play an essential role in embryo implantation in mice and humans. We have described the expression of MFGE8 in the equine uterus and found particular strong expression of the protein by the fetal portion of the placenta. These results imply a role for MFGE8 in maintaining an intact placenta during mare pregnancy.
RD17095Mouse double minute homologue 2 (MDM2) downregulation by miR-661 impairs human endometrial epithelial cell adhesive capacity
A major limitation of IVF success is failure of the embryo to successfully attach to the womb. We have identified a critical new mechanism of communication between the human embryo and endometrium (inner lining of the uterus). Overall it is likely that this bi-directional communication facilitates the critical adhesion process during the initiation of successful pregnancy.
The types and copy numbers of very short so-called miRNA molecules were measured in eggs and embryos of cattle. Results highlight the importance of a non-canonical miRNA during stages before embryonic transcription begins, the influence of the parental origin of miRNAs and the importance of measuring absolute as opposed to relative levels of these molecules.
RD17146Spermatogenic phenotype of testis-specific protein, Y-encoded, 1 (TSPY1) dosage deficiency is independent of variations in TSPY-like 1 (TSPYL1) and TSPY-like 5 (TSPYL5): a case-control study in a Han Chinese population
The testis-specific protein, Y-encoded, 1 (TSPY1) gene is a newly confirmed regulator of sperm production, and dosage deficiency of the multicopy gene confers increased risk of spermatogenic failure. The aim of the present study was to evaluate whether the autosomal homologous genes originating from TSPY1 modify the spermatogenic phenotype of TSPY1. The results suggest that phenotypic expression of TSPY1 defects is independent of variations TSPY-like 1 (TSPYL1) and TSPY-like 5 (TSPYL5), highlighting the significance of TSPY1 in the modulation of spermatogenic efficiency.
RD16399Production of inbred offspring by intracytoplasmic sperm injection of oocytes from juvenile female mice
Intracytoplasmic sperm injection (ICSI) has been successfully applied to hybrid mice, but not to inbred mice due to low efficiency. Adding an activation step to ICSI of oocytes from juvenile mice results in the birth of healthy inbred pups. The results of this study show that the modified protocol can potentially improve the efficiency of ICSI in inbred mice.
A prolonged inflammatory response to breeding severely decreases fertility in horses. The aim of the present study was to evaluate differences between fertile and subfertile mares in the expression of selected genes associated with the immune response. Three of the genes analysed proved to be very sensitive and specific diagnostic markers predicting prolonged inflammation, and likely reduced fertility, prior to breeding.
Genetic background can help to explain idiopathic cases of recurrent pregnancy loss (RPL), which make up about 50% of total cases. Here, we have evaluated the genetic profiles of women experiencing RPL by analysing polymorphisms in the NOS2, PTGS2 and VEGFA genes. We found a higher incidence of a polymorphic variant in the NOS2 gene in the RPL group, suggesting a role for this gene in RPL risk, possibly due to oxidative stress mechanisms.
RD17037Oral propylene glycol modifies follicular fluid and gene expression profiles in cumulus–oocyte complexes and embryos in feed-restricted heifers
Ovum pick-up and in vitro embryo production can be used in dairy heifers. However, production results are often disappointing when animals are underfed. A short-term dietary supplement with propylene glycol (400 mL of PG or water/drench, daily drenching at 1600 hours for the first 9 days of the oestrous cycle), given to feed-restricted heifers, was able to restore the expression of selected genes in cumulus cells and blastocysts to levels seen in correctly fed heifers.
One of the predominant causes of anoestrus is a low level of ovarian oestrogens. Herein, we investigated the association of the oestrogen biosynthesis regulator CYP19A1 with the risk of anoestrus in buffaloes and found three SNPs. The risk alleles in these SNPs were significantly associated with lower levels of estradiol and antioxidants and higher incidence of oxidative ovarian damage and anoestrus.
RD16518Breeding in the fat-tailed dunnart following ovarian suppression with the gonadotrophin-releasing hormone agonist Lucrin® Depot
The application of genome storage in conservation is severely constrained by our limited access to reliable assisted breeding techniques. This is particularly so for marsupials, in which the corpus luteum, and thus cycling, is not affected by agents used to control ovarian function in eutherian mammals. Herein we report progress on the use of a single-treatment long-acting suppressor of pituitary function to reset cycling and ovulation in a marsupial.
RD17180Can resveratrol attenuate testicular damage in neonatal and adult rats exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin during gestation?
Humans may be exposed to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), an environmental pollutant, via contaminated food, inhalation or skin contact. The effects of TCDD are exerted via binding to aryl hydrocarbon receptors (AhR). In this study we investigated the possible protective effect of resveratrol, an AhR antagonist, against testicular damage caused by TCDD exposure during pregnancy. The findings suggest that TCDD in combination with resveratrol results in more severe toxicity than administration of either drug alone.
RD17051Development of fresh and vitrified agouti ovarian tissue after xenografting to ovariectomised severe combined immunodeficiency (SCID) mice
For wild rodents such as the agoutis, the combination of gonadal tissue cryopreservation and xenotransplantation could help promote germplasm conservation and reproduction, especially in the case of genetically valuable individuals. The present study demonstrates that xenografted agouti ovarian tissue, fresh or vitrified, is able to promote the return of ovarian activity in ovariectomised SCID C57B1/6 mice.
RD16345Changes in urinary androgen concentration indicate that male giant pandas (Ailuropoda melanoleuca) respond to impending female oestrus during and outside the typical spring breeding season
Although giant pandas have been described as mono-oestrus spring breeders, males exposed to oestrous females outside this timeframe exhibit breeding behaviours and interest in mating. Urinary androgens and sperm parameters were quantified for males exposed to females expressing oestrus during spring, autumn, or winter. The data from this study showed a rapid physiological readiness of male giant pandas to mate in response to female oestrous cues within or outside of the normal breeding season and may suggest a facultative seasonal reproduction with a “female-induced rut”.
RD16487In vitro growth and development of isolated secondary follicles from vitrified caprine ovarian cortex
In vitro growth of secondary follicles isolated from the vitrified ovarian cortex allows a better understanding of their complex development and of obtaining healthy follicles. The aim of this study was to investigate how the medium composition affects the viability and growth rate of the isolated secondary follicles. This technique could become a strategy to preserve the reproductive potential of females.
Reduced fetal growth can contribute to a greater risk of later metabolic disease, but the key mechanisms remain unknown. Herein we report findings from a large animal model (sheep) in which fetal growth was restricted by reducing maternal food intake and show that once the offspring become obese, they exhibit increased abundance of molecular markers of metabolic stress in the fat and liver. This could represent a primary mechanism by which growth-restricted offspring are at greater risk of metabolic ill health in later life.
RD17217The contribution of p53 and Y chromosome long arm genes to regulation of apoptosis in mouse testis
Tumor suppressor protein p53, known widely as the guardian of genome preventing cancer formation, is also involved in physiological apoptosis of germ cells in testes. The study used a mouse model to reveal that germ cell apoptosis during the first round of spermatogenesis can be efficiently mediated by p53-independent mechanisms, but activation of these mechanisms requires engagement of multicopy Y-chromosome long arm genes. The role of Y-chromosome genes in regulation of testicular apoptosis was demonstrated for the first time.
It is not known whether female accessory reproductive organs that play a major role in reproduction are vulnerable to chronic stress effects. This study revealed irreversible oxidative stress in the Fallopian tube following short or long-term chronic stress exposure, whereas uterus changes were reversible following short-term exposure but not after long-term exposure. This study indicates that stress-induced reproductive failure may also be due to alterations in the accessory reproductive organs.
RD16536Impact of equine assisted reproductive technologies (standard embryo transfer or intracytoplasmic sperm injection (ICSI) with in vitro culture and embryo transfer) on placenta and foal morphometry and placental gene expression
Assisted reproduction techniques such as in vitro fertilisation and embryo culture have been shown to affect the size and anatomy of the newborn and the placenta in some species, but no information is available on this area in the horse. We evaluated foals and placentas from pregnancies resulting from natural conception, embryo transfer and transfer of in vitro-produced (intracytoplasmic sperm injection) embryos and found no significant differences between groups in size or weight of the foal or placenta and no differences in expression of 17 genes controlling placental function. Our findings suggest that the horse embryo differs from that of ruminants in its sensitivity to the in vitro environment, supporting the continued use of assisted reproduction techniques for commercial foal production.
The testis is the organ essential for spermatogenesis and male fertility. This paper profiles differences in DNA methylation levels during prepubertal porcine testis development, and provides new insights into mammalian testis development and represents a foundation for additional research using the pig as a model animal.
RD17041Quantitative changes of extravillous trophoblast cells in heavy smoker mothers compared with healthy controls
Extravillous trophoblast cells are one of the main elements that are affected by maternal smoking during pregnancy. Quantitative changes in extravillous trophoblast cells could be evidence for the hypothesis that changes in these cells could affect fetal health by changes in normal placentation.
RD16338Calcium-sensing receptor (CASR) is involved in porcine in vitro fertilisation and early embryo development
Fertilisation and development of the mammalian egg is still not well understood, illustrated by low efficiencies of assisted reproductive technologies. The role of a cell membrane receptor for calcium (CASR) was studied, using pig spermatozoa and eggs as models. Significant effects were demonstrated by stimulating this receptor at different steps of the process. These findings are useful for future basic and applied research.
RD17081Markers of protein synthesis are increased in fetal membranes and myometrium after human labour and delivery
Inflammation plays an important role in the initiation of preterm birth, which remains the largest cause of neonatal deaths. In the present study, components of the protein synthesis machinery were significantly increased with labouring compared to non-labouring fetal membranes and myometrium, and blockade of protein synthesis significantly reduced inflammation in the myometrium. These results may provide further insight into the pathways involved in the initiation of preterm birth.
Toll-like receptors (TLRs) contribute to an exclusive protection system in the uterus during the oestrus cycle; however, the importance of TLRs in tissue repair within the involuting uterus is not known. Herein we show that TLRs are constitutively expressed in uterine cells and that the localisation pattern of TLRs in the endometrium varies with structural changes in the involuting uterus. These results suggest that TLRs have a physiological role in the uterine remodelling events during the post partum involution period.
RD17045Unique oestrogen receptor ligand-binding domain sequence of native parrots: a possible link between phytoestrogens and breeding success
Hormones in plants may provide an important trigger for activating breeding activity in Kākāpō, a critically endangered New Zealand parrot. By comparing native and Australian parrots for their ability to respond to steroid hormones, we discovered novel sequences in the receptivity of parrots to oestrogenic compounds. Receptor modeling revealed that altered interactions with plant oestrogens in parrots may enhance the physiological pathways linked to fertility and successful breeding.
RD17068Efficient generation of goats with defined point mutation (I397V) in GDF9 through CRISPR/Cas9
Taking advantages of the clustered regularly interspaced short palindromic repeat (CRISPR)/CRISPR-associated (Cas) 9 system, we achieved a precise single-nucleotide substitution in the growth differentiation factor 9 (GDF9) gene using a single-stranded oligo deoxynucleotide as a homology-dependent repair template with a relatively high efficiency in order to increase litter size in goats. The present study provides an alternative approach for introducing defined point mutations in large animal models for the improvement of significant traits, as well as for the functional validation of key variants.
RD16441Expanded equine cumulus–oocyte complexes exhibit higher meiotic competence and lower glucose consumption than compact cumulus–oocyte complexes
Equine immature oocytes are classified as expanded or compact and are matured in the laboratory to obtain offspring; higher maturation rates are reported for expanded oocytes. The aim of this study was to assess whether differences in the maturation capacity of these oocytes is related to differences in glucose uptake, metabolism or apoptosis. Consistent metabolic and genomic differences were found between expanded and compact oocytes: compact oocytes consumed more glucose but matured less than expanded oocytes. This indicates that the maturation requirements vary for compact and expanded oocytes.
The zona pellucida (ZP) is an extracellular matrix formed by few glycoproteins that covers the oocyte and plays an important role during fertilisation. The identification of ZP glycoproteins in carnivores is relevant for an understanding of sperm–ZP interactions and species specificity. In this study, molecular and in silico analyses demonstrated the presence of the ZP1 gene in four families (Felidae, Mustelidae, Odobenidae and Ursidae), whereas in three families (Canidae, Otariidae and Phocidae) ZP1 is pseudogenised. This information could be relevant for the development of more specific and efficient contraceptive vaccines for carnivores.
RD17174Linoleic (LA) and linolenic (ALA) acid concentrations in follicular fluid of prepubertal goats and their effect on oocyte in vitro maturation and embryo development
Juvenile in vitro embryo transfer (JIVET) can increase the gain rate by reducing the generation interval, but the low quality of these oocytes leads to poor embryo production. In this study we hypothesised that oocyte quality could be related to omega 3 (linolenic acid, ALA) and omega 6 (linoleic acid, LA) concentrations in follicular fluid. No differences were found in ALA and LA concentrations in relation to follicle diameter. The addition of a high LA (omega 6) concentration to the IVM medium impaired oocyte competence to develop up to the blastocyst stage.
Juvenile in vitro embryo transfer (JIVET) can increase the genetic gain rate by reducing the generation interval, but the low quality of the oocytes and higher oxidative levels leads to poor embryo production. In the present study we hypothesised that melatonin could be a suitable tool for improving oocyte competence in juvenile goats. The results show that melatonin is present in the follicular fluid in juvenile goats and its addition to the in vitro maturation medium reduces oxidation and increases embryo development showing its potential role for JIVET.
These articles have been peer reviewed and accepted for publication. They are still in production and have not been edited, so may differ from the final published form.
Recent and Emerging Reproductive Biology Research in Australia and New Zealand: Highlights from the Society for Reproductive Biology Annual Meeting, 2017
Steroid hormone concentrations and body mass are differently affected by polyunsaturated fatty acids during the oestrous cycle in guinea pigs
Patterns of conceptus development and of progesterone concentrations in maternal blood preceding spontaneous early pregnancy failure in mares
In vivo and in vitro strategies to support caprine preantral follicle development after ovarian tissue vitrification
D-Aspartate amends reproductive performance of aged roosters by changing gene expression and testicular histology
Implications of body condition and seasonality on morphological and functional parameters of testes of Myotis nigricans (Chiroptera: Vespertilionidae)
Metabolomic profiling of the bovine oviductal fluid across the estrous cycle using proton nuclear magnetic resonance spectroscopy
Gene Expression and Lymphocyte Population in the Maternal-Fetal Interface in Sheep Pregnancies Established by Somatic Cell Nuclear Transfer
JNJ-7706621 improves the in vitro developmental competence of porcine parthenogenetic activation and somatic cell nuclear transfer embryos
Effect of lemon essential oil (Citrus limon) and its major components (Limonene (R)-(+) and Limonene (S)-(-)) on cryopreservation of bull spermatozoid
Time within reproductive season, but not age or inbreeding coefficient, influences seminal and sperm quality in the whooping crane (Grus americana)
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