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Food, fibre and pharmaceuticals from animals
REVIEW

Application of DNA markers in animal industries

J.-T. Jeon A , J.-H. Lee B , K.-S. Kim C , C.-K. Park D and S.-J. Oh E F
+ Author Affiliations
- Author Affiliations

A Division of Animal Science and Technologies, Gyeongsang National University, Jinju 660-701, Korea.

B Division of Animal Science and Resources, Research Center for Transgenic Cloned Pigs, Chungnam National University, Daejeon 305-764, Korea.

C Department of Animal Science, Chungbuk National University, Cheongju 361-763, Korea.

D Department of Animal Biotechnologies, Kun-Kuk University, Seoul 143-701, Korea.

E Department of Animal Genomics and Bioinformatics, National Livestock Research Institute, Rural Development Administration, Suwon 441-250, Korea.

F Corresponding author. Email: ohsj@rda.go.kr

Australian Journal of Experimental Agriculture 46(2) 173-182 https://doi.org/10.1071/EA05191
Submitted: 5 July 2005  Accepted: 6 February 2006   Published: 3 March 2006

Abstract

The current animal industry is both technology-intensive and globalised. Efficient molecular tools, such as DNA markers, are in demand to strengthen competitive power by maximising the improvement of livestock and obtaining the trust of customers by the verification of product origins. This review describes the present techniques applying DNA markers in the animal industry, with a focus on beef cattle and pigs. Preliminary data from an individual traceability assay for Hanwoo (Korean cattle) using 20 microsatellite markers is described. The potential uses of the assay are demonstrated for several key markers of different traits: for the porcine stress syndrome gene using the RYR mutation; for acid meat using the PRKAG3 mutation; for intramuscular fat using the FABP3 mutation and for fixing the Dominant white allele using KIT duplication. In addition, a possible strategy is suggested to discriminate between pig breeds using mutations of KIT, MC1R, ND2 and the 11-bp insertion in the D-loop of mitochondrial DNA. The industrial application of DNA techniques is limited at present, however, it is expected that DNA markers originating from trait genes, especially those of low-heritability and difficult-to-measure traits, may contribute to maximising the improvement of the major economic traits of animals in the future.

Additional key words: DNA markers, DNA techniques, industrial application, traceability.


Acknowledgments

This work was supported by a grant from BioGreen 21 program, Rural Development Administration, Korea.


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