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RESEARCH ARTICLE

Influence of chlorsulfuron on rhizobial growth, nodule formation, and nitrogen fixation with chickpea

A. Anderson A C , J. A. Baldock B D , S. L. Rogers B , W. Bellotti C and G. Gill C
+ Author Affiliations
- Author Affiliations

A Current address: Department of Environmental Resource Management, University College Dublin, Belfield, Dublin 4, Ireland.

B CSIRO Land and Water, PMB 2, Glen Osmond, SA 5064, Australia.

C Department of Agronomy and Farming Systems, Adelaide University, Roseworthy, SA 5371, Australia.

D Corresponding author; email: Jeff.Baldock@csiro.au

Australian Journal of Agricultural Research 55(10) 1059-1070 https://doi.org/10.1071/AR03057
Submitted: 3 March 2004  Accepted: 30 July 2004   Published: 25 October 2004

Abstract

Sulfonylurea residues have been found to inhibit the growth of some legume crops and pastures in seasons following application. Negative effects of these herbicides on symbiotic nitrogen fixation by legume crops and pastures have been demonstrated. Reductions in nitrogen fixation may result from a direct effect of the herbicide on rhizobial growth and/or an indirect effect on plant growth. In this study the influence of chlorsulfuron on the growth of chickpea rhizobia [Mesorhizobium ciceri (CC1192)], the growth of chickpea plants, and the extent of nodulation and nitrogen fixation by the chickpea/rhizobia symbiosis were examined. In vitro studies (in yeast mannitol broth and a defined medium) showed that chlorsulfuron applied at double the recommended field application rate did not influence the growth of chickpea rhizobia. An experiment using 14C-labelled chlorsulfuron was conducted to determine if rhizobial cells exposed to chlorsulfuron could deliver the herbicide to the point of root infection and nodule formation. Approximately 1% of the herbicide present in the rhizobial growth medium remained with the cell/inoculum material after rinsing with 1/4 strength Ringer’s solution. This was considered unlikely to affect chickpea growth, nodulation, or nitrogen fixation. A pot experiment was used to define the influence of chlorsulfuron on the growth, nodulation, and nitrogen fixation of chickpeas. The presence of chlorsulfuron in the soil reduced the nodulation and nitrogen fixation of the chickpea plants. Pre-exposing rhizobia to chlorsulfuron before inoculating them into pots with germinating chickpea seeds, reduced the number of nodules formed by 51%. Exposure of chickpeas and chickpea rhizobia to chlorsulfuron can adversely affect the formation and activity of symbiotic nitrogen-fixing nodules, even when only the rhizobial inoculant is exposed briefly to the herbicide.


Acknowledgments

The authors thank the CRC for Weed Management Systems for providing scholarship funding to Annette Anderson.


References


Ahmad T, Hafeez FY, Mahmood T, Malik KA (2001) Residual effect of nitrogen fixed by mungbean (Vigna radiata) and blackgram (Vigna mungo) on subsequent rice and wheat crops. Australian Journal of Experimental Agriculture 41, 245–248.
CrossRef |

Bergersen, FJ (Ed.) (1980). ‘Methods for evaluating biological nitrogen fixation.’ (John Wiley and Sons: Chichester, UK)

Beyer EM, Duffy MS, Hay JV, Schlueter DD (1988) Sulfonylurea herbicides. ‘Herbicides: chemistry, degradation and mode of action’. (Eds PC Kearney, DD Kaufman) (Marcel Dekker Inc.: New York)

Brown CM, Dilworth MJ (1975) Ammonia assimilation by Rhizobium cultures and bacteroids. Journal of General Microbiology 86, 39–48.
PubMed |


Brown HM (1990) Mode of action, crop selectivity, and soil relations of the sulphonylurea herbicides. Pesticide Science 29, 263–281.

Chalk PM (1998) Dynamics of biologically fixed N in legume–cereal rotations: a review. Australian Journal of Agricultural Research 49, 303–316.
CrossRef |

Chambers, A (1995). ‘Field crop herbicide information: detailed information on Australian field crop herbicides.’ (Rutherglen Research Institution, Australia: Rutherglen, Vic.)

Devine, M , Duke, SO ,  and  Fedtke, C (Eds) (1993). ‘Physiology of herbicide action.’ (Prentice Hall: New Jersey, USA)

Eberbach P (1993) The effect of herbicides and fungicides on Legume–Rhizobium symbiosis. ‘Pesticide interactions in crop production: beneficial and deleterious effects’. (Ed. J Altman) (CRC Press: London)

Eberbach P, Douglas LA (1989) Herbicide effects on the growth and nodulation potential of Rhizobium trifolii with Trifolium subterraneum L. Plant and Soil 119, 15–23.

Evans MJ, Dickinson JA, Saunders RJ, King EM (1993) Sulfonylureas and annual medic regeneration on high pH soils. ‘Proceedings of the 7th Australian Agronomy Conference’. Adelaide. (The Australian Society of Agronomy: Parkville, Vic.)


Gaston S, Zabalza A, Gonzalez EM, Arrese-Igor C, Aparicio-Tejo PM, Royuela M (2002) Imazethapyr, an inhibitor of the branched-chain amino acid biosynthesis, induces aerobic fermentation in pea plants. Physiologia Plantarum 114, 524–532.
CrossRef | PubMed |

Gillett DL, Holloway RE (1996) The effects of sulphonylurea herbicide on annual medic in alkaline soil. ‘Workshop Paper: Farming Systems Developments’. Adelaide. (CRC for Soil and Land Management: Adelaide, S. Aust.)


Jessop RS, Hetherington SJ, Hoult EH (1984) The effect of soil nitrate on the growth, nodulation and nitrogen fixation of chickpea (Cicer arietinum). Plant and Soil 82, 205–214.

Jettner RJ, Walker SR, Churchett JD, Blamey FPC, Adkins SW, Bell K (1999) Plant sensitivity to atrazine and chlorsulfuron residues in a soil-free system. Weed Research 39, 287–295.
CrossRef |

Kim S, Han S, Vanden Born WH (1997) Effect of chlorsulfuron on assimilate transport: ultrastructural implications. Weed Science 45, 470–473.

Kim S, Vanden Born WH (1997) Carbon allocation and translocation in chlorsulfuron-treated canola (Brassica napus). Weed Science 45, 466–469.

Koopman DJ, Tow PG, Reeves TG, Gibson AH (1995) Soil acidification, chlorsulfuron application and Rhizobium meliloti as factors in lucerne yield decline. Soil Biology and Biochemistry 27, 673–677.
CrossRef |

Lamb, J ,  and  Poddar, A (1992). ‘The grain legume handbook.’ (Grain Legume Handbook Committee: Riverton, S.Aust.)

Lemerle D, Yuan TH, Murray GM, Morris S (1996) Survey of weeds and diseases in cereal crops in the southern wheat belt of New South Wales. Australian Journal of Experimental Agriculture 36, 545–554.

Martensson AM (1992) Effects of agrochemicals and heavy metals on fast-growing rhizobia and their symbiosis with small-seeded legumes. Soil Biology and Biochemistry 24, 435–445.
CrossRef |

Martensson AM, Nilsson AK (1989) Effects of chlorsulfuron on Rhizobium grown in pure culture and in symbiosis with alfalfa (Medicago sativa) and red clover (Trifolium pratense). Weed Science 37, 445–450.

Moyer JR, Esau R, Kozub GC (1989) Chlorsulfuron persistence and response of legumes in an alkaline soil. Journal of Environmental Science and Health B24, 37–56.

Payne, RW (Ed.) (1993). ‘GenStat 5 Release 3 reference manual.’ (Clarendon Press: Oxford, UK)

Peoples MB, Brockwell J, Bergersen FJ (1992) Factors affecting nitrogen fixation: scope for improvement? ‘Transfer of biologically fixed nitrogen to wheat’. (Ed. JF Angus) (GRDC: Canberra, ACT)

Peoples MB, Herridge DF, Ladha JK (1995) Biological nitrogen fixation: An efficient source of nitrogen for sustainable agricultural production? Plant and Soil 174, 3–28.

Powles SB, Preston C, Bryon IB, Jutsum AR (1996) Herbicide resistance: impact and management. Advances in Agronomy 58, 57–93.

Robinson JA, Tiedje JM (1983) Nonlinear estimations of Monod growth kinetics parameters from a single substrate depletion curve. Applied and Environmental Microbiology 45, 1453–1458.
PubMed |


Rogers SL, Froscio SM, Kookana RS, Oliver DP (1998) Degradation of strychnine by pure bacterial cultures isolated from South Australian agricultural soils. Soil Biology and Biochemistry 30, 1623–1626.
CrossRef |

Rost TL (1984) The comparative cell cycle and metabolic effects of chemical treatments on root tip meristems. III. Chlorsulfuron. Journal of Plant Growth Regulation 3, 51–63.

Rovira AD, Neate SM, Warren RA (1993) The effect of glean residues on growth and nodulation of medic in an alkaline dark brown sandy loam. ‘Proceedings of the 7th Australian Agronomy Conference’, Adelaide. (The Australian Society of Agronomy: Parkville, Vic.)


Rupela OP, Saxena MC (1987) Nodulation and nitrogen fixation in chickpea. ‘The chickpea’. (Eds MC Saxena, KB Singh) pp. 163–189. (CAB International: UK)

Schwenke GD, Peoples MB, Turner GL, Herridge DF (1998) Does nitrogen fixation of commercial, dryland chickpea and faba bean crops in north-west New South Wales maintain or enhance soil nitrogen? Australian Journal of Experimental Agriculture 38, 61–70.
CrossRef |

Sprent, JI ,  and  Sprent, P (Eds) (1990). ‘Nitrogen fixing organisms: pure and applied aspects.’ pp. 93–117. (Chapman and Hall: Melbourne, Vic.)

Strong WM, Harbison J, Nielsen RGH, Hall BD, Best EK (1986) Nitrogen availability in a Darling Downs soil following cereal, oilseed and grain legume crops. 1. Soil nitrogen accumulation. Australian Journal of Experimental Agriculture 26, 347–351.

Unkovich MJ, Sanford P, Pate JS (1997) Nitrogen fixation by annual legumes in Australian Mediterranean agriculture. Australian Journal of Agricultural Research 48, 267–293.
CrossRef |

Vincent, JM (Ed.) (1970). ‘A manual for the practical study of the root-nodule bacteria.’ (Blackwell Scientific Publications: Oxford, UK)

Weier, TE , Stocking, CR , Barbour, MG ,  and  Rost, TL (1982). ‘Botany: an introduction to plant biology.’ 6th edn pp. 210–212. (John Wiley and Sons: Brisbane, Qld)








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