Crop and Pasture Science Crop and Pasture Science Society
Plant sciences, sustainable farming systems and food quality
RESEARCH ARTICLE

Genotypic variability in sequence and expression of HVA1 gene in Tibetan hulless barley, Hordeum vulgare ssp. vulgare, associated with resistance to water deficit

Gang Qian A B C D , Zhaoxue Han A C D , Tao Zhao A C , Guangbing Deng A , Zhifen Pan A and Maoqun Yu A E

A Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China.

B Department of Biology, Zunyi Medical College, Zunyi 563003, China.

C Graduate University of the Chinese Academy of Sciences, Beijing 100039, China.

D The first two authors have contributed equally to this paper.

E Corresponding author. Email: yumq@cib.ac.cn

Australian Journal of Agricultural Research 58(5) 425-431 http://dx.doi.org/10.1071/AR06300
Submitted: 6 September 2006  Accepted: 2 March 2007   Published: 11 May 2007

Abstract

Late embryogenesis abundant (LEA) proteins are thought to protect against water stress in plants. Characteristics of sequence and expression of barley gene HVA1, a member of LEA group 3 protein, were investigated in hulless barley (Hordeum vulgare ssp. vulgare), associated with phenotypically diverse drought-tolerant genotypes. Sensitive and tolerant genotypes were identified from Tibetan populations of cultivated hulless barley, based on scores of water loss rate (WLR), maldondialdehyde (MDA), and proline content. The results indicated that lower MDA contents, lower scores of WLR, and higher proline contents were associated with drought-tolerant genotypes in hulless barley. Notably, differential trends of expression patterns were detected among the selected contrasting genotypes, depending on the duration of dehydration stress. The HVA1 gene tended to respond earlier in the tolerance (after 2 h) compared with sensitive genotypes (after 4 h). Results of quantitative real-time PCR indicated that the relative level of HVA1 expression was always higher in tolerant genotypes, rapidly increasing at the earlier stages (after 2–4 h of dehydration). However, HVA1 expressions of sensitive genotypes had a fast increase from 8 to 12 h of stress. Variable numbers of the 11-amino-acid-motif in LEA3 proteins were not consistent with the lines of drought resistance in hulless barley. Molecular characteristic of LEA3 protein in tolerant lines existed in the consistency of Gln32, Arg33, and Ala195 in Tibetan hulless barley. The present study may indicate that the differential HVA1 gene has a functional role in the dehydration tolerance in hulless barley. The authors suggested that the observed variability in sequence and expression of HVA1 could be related to the diverse drought-tolerant genotypes in crops.

Additional keywords: water loss rate, lipid peroxidation, proline, quantitative polymerase chain reaction.


References

Araus JL Slafer GA Reynolds MP Royo C 2002 Plant breeding and drought in C3 cereals: what should we breed for? Annals of Botany 89 925 940 doi:10.1093/aob/mcf049

Aspinall D , Paleg LG (1981) Proline accumulation: physiological aspects. In ‘The physiology and biochemistry of drought resistance in plants’. (Eds L Paleg, D Aspinall) pp. 215–228. (Academic Press: Sydney)

Bartels D , Souer E (2004) Molecular responses of higher plants to dehydration. In ‘Plant responses to abiotic stress’. (Eds H Hirt, K Shinozaki) pp. 9–38. (Springer-Verlag: Berlin)

Bates LS Waldren RP Teare JD 1973 Rapid determination of free proline for water-stress studies. Plant and Soil 39 205 207 doi:10.1007/BF00018060

Bor M Ozdemir F Turkan I 2003 The effect of salts tress on lipid peroxidation and antioxidants in leaves of sugar beet Beta vulgaris L. and wild beet Beta maritima L. Plant Science 164

Bruce WB Edmeades GO Barker TC 2002 Molecular and physiological approaches to maize improvement for drought tolerance. Journal of Experimental Botany 53 13 25
doi:10.1093/jexbot/53.366.13

Cellier F Conejero G Breitler JC Casse F 1998 Molecular and physiological responses to water deficit in drought-tolerant and drought-sensitive sunflower lines (Helianthus annuus L.): accumulation of dehydrin transcripts correlates with tolerance. Plant Physiology 116 319 328 doi:10.1104/pp.116.1.319

Chandra Babu R Zhang JX Blum A David Ho TH Wu R Nguyen HT 2004 HVA1, a LEA gene from barley confers dehydration in transgenic rice (Oryza sativa L.) via cell membrane protection. Plant Science 166 855 862 doi:10.1016/j.plantsci.2003.11.023

Choi DW Zhu B Close TJ 1999 The barley (Hordeum vulgare L.) dehydrin multigene family: sequences, allelic types, chromosome assignments, and expression characteristics of 11 Dhn genes of cv Dicktoo. Theoretical and Applied Genetics 98 1234 1247 doi:10.1007/s001220051189

Clarke JM McCaig TN 1982 Evaluation of techniques for drought resistance in wheat. Crop Science 22 503 506

Close TJ , Choi DW , Venegas M , Salvi S , Tuberosa R , Ryabushkina N , Turuspekov Y , Nevo E (2000) Allelic variation in wild and cultivated barley at the Dhn4 locus, which encodes a major drought-induced and seed protein, DHN4. In ‘8th International Barley Genetics Symposium’. Adelaide, S. Aust. (Ed. S Logue)

Dure L III (1993) Structural motifs in LEA proteins of higher plants. In ‘Response of plants to cellular dehydration during environmental stress’. (Eds TJ Close, EA Bray) pp. 91–103. (American Society of Plant Physiologists: Rockville, MD)

Gal TZ Glazer I Koltai H 2004 An LEA group3 family member is involved in survival of C. elegans during exposure to stress. FEBS Letters 577 21 26
doi:10.1016/j.febslet.2004.09.049

Heath RL Packer L 1968 Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics 125 189 198 doi:10.1016/0003-9861(68)90654-1

Hong B Uknes SJ Ho THD 1988 Cloning and characterization of a cDNA encoding a mRNA rapidly-induced by ABA in barley aleurone layers. Plant Molecular Biology 11 495 506 doi:10.1007/BF00039030

Jain M Mathur G Koul S Sarin NB 2001 Ameliorative effects of proline on salt stress induced lipid peroxidation in cell lines of groundnut (Arachis hypogea L.). Plant Cell Reports 20 463 468

Jayaprakash TL Ramamohan G Krishnaprasad BT Ganeshkumar Prasad TG Mathew MK Udayakumar M 1998 Genotypic variability in differential expression of lea2 and lea3 genes and proteins in response to salinity stress in fingermillet (Eleusine coracana Gaertn) and rice (Oryza sativa L) seedlings. Annals of Botany 82 513 522
doi:10.1006/anbo.1998.0712

Kawasaki S Borchert C Deyholos M Wang H Brazille S Kawai K Galbraith D Bohnert HJ 2001 Gene expression profiles during the initial phase of salt stress in rice. The Plant Cell 13 889 905 doi:10.1105/tpc.13.4.889

Krishnan M Nguen HT Burke JJ 1989 Heat shock protein synthesis and thermal tolerance in wheat. Plant Physiology 90 140 145

Labhilili M Jourdier P Gautier MF 1995 Characterization of cDNAs encoding Triticum durum dehydrins and their expression patterns in cultivars that differ in drought tolerance. Plant Science 112 219 230
doi:10.1016/0168-9452(95)04267-9

Leon D III 1993 A repeating 11-mer amino acid motif and plant desiccation. The Plant Journal 3 393 369

Lopez CG Banowetz GM Peterson CJ Kronstad WE 2003 Dehydrin expression and drought tolerance in seven wheat cultivars. Crop Science 43 577 582


Ludlow MM Muchow RC 1990 A critical evaluation of traits for improving crop yields in water-limited environments. Advances in Agronomy 43 107 153


Mohsenzadeh S Malboobi MA Razavi K Farrahi-Aschtiani S 2006 Physiological and molecular response of Aeluropus lagopoides (Poaceae) to water deficit. Environmental and Experimental Botany 56 314 322
doi:10.1016/j.envexpbot.2005.03.008

Murashige T Skoog F 1962 A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15 473 497

Paquin R Lechasseur P 1979 Observations sur une methode de dosage de la proline libre dans les extraits de plantes. Canadian Journal of Botany 57 1851 1854


Pelah D Wang W Altman A Shoseyov O Bartles D 1997 Differential accumulation of water stress-related proteins, sucrose synthase and soluble sugars in Populus species that differ in their water stress response. Physiologia Plantarum 99 153 159
doi:10.1111/j.1399-3054.1997.tb03443.x

Ramanjulu S Bartels D 2002 Drought- and desiccation-induced modulation of gene expression in plants. Plant, Cell & Environment 25 141 151 doi:10.1046/j.0016-8025.2001.00764.x

Reid JL Walker-Simmons MK 1993 Group 3 late embryogenesis abundant proteins in desiccation tolerant seedlings of wheat (Triticum aestivum L.). Plant Physiology 102 125 131

Ristic Z Jenks MA 2002 Leaf cuticle and water loss in maize lines differing in dehydration avoidance. Journal of Plant Physiology 159 645 651
doi:10.1078/0176-1617-0743

Sivamani E Bahieldin A Wraith JM Al-Niemi T Dyer WE Ho T-HD Qu RD 2000 Improved biomass productivity and water use efficiency under water deficit conditions in transgenic wheat constitutively expressing the barley HVA1 gene. Plant Science 155 1 9 doi:10.1016/S0168-9452(99)00247-2

Smith JAC , Griffiths H (1993) ‘Water deficits: plant responses from cell to community.’ pp. 1–332. (Bios Scientific Publishers: Oxford, UK)

Suprunova T Krugman T Fahima T Chen G Shams I Korol A Nevo E 2004 Differential expression of dehydrin genes in wild barly, Hordeum spontaneum, associated with resistance to water deficit. Plant, Cell & Environment 27 1297 1308 doi:10.1111/j.1365-3040.2004.01237.x

Turkan I Bor M Ozdemir F Koca H 2005 Differential responses of lipid peroxidation and antioxidants in the leaves of drought-tolerant P. acutifolius Gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Science 168 223 231 doi:10.1016/j.plantsci.2004.07.032

Uma S Ravishankar KV Prasad TG Reid JL Udayakumar M 1993 Abscisic acid responsive proteins induce salinity stress tolerance in fingermillet (Eleusine coracana Gaertn.). Current Science 65 549 554

Vierling E Nguyen HT 1992 Heat-shock protein gene expression in diploid wheat genotypes differing in thermal tolerance. Crop Science 32 370 377


Xu D Duan X Wang B Hong B Ho THD Wu R 1996 Expression of a late embryogenesis abundant protein gene, HVA1, from barley confers tolerance to water deficit and salt stress in transgenic rice. Plant Physiology 110 249 257


Zhu B Choi DW Fenton R Close TJ 2000 Expression of the barley dehydrin multigene family and the development of freezing tolerance. Molecular and General Genetics 264 145 153
doi:10.1007/s004380000299



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