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RESEARCH ARTICLE

A walk on the wild side: mining wild wheat and barley collections for rust resistance genes

Brian J. Steffenson A D , Pablo Olivera A , Joy K. Roy A , Yue Jin B , Kevin P. Smith C and Gary J. Muehlbauer C
+ Author Affliations
- Author Affliations

A Department of Plant Pathology, University of Minnesota, St. Paul, MN 55108, USA.

B United States Department of Agriculture-Agricultural Research Service, Cereal Disease Laboratory, St. Paul, MN 55108, USA.

C Department of Agronomy and Plant Genetics, University of Minnesota, St. Paul, MN 55108, USA.

D Corresponding author. Email: bsteffen@umn.edu

Australian Journal of Agricultural Research 58(6) 532-544 https://doi.org/10.1071/AR07123
Submitted: 26 March 2007  Accepted: 24 May 2007   Published: 26 June 2007

Abstract

Leaf rust, stem rust, and stripe rust are among the most important diseases of wheat and barley worldwide and are best controlled using genetic resistance. To increase the diversity of rust resistance in wheat and barley, a project was initiated to identify and characterise rust resistance genes from the wild species of Aegilops sharonensis (Sharon goatgrass) and Hordeum vulgare ssp. spontaneum (wild barley), respectively. One hundred and two accessions of Sharon goatgrass from Israel and 318 Wild Barley Diversity Collection (WBDC) accessions from the Fertile Crescent, Central Asia, North Africa, and the Caucasus region were evaluated for resistance to leaf rust, stem rust, and/or stripe rust. Sharon goatgrass exhibited a wide range of infection types (ITs) in response to leaf rust, stem rust, and stripe rust. The percentage of resistant accessions in Sharon goatgrass was 58.8–78.4% for leaf rust, 11.8–69.6% for stem rust, and 46.1% for stripe rust, depending on the race used and the plant growth stage. Genetic studies with Sharon goatgrass revealed oligogenic resistance to leaf rust and stem rust. Wild barley also exhibited a wide range of ITs to leaf rust and stem rust; however, the overall frequency of resistance was lower than for Sharon goatgrass. The percentage of resistant accessions in wild barley was 25.8% for leaf rust and 5.7–20.1% for stem rust, depending on the race used. Resistance to the new virulent stem rust race TTKS (i.e. Ug99), present in eastern Africa, was found in both Sharon goatgrass (70% of accessions) and wild barley (25% of 20 accessions tested). Association mapping for stem rust resistance was applied in the WBDC using Diversity Arrays Technology (DArT) markers. Using the highly conservative P value threshold of 0.001, 14 and 15 significant marker associations were detected when the number of subpopulations (K value) was set for 10 and 8, respectively. These significant associations were in 9 and 8 unique chromosome bins, respectively. Two significant marker associations were detected for resistance to the wheat stem rust race MCCF in the same bin as the rpg4/Rpg5 complex on chromosome 7(5H). The presence of a major stem rust resistance gene in this bin on chromosome 7(5H) was validated in a bi-parental mapping population (WBDC accession Damon × cv. Harrington) constructed with DArT markers. The results from this study indicate that Sharon goatgrass and wild barley are rich sources of rust resistance genes for cultivated wheat and barley improvement, respectively, and that association mapping may be useful for positioning disease resistance genes in wild barley.

Additional keywords: allele mining, linkage disequilibrium, wild species, disease resistance.


Acknowledgments

This research was funded by the United States Agency for International Development (USAID) program on Comparative Cereal Genomics and the Lieberman-Okinow Endowment Chair at the University of Minnesota. We thank Stephanie Dahl and Tamas Szinyei for excellent technical assistance, Dr James Kolmer for assistance in the leaf rust evaluations, Dr Jacob Manisterski for assistance in the stripe rust evaluation, and Koffi Adragni for statistical assistance. We are indebted to Dr Yehoshua Anikster for suggesting the Sharon goatgrass project and providing the germplasm used in this study. We thank Dr Andrzej Kilian, Ben Alsop, and Jason Carling for their assistance in genotyping the wild barley collection. BJS dedicates this paper to Professor Robert A. McIntosh for his valuable mentoring.


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