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Plant sciences, sustainable farming systems and food quality
RESEARCH ARTICLE

Breeding triple rust resistant wheat cultivars for Australia using conventional and marker-assisted selection technologies

H. S. Bariana A C , G. N. Brown A , U. K. Bansal A , H. Miah A , G. E. Standen A and M. Lu B
+ Author Affiliations
- Author Affiliations

A USPBI-Cobbitty, Faculty of Agriculture and Natural Resources, PMB11, Camden, NSW 2570, Australia.

B Australian Grain Technologies, USPBI-Narrabri, PO Box 219, Narrabri, NSW 2390, Australia.

C Corresponding author. Email: harbansb@camden.usyd.edu.au

Australian Journal of Agricultural Research 58(6) 576-587 https://doi.org/10.1071/AR07124
Submitted: 26 March 2007  Accepted: 23 May 2007   Published: 26 June 2007

Abstract

Stem rust susceptibility of European wheats under Australian conditions posed a significant threat to wheat production for the early British settlers in Australia. The famous Australian wheat breeder, William Farrer, tackled the problem of stem rust susceptibility through breeding fast-maturing wheat cultivars. South-eastern Australia suffered a severe stem rust epidemic in 1973, which gave rise to a national approach to breeding for rust resistance. The National Wheat Rust Control Program was set up in 1975, modelled on the University of Sydney’s own rust resistance breeding program, at the University of Sydney Plant Breeding Institute, Castle Hill (now Cobbitty). Back-crossing of a range of sources of resistance provided genetically diverse germplasm for evaluation in various breeding programs. Current efforts are directed to building gene combinations through marker-assisted selection. Major genes for resistance to stem rust and leaf rust are being used in the back-crossing program of the ACRCP to create genetic diversity among Australian germplasm. Stripe rust and to a lesser extent leaf rust resistance in the Australian germplasm is largely based on combinations of adult plant resistance genes and our knowledge of their genomic locations has increased. Additional genes, other than Yr18/Lr34 and Yr29/Lr46, appeared to control adult plant resistance to both leaf rust and stripe rust. Two adult-plant stem rust resistance genes have also been identified. The development of selection technologies to achieve genotype-based selection of resistance gene combinations in the absence of bioassays has evolved in the last 5 years. Robust molecular markers are now available for several commercially important rust resistance genes. Marker-assisted selection for rust resistance is performed routinely in many wheat-breeding programs. Modified pedigree and limited back-cross methods have been used for breeding rust-resistant wheat cultivars in the University of Sydney wheat-breeding program. The single back-cross methodology has proved more successful in producing cultivars with combinations of adult plant resistance genes.

Additional keywords: rust diseases, resistance, DNA markers, breeding.


Acknowledgments

We thank GRDC Australia for financial support and Mr T. Watts for providing AWB receivable information. We thank Drs R. P. Singh and R. Wanyera for screening Australian wheat cultivars in Kenya under the Global Rust Initiative and N. Willey, K. Kaur, and M. Gill, for excellent technical assistance. The first author thanks the Value Added Wheat CRC for sponsoring his talk in the ‘Global Landscapes of Cereal Rust Control’, which formed the basis for this publication. Finally, we thank Prof. Bob McIntosh for his continuous encouragement, thought-provoking discussions, and mentorship. This publication would not have been possible without the continuous interaction with all Australian wheat breeders, molecular biologists and pathologists.


References


Akbari M, Wenzl P, Caig V, Carling J, Xia L , et al. (2006) Diversity arrays technology (DArT) for high throughput profiling of hexploid wheat genome. Theoretical and Applied Genetics 113, 1409–1420.
CrossRef | PubMed | open url image1

Bariana HS (2003) Breeding for disease resistance. In ‘Encyclopedia of applied plant sciences’. (Eds B Thomas, DJ Murphy, BG Murray) pp. 244–253. (Academic Press: Harcourt, UK)

Bariana HS , Bell JA , Standen GE , Kaur K (2001 b) Comparative evaluation of wheat cultivars carrying Sr30 against avirulent and virulent Puccinia graminis f. sp. tritici pathotypes. In ‘Proceedings of the 10th Assembly of Wheat Breeding Society of Australia’. Mildura, Vic. (Eds R Eastwood, G Hollamby, A Rathjen, N Gororo) pp. 230–233. (Wheat Breeding Society of Australia)

Bariana HS, Brown GN, Ahmed NU, Khatkar S, Conner RL, Wellings CR, Haley S, Sharp PJ, Laroche A (2002) Characterization of Triticum vavilovii-derived stripe rust resistance using genetic, cytogenetic and molecular analyses and its marker-assisted selection. Theoretical and Applied Genetics 104, 315–320.
CrossRef | PubMed | open url image1

Bariana HS , Cupitt CF , Warburton T (2001 a) Diversity of resistance to rust diseases in Australian wheats in 1999 and 2000 crop seasons. In ‘Proceedings of the 10th Assembly of Wheat Breeding Society of Australia’. Mildura, Vic. (Eds R Eastwood, G Hollamby, A Rathjen, N Gororo) pp. 233–236. (Wheat Breeding Society of Australia)

Bariana HS, McIntosh RA (1995) Genetics of adult plant stripe rust resistance in four Australian wheat cultivars and French cultivar Hybride-de-Bersee. Plant Breeding 114, 485–491.
CrossRef | open url image1

Bariana HS , Park RF , Wellings CR (2003) ‘Cereal cultivars, rust reactions and other information,’ Australian Cereal Rust Control Program Circular #44, pp.34. (University of Sydney Plant Breeding Institute: Cobbitty, NSW)

Bariana HS , Willey NJ , Venkata BP , Lehmenseik A , Standen GE , Lu M (2004) Breeding methodology to achieve durability for rust resistance in wheat. In ‘Proceedings of the 54th Australian Cereal Chemistry Conference and 11th Wheat Breeders Assembly’. Canberra, ACT. (Eds CK Black, JF Panozzo, GJ Rebetzke) pp. 8–12. (Wheat Breeding Society of Australia)

Bossolini E, Krattinger SG, Keller B (2006) Development of simple sequence repeat markers specific for the Lr34 resistance region of wheat using sequence information from rice and Aegilops tauschii. Theoretical and Applied Genetics 113, 1049–1062.
CrossRef | PubMed | open url image1

Brennan JP, Murray GM (1988) Australian wheat diseases—assessing their economic importance. Agricultural Science New Series 1, 26–35. open url image1

Brown GN (1997) The inheritance and expression of leaf chlorosis associated with gene Sr2 for adult plant resistance to wheat stem rust. Euphytica 95, 67–71.
CrossRef | open url image1

Cristobal U, Brevis JC, Chen X, Khan I, Jackson L, Chicaiza O, Distefeld A, Fahima T, Dubcovsky J (2005) High temperature adult plant (HTAP) stripe rust resistance gene Yr36 from Triticum turgidum ssp. dicoiccoides is closely linked to the grain protein content locus Gpc-B1. Theoretical and Applied Genetics 112, 97–105.
CrossRef | PubMed | open url image1

Dubin HJ, Rajaram S (1996) Breeding disease resistant wheats for tropical highlands and lowlands. Annual Review of Phytopathology 34, 503–526.
CrossRef | PubMed | open url image1

Dundas IS , Bariana HS , Park RF , Islam AKMR , McIntosh RA , Shepherd KW (2001) An update on recent progress and an outline of an expanded program. In ‘Proceedings of the 10th Assembly of Wheat Breeding Society of Australia’. MIldura, Vic. (Eds R Eastwood, G Hollamby, A Rathjen, N Gororo) pp. 44–47. (Wheat Breeding Society of Australia)

Dundas IS , Shepherd KW , McIntosh RA (1999) Progress towards improving the performance of wheat lines carrying alien stem rust resistance genes Sr26, Sr32 and Sr37 using molecular cytogenetics. In ‘Proceedings of the 9th Assembly of Wheat Breeding Society of Australia’. Toowoomba, Qld. (Ed. PM Williamson) pp. 141–143. (Wheat Breeding Society of Australia)

Dundas IS , Verlin DC , Park RF , Bariana HS , Anugrahwati DR , Shepherd KW , McIntosh RA , Islam AKMR (2004) Progress in development of new rust resistant wheat using chromosomes from uncultivated relatives. In ‘Proceedings of the 54th Australian Cereal Chemistry Conference and 11th Wheat Breeders Assembly’. Canberra, ACT. (Eds CK Black, JF Panozzo, GJ Rebetzke) pp. 122–124. (Wheat Breeding Society of Australia)

Ellingboe AH (1981) Changing concept of host-pathogen genetics. Annual Review of Phytopathology 19, 125–143.
CrossRef | open url image1

Gold J, Harder D, Townley-Smith F, Aung T, Procunier J (1999) Development of a molecular marker for rust resistance genes Sr39 and Lr35 in wheat breeding. Nature Biotechnology 2, open url image1

Hare RA, McIntosh RA (1979) Genetic and cytogenetic studies of durable adult-plant resistances in Hope and related cultivars to wheat rusts. Zeitschrift Pflanzenzuchtung 83, 350–367. open url image1

Hayden MJ, Kuchel H, Chalmers KJ (2004) Sequence tagged microsetellites for the Xgwm533 locus provide new diagnostic markers to select for the presence of stem rust resistance gene Sr2 in bread wheat (Triticum aestivum L.). Theoretical and Applied Genetics 109, 1641–1647.
CrossRef | PubMed | open url image1

Helguera M, Khan IA, Dubcovsky J (2000) Development of PCR markers for wheat leaf rust resistance gene Lr47. Theoretical and Applied Genetics 101, 625–631.
CrossRef | open url image1

Helguera M, Khan IA, Kolmer J, Lijavetzky D, Zhong-qi L, Dubcovsky J (2003) PCR assays for the Lr37-Yr17-Sr38 cluster of rust resistance genes and their use to develop isogenic hard red spring wheat lines. Crop Science 43, 1839–1847. open url image1

Huang L, Gill BS (2001) An RGA like marker detects all known Lr21 leaf rust resistance gene family members in Aegilops taushii and wheat. Theoretical and Applied Genetics 103, 1007–1013.
CrossRef | open url image1

Johnson R (1983) Genetic background of durable resistance. In ‘Durable resistance in crops’. (Eds F Lamberti, JM Walker, NA van der Graff) pp. 5–26. (Plenum Press: New York and London)

Johnson R, Law CN (1973) Cytogenetic studies on the resistance of the bread wheat variety Bersee to Puccinia striiformis. Cereal Rusts Bulletin 1, 38–43. open url image1

Johnson R, Taylor AJ (1972) Isolates of Puccinia striiformis collected in England from wheat varieties Maris Beacon and Joss Cambier. Nature 238, 105–106.
CrossRef | open url image1

Kaur J , Bariana HS (2004) Inheritance of adult plant resistance to stripe rust in Australian wheat cultivars Kukri and Sunco. In ‘Proceedings of the 54th Australian Cereal Chemistry Conference and 11th Wheat Breeders Assembly’. Canberra, ACT. (Eds CK Black, JF Panozzo, GJ Rebetzke) pp. 19–21. (Cereal Chemistry Division, Royal Australian Chemical Institute: Melbourne)

Khan RR, Bariana HS, Dholakia BB, Naik SY, Lagu MD, Rathjen AJ, Bhavani S, Gupta V (2005) Molecular mapping of stem rust and leaf rust resistance in wheat. Theoretical and Applied Genetics 111, 846–850.
CrossRef | PubMed | open url image1

Knott DR (1989) ‘The wheat rusts – breeding for resistance.’ Monograph on Theoretical and Applied Genetics 12. (Springer Verlag: Belin)

Kuraparthy V, Chhuneja P, Dhaliwal HS, Kaur S, Bowden RL, Gill BS (2007) Characterization and mapping of cryptic alien introgression from Aegilops geniculata with new leaf rust and stripe rust resistance genes Lr57 and Yr40 in wheat. Theoretical and Applied Genetics 114, 1379–1389.
CrossRef | PubMed | open url image1

Lagudah ES, Mcfadden H, Singh RP, Heurta-Espino J, Bariana HS, Spielmyer W (2006) Molecular genetic characterization of the Lr34/Yr18 slow rusting resistance gene region in wheat. Theoretical and Applied Genetics 114, 21–30.
CrossRef | PubMed | open url image1

Luig NH (1979) Mutation studies in Puccinia graminis tritici. In ‘Proceedings of the 5th International Wheat Genetics Symposium’. New Delhi. (Ed. S Ramanujam) pp. 533–539. (Indian Society of Genetics and Plant Breeding: New Delhi, India)

Mago R, Bariana HS, Dundas LS, Speilmeyer W, Lawrence GJ, Pryor AJ, Ellis JG (2005) Development of PCR markers for the selection of wheat stem rust resistance genes Sr24 and Sr26 in diverse wheat germplasm. Theoretical and Applied Genetics 111, 496–504.
CrossRef | PubMed | open url image1

Mago R, Speilmeyer W, Lawrence GJ, Lagudah ES, Ellis JG, Pryor AJ (2002) Identification and mapping of molecular markers linked to rust resistance genes located on chromosome 1RS of rye using wheat rye translocation lines. Theoretical and Applied Genetics 104, 1317–1324.
CrossRef | PubMed |
open url image1

Mago R, Speilmeyer W, Lawrence GJ, Ellis JG, Pryor AJ (2004) Resistance genes for rye stem rust (SrR) and barley powdery mildew (Mla) are located in syntenic regions on short arm of chromosome 1. Genome 47, 112–121.
CrossRef | PubMed | open url image1

Marais GF, McCallum B, Snyman JE, Pretorius ZA, Marais AS (2005b) Leaf rust and stripe rust resistance genes Lr54 and Yr37 transferred to wheat from Aegilops kotschyi. Plant Breeding 124, 538–541.
CrossRef | open url image1

Marais GF, McCallum B, Marais AS (2006) Leaf rust and stripe rust resistance genes derived from Aegilops Sharonensis. Euphytica 149, 373–380.
CrossRef | open url image1

Marais GF, Pretorius ZA, Wellings CR, McCallum B, Marais AS (2005a) Leaf rust and stripe rust resistance genes transferred to common wheat from Triticum dicoccoides. Euphytica 143, 115–123.
CrossRef | open url image1

McIntosh RA (1991) Pre-emptive breeding to control wheat rusts. In ‘Proceedings of the International Conference on Breeding for Disease Resistance’. Newcastle-Upon-Tyne, UK. (Eds R Johnson, GJ Jellis) pp. 103–114. (Kluwer Academic Publishers: Dordrecht, The Netherlands)

McIntosh RA, Brown GN (1997) Anticipatory breeding for resistance to rust diseases in wheat. Annual Review of Phytopathology 35, 311–326.
CrossRef | PubMed |
open url image1

McIntosh RA , Luig NH (1973) Recombination between genes for reaction to P. graminis at or near the Sr9 locus. In ‘Proceedings of the 4th International Wheat Genetics Symposium’. (Eds ER Sears, LM Sears) pp. 425–432. (Agricultural Experiment Station, University of Missouri: Columbia, MO)

McIntosh RA , Yamazaki Y , Devos KM , Dubcovsky J , Rogers WJ , Appels R (2003) Catalogue of gene symbols for wheat. In ‘Proceedings of the 10th International Wheat Genetics Symposium’. Paestum, Italy. (Eds NE Pogna, M Romano, A Pogna, G Galterio)

Metzger RJ, Silbaugh BA (1970) Inheritance of resistance to stripe rust and its association with glume colour in Triticum aestivum L. P.I. 178383. Crop Science 10, 567–568. open url image1

Miah H (2004) Genetics of rust resistance in wheat and barley. PhD thesis, The University of Sydney, Australia.

Miller TE, Reader SM, Ambrose MJ (2000) The Watkins wheat collection. Annual Wheat Newsletter 46, 172. open url image1

O’Brien L, Nrown JS, Pascoe I (1980) Occurrence and distribution of wheat stripe rust in Victoria and susceptibility of commercial wheat cultivars. Australasian Plant Pathology 9, 14.
CrossRef | open url image1

Park RF, Bariana HS, Wallwork H, Wellings CR (2002) Detection of virulence for leaf rust resistance gene Lr24 in Australia. Australian Journal of Agricultural Research 53, 1069–1076.
CrossRef | open url image1

Pathan AK (2003) Genetics of resistance to rust diseases in European and New Zealand wheat cultivars. PhD thesis, The University of Sydney, Australia.

Pathan AK, Park RF (2006) Evaluation of seedling and adult plant resistance to leaf rust in European wheat cultivars. Euphytica 149, 327–342.
CrossRef | open url image1

Penrose LDJ, Walsh K, Clark K (1998) Characters contributing to high yield in Currawong, an Australian winter wheat. Australian Journal of Agricultural Research 49, 853–866.
CrossRef | open url image1

Pretorius ZA, Singh RP, Wagoire WW, Payne TS (2000) Detection of virulence to wheat stem rust resistance gene Sr31 in Puccinia graminis f. sp. tritici in Uganda. Plant Disease 84, 203.
CrossRef | open url image1

Prins R, Groenewald JZ, Marais GF, Snape JW, Koebner RMD (2001) AFLP and STS tagging of Lr19, a gene conferring resistance to leaf rust in wheat. Theoretical and Applied Genetics 103, 618–624.
CrossRef | open url image1

Singh D, Park RF, McIntosh RA (2001) Inheritance of seedling and adult plant resistance to leaf rust of selected Australian spring and English winter wheat varieties. Plant Breeding 120, 503–507.
CrossRef | open url image1

Singh RP (1992) Association between gene Lr34 for leaf rust resistance and leaf tip necrosis in wheat. Crop Science 32, 874–878. open url image1

Singh RP , Huerta-Espino J (2004) The use of ‘single backcross, selected bulk’ breeding approach for transferring minor genes based rust resistance into adapted cultivars. In ‘Proceedings of the 54th Australian Cereal Chemistry Conference and 11th Wheat Breeders Assembly’. Canberra, ACT. (Eds CK Black, JF Panozzo, GJ Rebetzke) pp. 48–51. (Cereal Chemistry Division, Royal Australian Chemical Institute: Melbourne)

Singh RP, Huerta-Espino J, Rajaram S (2000) Achieving near-immunity to leaf and stripe rusts in wheat by combining slow rusting resistance genes. Acta Phytopathologica et Entomologica Hungarica 35, 133–139. open url image1

Spielmeyer W, Sharp PJ, Lagudah ES (2003) Identification and validation of markers linked to broad spectrum stem rust resistance gene Sr2 in wheat (Triticum aestivum L.). Crop Science 43, 333–336. open url image1

The TT , Latter BDH , McIntosh RA , Ellison FW , Brennan PS , Fisher J , Hollamby GJ , Rathjen AJ , Wilson RE (1988) Grain yields of near-isogenic lines with added genes for stem rust resistance. In ‘Proceedings of the 7th International Wheat Genetics Symposium’. Cambridge, England. (Eds TE Miller, RMD Koebner) pp. 901–906. (Institute of Plant Science: Cambridge, UK)

Wang L, Ma J, Ronghua , Wang X, Jia J (2002) Molecular tagging of the yellow rust resistance gene Yr10 in common wheat P.I.178383 (Triticum aestivum L.). Euphytica 124, 71–73.
CrossRef | open url image1

Watson IA, Frankel OH (1972) Walter Lawry Waterhouse. Records of the Australian Academy of Science 2, 16. open url image1

Watson IA, Singh D (1952) The future for rust resistant wheat in Australia. Journal of Australian Institute of Agricultural Science 18, 190–197. open url image1

Wellings CR (2007) Puccinia striiformis in Australia: a review of the incursion, evolution, and adaptation of stripe rust in the period 1979–2005. Australian Journal of Agricultural Research 58, 567–575.
CrossRef |
open url image1

Wellings CR, Wright DG, Keiper F, Loughman R (2003) First detection of wheat stripe rust in Western Australia: evidence for a foreign incursion. Australasian Plant Pathology 32, 321–322.
CrossRef | open url image1

Zwer P, Park RF, McIntosh RA (1992) Wheat stem rust in Australia – 1969–1965. Australian Journal of Agricultural Research 43, 399–431.
CrossRef | open url image1








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