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RESEARCH ARTICLE
Contents Vol 61(1)

Characterisation of high- and low-molecular-weight glutenin subunit genes in Chinese winter wheat cultivars and advanced lines using allele-specific markers and SDS-PAGE

F. P. Yang A B G , L. H. Wang A G , J. W. Wang A C , X. Y. He A , X. K. Zhang C , X. W. Shang D , W. X. Yang B , X. C. Xia A F and Z. H. He A E F
+ Author Affiliations
- Author Affiliations

A Institute of Crop Science, National Wheat Improvement Centre/The National Key Facility for Crop Gene Resources and Genetic Improvement, Chinese Academy of Agricultural Sciences (CAAS), 12 Zhongguancun South Street, Beijing 100081, China.

B Crop Research Institute, Gansu Academy of Agricultural Sciences, Lanzhou 730070, China.

C College of Agronomy, Northwest Sci-Tech University of Agriculture and Forestry, Yangling, Shaanxi 712100, China.

D College of Agronomy, Gansu Agricultural University, Lanzhou 730070, China.

E International Maize and Wheat Improvement Centre (CIMMYT) China Office, c/o CAAS, 12 Zhongguancun South Street, Beijing 100081, China.

F Corresponding authors. Emails: xiaxianchun@caas.net.cn; zhhe@public3.bta.net.cn

G F. P. Yang and L. H. Wang contributed equally to this work.

Crop and Pasture Science 61(1) 84-91 https://doi.org/10.1071/CP09164
Submitted: 9 June 2009  Accepted: 16 September 2009   Published: 17 December 2009

Abstract

Wheat end-use product quality is highly influenced by the composition and quantity of high- and low-molecular-weight glutenin subunits (HMW-GS and LMW-GS). In the present study, 224 Chinese wheat cultivars and advanced lines were characterised for the HMW-GS and LMW-GS with allele-specific PCR markers and sodium-dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE). The results showed that 56 cultivars (25.0%) carried the allele Glu-D1-1d (Dx5), while 80 cultivars (35.7%) with the allele Glu-B1-2a (By8) produced a 527-bp specific band. Fourteen genotypes (6.3%) with the allele Glu-B1e (Bx20) yielded a 701-bp amplicon with the marker Mar and a 753-bp specific PCR fragment with the marker ZSBy9aF1/R3. Glu-B1h (Bx14+By15) was present in only 1 genotype, and 2 cultivars contained the allele Glu-B1f (Bx13+By16) identified with the marker ZSBy9F2/R2. Four genotypes (1.8%) with the allele Glu-B1-1d (Bx6) gave 695-bp and 830-bp bands, and 5 genotypes (2.2%) with the allele Glu-B1i (Bx17+By18) amplified a 659-bp fragment using the marker Bx. One hundred and six cultivars (47.3%) had the allele Glu-B1-2b (By9), amplifying a 663-bp fragment with the marker ZSBy9aF1/R3; 34 genotypes (15.8%) contained the allele Glu-B3d, generating a 662-bp PCR fragment with the marker gluB3d. Fifteen cultivars (7.0%) with the allele Glu-B3b yielded 1570-bp and 750-bp PCR amplicons with the markers gluB3b and gluB3bef, respectively. The allele Glu-B3h was found in 7 cultivars, generating a 1022-bp PCR fragment with the marker gluB3h. The genotypes detected by SDS-PAGE were mostly consistent with those identified by molecular markers, confirming the utility of the molecular markers. The information for the HMW-GS and LMW-GS in Chinese wheat cultivars will be useful in wheat breeding programs.

Additional keywords: Triticum aestivum L., HMW-GS, LMW-GS, SDS-PAGE.


Acknowledgments

The authors are very grateful to Prof. Bob McIntosh, at the Plant Breeding Institute, University of Sydney, for his critical review of this manuscript. This study was supported by the National 863 Programs (2006AA10Z1A7 and 2006AA100102), and the International Collaboration Project from the Ministry of Agriculture (2006-G2).


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