Crop and Pasture Science Crop and Pasture Science Society
Plant sciences, sustainable farming systems and food quality
RESEARCH ARTICLE

Chromosome composition of an F2 Triticum aestivum × T. turgidum spp. durum cross analysed by DArT markers and MCFISH

Friederike S. Eberhard A , Peng Zhang B , Anke Lehmensiek A , Ray A. Hare C , Steven Simpfendorfer C and Mark W. Sutherland A D

A Centre for Systems Biology, University of Southern Queensland, Toowoomba, Qld 4350, Australia.

B University of Sydney, Plant Breeding Institute, Cobbitty, NSW 2570, Australia.

C New South Wales Industry and Investment, Tamworth Agricultural Institute, Tamworth, NSW 2340, Australia.

D Corresponding author. Email: mark.sutherland@usq.edu.au

Crop and Pasture Science 61(8) 619-624 http://dx.doi.org/10.1071/CP10131
Submitted: 16 April 2010  Accepted: 20 June 2010   Published: 13 August 2010

Abstract

This study has employed multicolour fluorescence in situ hybridisation (MCFISH) and Diversity Arrays Technology (DArT) markers to determine the segregation of parental A, B and D genome material into the progeny of a cross between a hexaploid bread wheat (Triticum aestivum L. var. 2-49) and a tetraploid durum wheat [T. turgidum L. spp. durum (Desf.) var. Bellaroi]. In the F2 progeny from a 2-49/Bellaroi cross, 82 out of 83 F2 plants investigated with DArT analysis carried some D genome material, principally as entire chromosomes, while 40 plants included at least one complete copy of all seven D genome chromosomes. Twelve plants containing partial D chromosomes were identified. MCFISH analysis of 26 additional F2 plants of the same cross showed that all 26 plants contained varying amounts of D genome material of which three carried single A-D translocations. In addition two telocentric D genome chromosomes were detected. The D genome content of each line and the breakpoint positions of the three A-D translocations were confirmed with DArT marker analysis. Overall results indicate a random recombination of A and B genome loci from the hexaploid female parent and the tetraploid male parent in this F2 population and a significant retention of the maternal D genome material. This study illustrates that the combined application of the MCFISH and DArT techniques provides a powerful approach for the analysis of crosses between cereal genotypes of different ploidy.

Additional keywords: DArT, durum wheat, hexaploid × durum crosses, MCFISH.


References

Akbari M Wenzl P Caig V Carling J Xia L Yang S Uszynski G Mohler V Lehmensiek A Kuchel H Hayden MJ Howes N Sharp P Vaughan P Rathmell B Huttner E Kilian A 2006 Diversity arrays technology (DArT) for high-throughput profiling of the hexaploid wheat genome. Theoretical and Applied Genetics 113 1409 1420 doi:10.1007/s00122-006-0365-4

Aoki T O’Donnell K 1999 Morphological and molecular characterization of Fusarium pseudograminearum sp. nov., formerly recognized as the Group 1 population of F. graminearum. Mycologia 91 597 609 doi:10.2307/3761245

Boeuf C Prodanovic G Gay G Bernard M 2003 Structural organization of the group-1 chromosomes of two bread wheat sister lines. Theoretical and Applied Genetics 106 938 946 doi:10.1007/s00122-002-1154-3

Ceoloni C Biagetti M Ciaffi M Forte P Pasquini M 1996 Wheat chromosome engineering at the 4× level: the potential of different alien gene transfers into durum wheat. Euphytica 89 87 97 doi:10.1007/BF00015724

Collard BCY Grams RA Bovill WD Percy CD Jolley R Lehmensiek A Wildermuth GB Sutherland MW 2005 Development of molecular markers for crown rot resistance in wheat: mapping of QTLs for seedling resistance in a ‘2-49’ × ‘Janz’ population. Plant Breeding 124 532 537 doi:10.1111/j.1439-0523.2005.01163.x

Gilbert J Procunier JD Aung T 2000 Influence of the D genome in conferring resistance to fusarium head blight in spring wheat. Euphytica 114 181 186 doi:10.1023/A:1004065620127

Kihara H 1925 Weitere Untersuchungen über die pentaploiden Triticum-Bastarde. I. Japanese Journal of Botany 2 299 305

Kihara H (1982) ‘Wheat studies, retrospect and prospects.’ (Kodansha Ltd: Tokyo)

Kumar S Gill BS Faris JD 2007 Identification and characterization of segregation distortion loci along chromosome 5B in tetraploid wheat. Molecular Genetics and Genomics 278 187 196
doi:10.1007/s00438-007-0248-7

Lanning SP Blake NK Sherman JD Talbert LE 2008 Variable production of tetraploid and hexaploid progeny lines from spring wheat by durum wheat crosses. Crop Science 48 199 202 doi:10.2135/cropsci2007.06.0334

Manly KF Cudmore RHJ Meer JM 2001 Map Manager QTX, cross-platform software for genetic mapping. Mammalian Genome 12 930 932 doi:10.1007/s00335-001-1016-3

Pogna N , Mazza R (1996) ‘Gluten quality and storage protein composition of durum wheat lines containing the Gli-D1/Glu-D3 loci.’ (C. W. Wrigley, Cereal Chemistry Division, Royal Australian Chemical Institute: North Melbourne, Vic.)

Sax K 1922 Sterility in wheat hybrids II: Chromosome behaviour in partially sterile hybrids. Genetics 7 513 585

Thompson WP Hollinghead I 1927 Preponderance of dicoccum-like characters and chromosome numbers in hybrids between T. dicoccum and T. vulgare. Genetics 17 283 307
doi:10.1007/BF02983132

Van Os H Stam P Visser RGF Van Eck HJ 2005 RECORD: a novel method for ordering loci on a genetic linkage map. Theoretical and Applied Genetics 112 30 40 doi:10.1007/s00122-005-0097-x

Wang HY Liu DC Yan ZH Wei YM Zheng YL 2005 Cytological characteristics of F2 hybrids between Triticum aestivum L. and T. durum Desf. with reference to wheat breeding. Journal of Applied Genetics 46 365 369

Wenzl P , Carling J , Kudrna D , Jaccoud D , Huttner E , Kleinhofs A , Kilian A (2004) Diversity arrays technology (DArT) for whole-genome profiling of barley. Proceedings of the National Academy of Sciences of the United States of America 101, 9915–9920.
doi:10.1073/pnas.0401076101

Zhang P Friebe B Lukaszewski AJ Gill BS 2001 The centromere structure in Robertsonian wheat-rye translocation chromosomes indicates that centric breakage-fusion can occur at different positions within the primary constriction. Chromosoma 110 335 344 doi:10.1007/s004120100159

Zhang P Li W Friebe B Gill BS 2004 Simultaneous painting of three genomes in hexaploid wheat by BAC-FISH. Genome 47 979 987 doi:10.1139/g04-042



Export Citation