Invertebrate Systematics Invertebrate Systematics Society
Systematics, phylogeny and biogeography
RESEARCH ARTICLE

Molecular barcode and morphological analyses reveal the taxonomic and biogeographical status of the striped-legged hermit crab species Clibanarius sclopetarius (Herbst, 1796) and Clibanarius vittatus (Bosc, 1802) (Decapoda : Diogenidae)

Mariana Negri A , Leonardo G. Pileggi A and Fernando L. Mantelatto A B

A Laboratory of Bioecology and Crustacean Systematics, Postgraduate Program in Comparative Biology, Department of Biology, Faculty of Philosophy, Science and Letters of Ribeirão Preto (FFCLRP), University of São Paulo (USP), Brazil. Email: ma_negri90@hotmail.com, lpileggi@gmail.com

B Corresponding author. Email: flmantel@usp.br

Invertebrate Systematics 26(6) 561-571 http://dx.doi.org/10.1071/IS12020
Submitted: 4 April 2012  Accepted: 13 September 2012   Published: 19 December 2012

Abstract

The taxonomic status of the species Clibanarius sclopetarius (Herbst, 1796) and Clibanarius vittatus (Bosc, 1802), which have sympatric biogeographical distributions restricted to the western Atlantic Ocean, is based only on differences in the colour pattern of the walking legs of adults. Their morphological similarity led to the suggestion that they be synonymised. In order to investigate this hypothesis, we included species of Clibanarius Dana, 1892 in a molecular phylogenetic analysis of partial sequences of the mitochondrial 16S rDNA gene and the COI barcode region. In addition, we combined the molecular results with morphological observations obtained from several samples of these two species. The genetic divergences of the 16S rDNA and COI sequences between C. sclopetarius and C. vittatus ranged from 4.5 to 5.9% and 9.4 to 11.9%, which did not justify their synonymisation. Differences in the telson morphology, chela ornamentation, and coloration of the eyestalks and antennal peduncle provided support for the separation of the two species. Another interesting result was a considerable genetic difference found between populations of C. vittatus from Brazil and the Gulf of Mexico, which may indicate the existence of two homonymous species.

Additional keywords: 16S rDNA, Anomura, barcoding COI, molecular systematics, western Atlantic.


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