A molecular phylogeny of the subtribe Pterostylidinae (Orchidaceae): resolving the taxonomic confusion
Jasmine K. Janes A C , Dorothy A. Steane A , René E. Vaillancourt A and Marco F. Duretto BA School of Plant Science, University of Tasmania, Private Bag 55, Hobart, Tas. 7001, Australia.
B Tasmanian Herbarium, Tasmanian Museum and Art Gallery, Private Bag 4, Hobart, Tas. 7001, Australia.
C Corresponding author. Email: jkjanes@utas.edu.au
Australian Systematic Botany 23(4) 248-259 https://doi.org/10.1071/SB10006
Submitted: 17 February 2010 Accepted: 3 May 2010 Published: 31 August 2010
Abstract
In the past decade, two major classification schemes of the subtribe Pterostylidinae have resulted in taxonomic confusion because a single well known genus was split into a large number of new genera and subgenera, many of which are difficult to discriminate accurately. These classifications have not been accepted widely among systematists because of poor phylogenetic support for several genera. Analyses of the internal transcribed spacer (ITS) region of nuclear rDNA in a large number of species and samples facilitate further clarification of the relationships within the Pterostylidinae. The phylogenetic trees were reconstructed using parsimony and Bayesian methods. These phylogenetic trees indicate that subtribe Pterostylidinae is monophyletic, and support the concept of a single genus, Pterostylis R.Br. sensu lato within the Pterostylidinae. Two clades representing subgenera correlate with the morphology of the lateral sepals, whereas several of the previously erected genera consistently have poor support. The proposed subgenera are divided further into 10 sections. Several closely related taxa with identical ITS sequences require further scrutiny by population-level molecular techniques to determine their taxonomic status.
Additional keywords: greenhoods, orchids, parsimony, Pterostylis, taxonomy.
Acknowledgements
The authors thank Hans Wapstra for identification confirmation. The authors are indebted to Dr Wendy Potts from the Threatened Species Section (Department Primary Industries and Water, Tas.); and Dr Gay McKinnon and James Marthick from the School of Plant Science (University of Tasmania) for their assistance and support. This research was funded by a Discovery grant (DPO557260) from the Australian Research Council, an Australian Postgraduate Award to the first author and research funding from the Australian Systematic Botany Society (Hansjörg Eichler Scientific Research Fund).
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