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RESEARCH ARTICLE
Contents Vol 59(3)

Molecular barcoding of north-east Atlantic deep-water sharks: species identification and application to fisheries management and conservation

Teresa Moura A C E , Mónica C. Silva B , Ivone Figueiredo A , Ana Neves C , Pablo Durán Muñoz D , Maria Manuela Coelho B and Leonel S. Gordo C
+ Author Affiliations
- Author Affiliations

A Unidade de Recursos Marinhos e Sustentabilidade, Instituto de Investigação das Pescas e do Mar (IPIMAR-INRB), Av. Brasília, 1449-006 Lisboa, Portugal.

B Centro de Biologia Ambiental, Faculdade de Ciências, Universidade de Lisboa, Bloco C2-3°Piso, Campo Grande, 1749-016 Lisboa, Portugal.

C Departamento de Biologia Animal and Instituto de Oceanografia, Faculdade de Ciências, Universidade de Lisboa, Bloco C8, Campo Grande, 1749-016 Lisboa, Portugal.

D Instituto Español de Oceanografía, Programa de Pesquerías Lejanas, Centro Oceanográfico de Vigo, Apartado 1552, Spain.

E Corresponding author. Email: tmoura@ipimar.pt

Marine and Freshwater Research 59(3) 214-223 https://doi.org/10.1071/MF07192
Submitted: 19 October 2007  Accepted: 26 January 2008   Published: 30 April 2008

Abstract

Two genera of elasmobranchii, Centrophorus and Centroscymnus, include species that represent the highest landings of deep-water sharks caught by fishing fleets operating in the north-east Atlantic. There are morphology-based identification problems among and within genera, and landings of processed shark products further prevent an objective assessment of these species. The present study is the first attempt to test the suitability of using a DNA barcode approach to discriminate accurately among the four most important commercial deep-water shark species: Centrophorus squamosus, Centrophorus granulosus, Centroscymnus coelolepis and, recently discovered in Portuguese ports, Centroscymnus owstoni. Sequence analyses of the mitochondrial DNA cytochrome c oxidase subunit I (COI) gene revealed low levels of haplotypic and genetic diversities. Higher levels of inter-specific relative to intra-specific divergences allowed discrimination among species, which form reciprocally monophyletic clades. Inclusion of published COI sequences from other species within the same genera revealed haplotype sharing among species, which calls into question the current taxonomy and accuracy of fisheries data available. Amplification of the COI gene coupled with MboI restriction digests was found to be a fast and inexpensive strategy to resolve within genera identification problems. Molecular barcoding constitutes a critical tool for the assessment and implementation of urgent management policies for this group of species.

Additional keywords: Centrophorus, Centroscymnus, coxI, cytochrome c oxidase subunit I, restriction digest fragment analysis.


Acknowledgements

We would like to thank the members of the M. Coelho laboratory, B. Pereira (IPIMAR) and P. Machado (IPIMAR) for their expertise and help at various stages of this work. We also thank the crew of the ‘AQUIVARI’ (Sesimbra) for the support and providing tissue samples. J. C. Silva, M. A. Aboim, A. R. Amaral provided helpful comments on the manuscript. We are also grateful to Dr Robert Ward, the editor and an anonymous referee for their useful comments and suggestions. T. Moura and M. C. Silva were each funded by grants from the Fundação para a Ciência e a Tecnologia, respectively SFRH/BD/29052/2006 and SFRH/BPD/22276/2005. The present study was partially supported by the Project TUBAPROF (MARE 22–05–01-FEDER-00028, co-financed by the EU) and PNAB EU/DCR. A special thanks to the Project ECOVUL/ARPA 2005/10 of the C. O. de Vigo from the Instituto Español de Oceanografía, which allowed the collection of the Hatton Bank samples.


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