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Advances in the aquatic sciences
RESEARCH ARTICLE

Determining phycoerythrin and phycocyanin concentrations in aqueous crude extracts of red algae

S Beer and A Eshel

Australian Journal of Marine and Freshwater Research 36(6) 785 - 792
Published: 1985

Abstract

The following equations are proposed for correct calculations of pigment concentrations (E, phycoerythrin; C, phycocyanin, mg ml-1 in red algal crude extracts, from absorption measurements:

E=[(A564 - A592) - (A455-A592) 0.20] 0.12,

C=[A618 - A645) - (A592-A645) 0.15] 0.15

These equations are shown to yield results that are not affected by the concentration of interfering components. Fluorometry is also shown to be suitable for phycoerythrin measurements (with much higher sensitivity) in the same type of extracts.

https://doi.org/10.1071/MF9850785

© CSIRO 1985

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