Reproduction, Fertility and Development Reproduction, Fertility and Development Society
Vertebrate reproductive science and technology
RESEARCH ARTICLE

Using cell banks as a tool in conservation programmes of native domestic breeds: the production of the first cloned Anatolian Grey cattle

Sezen Arat A F , Arzu T. Caputcu A , Tolga Akkoc A , Serhat Pabuccuoglu B , Hakan Sagirkaya C , Umut Cirit D , Yavuz Nak C , Evren Koban A , Haydar Bagis A E , Kamber Demir B , Deniz Nak C , Adem Senunver B , Ragip Kilicaslan B , Bilginer Tuna C , Gaye Cetinkaya A , Melis Denizci A and Ozgur Aslan A
+ Author Affiliations
- Author Affiliations

A The Scientific and Technological Research Council of Turkey, Marmara Research Center, Genetic Engineering and Biotechnology Institute, 41470 Gebze, Kocaeli, Turkey.

B Istanbul University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination and Department of Obstetrics and Gynecology, Avcilar, Istanbul, Turkey.

C Uludag University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination and Department of Obstetrics and Gynecology, Gorukle, Bursa, Turkey.

D Dicle University, Faculty of Veterinary Medicine, Department of Reproduction and Artificial Insemination, Diyarbakir, Turkey.

E Adiyaman University, Faculty of Medicine, Department of Medical Genetics, Adiyaman, Turkey.

F Corresponding author. Emails: sezen.arat@mam.gov.tr; sezenarat@yahoo.com

Reproduction, Fertility and Development 23(8) 1012-1023 https://doi.org/10.1071/RD11026
Submitted: 24 January 2011  Accepted: 6 May 2011   Published: 22 September 2011

Abstract

The aim of this study was to clone native Anatolian Grey cattle by using different donor cell types, such as fibroblast, cartilage and granulosa cells cryopreserved in a gene bank and oocytes aspirated from ovaries of Holstein cows as the recipient cytoplasm source. One male calf from fibroblast, three female calves from granulosa cells and one female calf from cartilage cells were born healthy and at normal birthweights. No calves were lost after birth. The results demonstrated that the cloned calves had the same microsatellite alleles at 11 loci as their nuclear donors. However, the mtDNAs of the five Anatolian Grey cloned calves had different haplotypes from their donor cells and mtDNA heteroplasmy could not be detected in any of the clones. The birth of healthy clones suggests that the haplotype difference between the cell and oocyte donor did not affect the pre- or post-implantation development of the bovine nuclear transfer derived embryos in our study. The results showed that well established nuclear transfer protocols could be useful in conserving endangered species. In conclusion, somatic cell banking can be suggested as a tool in conservation programmes of animal genetic resources.

Additional keywords: cloning, cryopreservation, haplotypes, mtDNA, nuclear transfer, telomere.


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