Forelimb bone growth and mineral maturation as potential indices of skeletal maturity in sheep
M. A. Cake A C , G. E. Gardner A B , M. D. Boyce A , D. Loader A and D. W. Pethick AA School of Veterinary and Biomedical Sciences, Murdoch University, Murdoch, WA 6150, Australia.
B School of Rural Science and Natural Resources, University of New England, Armidale, NSW 2351, Australia.
C Corresponding author. Email: mcake@murdoch.edu.au
Australian Journal of Agricultural Research 57(6) 699-706 https://doi.org/10.1071/AR05111
Submitted: 22 March 2005 Accepted: 18 June 2005 Published: 20 June 2006
Abstract
The aim of this study was to characterise the allometric growth and bone mineral maturation of forelimb bones in sheep throughout the growth phase. Forelimb bones (scapula to proximal phalanx) were measured in 84 Merino sheep from similar genetic stock of approximately 12, 32, 64, 84, 116, and 168 weeks of age, with approximately equal numbers of wethers and ewes in each age cohort (n = 14). Sheep were selected for divergence of size, body weight, and condition, in order that the effects of age and body size could be assessed independently. Bone magnesium was measured in the metacarpal and humerus. Results demonstrate the highly coordinated, allometric nature of linear bone growth within the ovine forelimb, though allometric growth patterns differed from those previously published for bone weights. We propose that estimates of maturity proportion (M) based on relative limb bone length or limb proportions may present significant advantages over weight- or composition-based maturity indices, or qualitative variables such as dental eruption or USDA-type maturity scores. Sex differences in growth gradients were minimal, although the higher variability and greater gender divergence of metacarpal bone length casts doubt on the use of its growth plate (breakjoint) to indicate maturity. Bone magnesium content was found to decrease rapidly during the growth period and may represent a useful independent estimate of physiological maturity.
Additional keywords: allometric growth, bone magnesium.
Acknowledgments
The authors thank Dr Kevin Bell and Bill Webb for assistance in sourcing the sheep, and Dr Robin Jacob for his assistance with slaughters. The constructive assistance of Dr John Thompson with modelling procedures is acknowledged. This project was funded by the Australian Sheep Industry CRC.
Alfrey AC,
Miller NL, Trow R
(1974) Effect of age and magnesium depletion on bone magnesium pools in rats. Journal of Clinical Investigation 54, 1074–1081.
| PubMed |
Beighle D,
Boyazoglu P,
Hemken R, Serumaga-Zake P
(1994) Determination of calcium, phosphorus, and magnesium values in rib bones from clinically normal cattle. American Journal of Veterinary Research 55, 85–89.
| PubMed |
Berg R, Butterfield R
(1966) Muscle : bone ratio and fat percentage as measures of beef carcass composition. Animal Production 8, 1–11.
Butterfield R,
Griffiths D,
Thompson J,
Zamora J, James A
(1983) Changes in body composition relative to weight and maturity in large and small strains of Australian Merino rams. 1. Muscle, bone and fat. Animal Production 36, 29–37.
Davies AS
(1975) A comparison of tissue development in Pietrain and large white pigs from birth to 64 kg live weight. Animal Production 20, 45–49.
Davies AS, Kallweit E
(1979) The effect of body weight and maturity on the carcass composition of the pig. Zeitschrift fur Tierzuchtung und Zuchtungsbiologie 96, 6–7.
Davies AS,
Tan GY, Broad TE
(1984) Growth gradients in the skeleton of cattle, sheep, and pigs. Zentralblatt für Veterinärmedizin. Reihe C. Anatomia, Histologia, Embryologia 13, 222–230.
Di Masso RJ,
Celoria GC, Font MT
(1998) Morphometric skeletal traits, femoral measurements, and bone mineral deposition in mice with agonistic selection for body conformation. Bone 22, 539–543.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Fretz P,
Cymbaluk N, Pharr J
(1984) Quantitative analysis of long-bone growth in the horse. American Journal of Veterinary Research 45, 1603–1609.
Grynpas M
(1993) Age and disease-related changes in the mineral of bone. Calcified Tissue International Suppl 1 53, S57–S64.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Ho L,
Field R,
Russell W,
Riley M,
Ercanbrack S, Williams F
(1989) Influence of gender, breed, and age on maturity characteristics of sheep. Journal of Animal Science 67, 2460–2470.
| PubMed |
Hooper A
(1977) Effects of divergent selection for body weight on bone length and diameter in mice. Animal Production 24, 77–82.
Jeremiah L,
Tong A, Gibson L
(1997) The influence of lamb chronological age, slaughter weight, and gender on carcass and meat quality. Sheep and Goat Research Journal 13, 96–104.
Kirton AH, O’Hara P
(1975) Determination of age of lamb carcasses from pelvic ossification. Animal Production 21, 257–264.
Kline S,
Hotchkiss R,
Randolph M, Weiland A
(1990) Study of growth kinetics and morphology in limbs transplanted between animals of different ages. Plastic and Reconstructive Surgery 85, 273–280.
| PubMed |
Lawrence T,
Whatley J,
Montgomery T, Perino L
(2001) A comparison of the USDA ossification-based maturity system to a system based on dentition. Journal of Animal Science 79, 1683–1690.
| PubMed |
McClelland T,
Boniati B, Taylor SC
(1976) Breed differences in body composition of equally mature sheep. Animal Production 23, 281–293.
Oberbauer AM,
Arnold AM, Thonney ML
(1994) Genetically size-scaled growth and composition of Dorset and Suffolk rams. Animal Production 59, 223–234.
Oberbauer AM,
Currie WB,
Krook L, Thonney ML
(1989) Endocrine and histologic correlates of the dynamics of the metacarpal growth plate in growing rams. Journal of Animal Science 67, 3124–3135.
| PubMed |
Oberbauer AM,
Krook L,
Hogue DE,
Currie WB, Thonney ML
(1988) Dietary calcium and metacarpal growth in ewes. Journal of Nutrition 118, 976–981.
| PubMed |
Oishi A,
Hamada S,
Sakamoto H,
Kamiya S,
Yanagida K,
Kubota C,
Watanabe Y, Shimizu R
(1996) Radiographical evaluation of bone maturation in Japanese Black beef cattle. Journal of Veterinary Medical Science 58, 529–535.
| PubMed |
Peralta JM,
Arnold AM,
Currie WB, Thonney ML
(1994) Effects of testosterone on skeletal growth in lambs as assessed by labeling index of chondrocytes in the metacarpal bone growth plate. Journal of Animal Science 72, 2629–2634.
| PubMed |
Perry D,
Thompson J, Butterfield R
(1992) Bone distribution patterns in sheep selected for high and low weaning weight. Animal Production 54, 129–135.
Ravaglioli A,
Krajewski A,
Celotti G,
Piancastelli A,
Bacchini B,
Montanari L,
Zama G, Piombi L
(1996) Mineral evolution of bone. Biomaterials 17, 617–622.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Richmond RJ,
Jones SDM,
Price MA, Berg RT
(1979) Effects of breed and sex on the relative growth and distribution of bone in pigs. Canadian Journal of Animal Science 59, 471–479.
Smith R
(1956) Fusion of the epiphyses of the limb bones of the sheep. The Veterinary Record 68, 257–259.
Stevens D,
Boyer M, Bowen C
(1999) Transplantation of epiphyseal plate allografts between animals of different ages. Journal of Pediatric Orthopedics 19, 398–403.
| Crossref | GoogleScholarGoogle Scholar | PubMed |
Taylor St CS
(1980) Genetically standardized growth equations. Animal Production 30, 167–175.
USDA
(1982) Standards for the grades of lamb, yearling mutton and mutton carcasses. Federal Regulations 47, 40141.
Wenham G, Pennie K
(1986) The growth of individual muscles and bones in the red deer. Animal Production 42, 247–256.