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RESEARCH ARTICLE

Increasing sucrose accumulation in sugarcane by manipulating leaf extension and photosynthesis with irrigation

N. G. Inman-Bamber A , G. D. Bonnett B , M. F. Spillman A , M. L. Hewitt C and J. Jackson A
+ Author Affiliations
- Author Affiliations

A CSIRO Sustainable Ecosystems, Davies Laboratory, University Road, Townsville, Qld., Australia.

B CSIRO Plant Industry, Queensland Bioscience Precinct, 306 Carmody Road, St Lucia, Qld., Australia.

C CSIRO Plant Industry, Davies Laboratory, University Road, Townsville, Qld., Australia.

Australian Journal of Agricultural Research 59(1) 13-26 https://doi.org/10.1071/AR07167
Submitted: 24 April 2007  Accepted: 27 August 2007   Published: 14 January 2008

Abstract

High sucrose content (SC) in sugarcane stalks is a priority for all sugarcane industries world wide. Partitioning to sucrose in the cane stalk is related to the supply of photo-assimilate and the demand for assimilate by other organs. If photosynthesis could be maintained, but leaf and stalk growth constrained, by genetics or management during the stalk elongation phase, it may be possible to reduce stalk height and to increase both SC and sucrose yield. This paper reports an experiment designed to test this hypothesis and to develop a methodology to assess variation in response to source–sink manipulation in sugarcane clones. The research was conducted on a ‘low’ (Q138) and a ‘high’ (Q183) SC cultivar in two temperature controlled and airtight glasshouses (chambers) at CSIRO’s Davies Laboratory in Townsville, Australia. Potted plants of each cultivar were placed in two chambers of the Tall Plant Facility (TPF). In one chamber, plants were irrigated to minimise water stress while plants in the other chamber were irrigated to reduce plant extension rate (PER) considerably more than photosynthesis.

Water stress reduced gain in total biomass by 19% and gain in top mass by 37%, and increased sucrose mass gain by 27%. During the experiment, SC of dry matter increased 37% in the dry treatment and only 8% in the wet treatment and this effect was greater in Q183 than in Q138. Water stress reduced whole plant photosynthesis by 18%, thus largely accounting for the 19% reduction in biomass accumulation and it reduced PER by 41%, corresponding to the 37% reduction in mass of tops. Reduced PER resulted in reduced demand for photo-assimilate by fibre and tops thus allowing excess assimilate to accumulate in the form of sucrose. The techniques developed here to control PER and measure the resulting changes in carbon partitioning now allow further examination of both the control of the balance between growth and sucrose storage and the extent of genotypic variation to the response of reduced PER.

Additional keywords: source–sink, dry matter partitioning, water stress.


Acknowledgments

This research was funded by the Australian Federal Government and Sugar Industry through the Sugar Research and Development Cooperation. The authors are grateful to Professor Bob Lawn and Dr Sarah Park for their informed comments on the manuscript.


References


Aitken KS, Jackson PA, McIntyre CL (2006) QTL identified for sugar related traits in a sugarcane (Saccharum spp.) cultivar × S. officinarum population. Theoretical and Applied Genetics 112, 1306–1317.
Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Albertson PL, Grof CPL (2007) Application of high performance anion exchange-pulsed amperometric detection to measure the activity of key sucrose metabolising enzymes in sugarcane. Journal of Chromatography 845, 151–156.
Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Berding N (1997) Clonal improvement of sugarcane based on selection for moisture content: fact or fiction. Proceedings of the Australian Society of Sugar Cane Technologists 19, 245–253. open url image1

Bonnett GD, Hewitt ML, Glassop D (2006) Effects of temperature on the growth and composition of sugarcane internodes. Australian Journal of Agricultural Research 57, 1087–1095.
Crossref | GoogleScholarGoogle Scholar | open url image1

Botha FC, Black KG (2000) Sucrose phosphate synthase and sucrose synthase activity during maturation of internodal tissue in sugarcane. Australian Journal of Plant Physiology 27, 81–85. open url image1

Casu RE, Dimmock CM, Chapman SC, Grof CPL, McIntyre CL, Bonnett GD, Manners JM (2004) Identification of differentially expressed transcripts from maturing stem of sugarcane by in silico analysis of stem expressed sequence tags and gene expression profiling. Plant Molecular Biology 54, 503–517.
Crossref | GoogleScholarGoogle Scholar | PubMed | open url image1

Casu RE, Manners JM, Bonnett GD, Jackson PA, McIntyre CL, Dunne R, Chapman SC, Rae AL, Grof CPL (2005) Genomics approaches for the identification of genes determining important traits in sugarcane. Field Crops Research 92, 137–148.
Crossref | GoogleScholarGoogle Scholar | open url image1

Clements HF (1980) ‘Sugarcane crop logging and crop control: principles and practices.’ (Pitman Publishing Ltd: London)

Glasziou KT, Gayler KR (1972) Storage of sugars in stalks of sugarcane. Botanical Review 38, 471–488. open url image1

Holden JR (1998) Irrigation of sugarcane. Queensland, Bureau of Sugar Experiment Stations, Indooroopilly, Qld, Australia.

Inman-Bamber NG (1995) Measured and simulated plant stress criteria for the irrigation of sugarcane. Proceedings of the South African Irrigation Symposium 1991, 144–148. open url image1

Inman-Bamber NG (2004) Sugarcane water stress criteria for irrigation and drying off. Field Crops Research 89, 107–122.
Crossref | GoogleScholarGoogle Scholar | open url image1

Inman-Bamber NG, Smith MD (2005) Water relations in sugarcane and response to water deficits. Field Crops Research 92, 185–202.
Crossref | GoogleScholarGoogle Scholar | open url image1

Inman-Bamber NG, Spillman MF (2002) Plant extension, soil water extraction and water stress in sugarcane. Proceedings of the Australian Society of Sugar Cane Technologists 24, 242–256. open url image1

Inman-Bamber NG, Muchow RC, Robertson MJ (2002) Dry matter partitioning of sugarcane in Australia and South Africa. Field Crops Research 76, 71–84.
Crossref | GoogleScholarGoogle Scholar | open url image1

Jackson PA (2005) Progress and prospects in genetic improvement in sucrose accumulation. Field Crops Research 92, 277–290.
Crossref | GoogleScholarGoogle Scholar | open url image1

Jackson PA, Bonnett G, Chudleigh P, Hogarth M, Wood A (2000) The relative importance of cane yield and traits affecting CCS in sugarcane varieties. Proceedings of the Australian Society of Sugar Cane Technologists 22, 23–29. open url image1

Keating BA, Robertson MJ, Muchow RC, Huth NI (1999) Modelling sugarcane production systems 1. Development and performance of the sugarcane module. Field Crops Research 61, 253–271.
Crossref | GoogleScholarGoogle Scholar | open url image1

Moore PH (2005) Integration of sucrose accumulation processes across hierarchical scales: towards developing an understanding of the gene-to-crop continuum. Field Crops Research 92, 119–135.
Crossref | GoogleScholarGoogle Scholar | open url image1

Rae AL, Bonnett GD, Karno (2006) Understanding stem development and sucrose accumulation to increase CCS. Proceedings of the Australian Society of Sugar Cane Technologists 28, 327–335. open url image1

Rae AL, Grof CPL, Casu RE, Bonnett GD (2005) Sucrose accumulation in the sugarcane stem: pathways and control points for transport and compartmentation. Field Crops Research 92, 159–168.
Crossref | GoogleScholarGoogle Scholar | open url image1

Rawson HM, Hindmarsh JH, Fischer RA, Stockman YM (1983) Changes in leaf photosynthesis with plant ontogeny and relationships with yield per ear in wheat cultivars and 120 progeny. Australian Journal of Plant Physiology 10, 503–514. open url image1

Robertson MJ, Bonnett GD, Hughes RM, Muchow RC, Campbell JA (1998) Temperature and leaf area expansion of sugarcane: integration of controlled-environment, field and model studies. Australian Journal of Plant Physiology 25, 819–828. open url image1

Robertson MJ, Donaldson RA (1998) Changes in the components of cane and sucrose yield in response to drying off before harvest. Field Crops Research 55, 201–208.
Crossref | GoogleScholarGoogle Scholar | open url image1

Singels A, Bezuidenhout CN (2002) A new method of simulating dry matter partitioning in the Canegro sugarcane model. Field Crops Research 78, 151–164.
Crossref | GoogleScholarGoogle Scholar | open url image1

Singels A, Smit MA, Redshaw KA, Donaldson RA (2005) The effect of crop start date, crop class and cultivar on sugarcane canopy development and radiation interception. Field Crops Research 92, 249–260.
Crossref | GoogleScholarGoogle Scholar | open url image1

Singh G, Chapman SC, Jackson PA, Lawn RJ (2002) Lodging reduces sucrose accumulation of sugarcane in the wet and dry tropics. Australian Journal of Agricultural Research 53, 1183–1195.
Crossref | GoogleScholarGoogle Scholar | open url image1

Van Dillewijn C (1952) ‘Botany of sugarcane.’ (Chronica Botanica: Waltham, MA)

Vu JCV, Allen LH, Gesch RW (2006) Up-regulation of photosynthesis and sucrose metabolism enzymes in young expanding leaves of sugarcane under elevated growth CO2. Plant Science 171, 123–131.
Crossref | GoogleScholarGoogle Scholar | open url image1

Watt DA, McCormick AJ, Govender C, Carson DL, Cramer MD, Huckett BI, Botha FC (2005) Increasing the utility of genomics in unravelling sucrose accumulation. Field Crops Research 92, 149–158.
Crossref | GoogleScholarGoogle Scholar | open url image1